An approach to evaluating a generalized index of the radiation effect of NFC objects on biota is proposed. The effect of the radiation factor on the collection of representative natural organisms belonging to difference taxonomic groups is taken into account. The change in the proposed index for individual actinides fl owing with activity 1 MBq into the environment and their decay products is calculated. The actinides present in spent fuel are considered in the calculations: 241,242m,242,243 Am,[242][243][244][245][246][247][248] 237 Np,[238][239][240][241][242]244 Pu, and 235,236,238 U. The index developed is suitable for radioecological assessment of the objects of open and closed fuel cycles. The results of such assessment are an important element of the environmental validation of new nuclear power technologies.A condition for the development of nuclear power, together with economic competitiveness, full use of the raw material potential, and technological support of the nonproliferation regime is environmental acceptability [1]. The objects of the nuclear fuel cycle (NFC) have a multifactorial effect on the environment. However, society focuses on the radiation factor, which is specifi c to nuclear power generation [2].The most important environmental problem is to reduce the activity of long-lived high-level wastes which are subject to disposal. Their activity can be reduced by means of transmutation technologies [3]. The objective is to attain equality of the potential biological danger of the wastes and natural raw material (comparison of the initial and fi nal stages of the fuel cycle) [4]. In developing this concept taking account of the dose loads on the general population and the biota one can talk about striving toward radioecological equivalence in the raw-material-wastes system [5].In a more general formulation of the problem, on the level of management of radioactive materials in a complete cycle it is expedient to use the principle of integral comparative assessment of different scenarios of the development of nuclear power [6]. This principle consists in the evaluation of the radioecological safety of open and closed NFC taking account of the following: 1) the set of objects comprising the NFC (from mining the raw materials to disposal of the wastes); 2) different regimes of functioning of NFC objects, including analysis of the consequences of accidental emissions; and 3) effect on not only humans but also living objects of the environment. In this formulation, the problem can be defi ned as radioecological validation of new nuclear energy technologies, which is of complex and research character, in contrast to the problems associated with radiation monitoring or prediction of the consequences of radioactive emissions for particular objects of the NFC [7,8].