Radiation chemists look at damage in redox proteins induced by X‐rays

Wherland, Scot; Pecht, Israel

doi:10.1002/prot.25521

Cited by 6 publications

(5 citation statements)

References 85 publications

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“…In metal containing proteins the photoelectrons can reduce the metal center and change the rst surrounding shell [67]. These changes have been investigated by pulsed radiolysis and by XANES measurements on Mn containing centers [68].…”

Section: Nature and Life Time Of Free Radicals And Electronic Defects...mentioning

confidence: 99%

When x-rays alter the course of your experiments*

Bras

Myles

Felici³

2021

J. Phys.: Condens. Matter

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The continuing increase in the brilliance of synchrotron radiation beamlines allows for many new and exciting experiments that were impossible before the present generation of synchrotron radiation sources came on line. However, the exposure to such intense beams also tests the limits of what samples can endure. Whilst the effects of radiation induced damage in a static experiment often can easily be recognized by changes in the diffraction or spectroscopy curves, the influence of radiation on chemical or physical processes, where one expects curves to change, is less often recognized and can be misinterpreted as a ‘real’ result instead of as a ‘radiation influenced result’. This is especially a concern in time-resolved materials science experiments using techniques as powder diffraction, small angle scattering and x-ray absorption spectroscopy. Here, the effects of radiation (5–50 keV) on some time-resolved processes in different types of materials and in different physical states are discussed. We show that such effects are not limited to soft matter and biology but rather can be found across the whole spectrum of materials research, over a large range of radiation doses and is not limited to very high brilliance beamlines.

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Section: Nature and Life Time Of Free Radicals And Electronic Defects...mentioning

confidence: 99%

When x-rays alter the course of your experiments*

Bras

Myles

Felici³

2021

J. Phys.: Condens. Matter

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“…The issue becomes especially relevant when dealing with non-invasive monitoring of small and/or delicate samples, 7 accurate analysis of surfaces, 8 and investigation of biological diluted samples. 9 Examples of the last type are proteins in complex with transition metal ions 10 as their oxidation state can change under radiation, 11 also because of chemical reactions with naturally abundant reducing agents. 12,13 Even in cases where expressly designed sample holders for XAS measurements under controlled electrochemical conditions are employed, 14 or experimental approaches combining low temperature, sample motion and limited doses are employed, 15 good monitoring of damage is required for an accurate analysis.…”

Section: Introductionmentioning

confidence: 99%

Dealing with Cu reduction in X-ray absorption spectroscopy experiments

et al. 2019

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“…The issue becomes especially relevant when dealing with noninvasive monitoring of small and/or delicate samples, [92] accurate analysis of surfaces, [93] and investigation of biological diluted samples. [94] Examples of the latter are proteins in complexes with transition metal ions, [95] as their oxidation state can change under radiation, [96] and because of chemical reactions with naturally abundant reducing agents. [97,98]…”

Section: Sources Of Errormentioning

confidence: 99%

Experimental methods in chemical engineering:X‐ray absorption spectroscopy—XAS,XANES,EXAFS

et al. 2021

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Although X-ray absorption spectroscopy (XAS) was conceived in the early 20th century, it took 60 years after the advent of synchrotrons for researchers to exploit its tremendous potential. Counterintuitively, researchers are now developing bench type polychromatic X-ray sources that are less brilliant to measure catalyst stability and work with toxic substances. XAS measures the absorption spectra of electrons that X-rays eject from the tightly bound core electrons to the continuum. The spectrum from 10 to 150 eV (kinetic energy of the photoelectrons) above the chemical potential-binding energy of core electrons-identifies oxidation state and band occupancy (X-ray absorption near edge structure, XANES), while higher energies in the spectrum relate to local atomic structure like coordination number and distance, Debye-Waller factor, and inner potential correction (extended X-ray absorption fine structure, EXAFS). Combining XAS with complementary spectroscopic techniques like Raman, Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS), and electron paramagnetic resonance (EPR) elucidates the nature of the chemical bonds at the catalyst surface to better understand reaction mechanisms and intermediates. Because synchrotrons continue to be the light source of choice for most researchers, the number of articles Web of Science indexes per year has grown from 1000 in 1991 to 1700 in 2020. Material scientists and physical chemists publish an order of magnitude articles more than chemical engineers. Based on a bibliometric analysis, the research comprises five clusters centred around: electronic and optical properties, oxidation and hydrogenation catalysis, complementary analytical techniques like FTIR, nanoparticles and electrocatalysis, and iron, metals, and complexes.

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Radiation chemists look at damage in redox proteins induced by X‐rays

Cited by 6 publications

References 85 publications

When x-rays alter the course of your experiments*

When x-rays alter the course of your experiments*

Dealing with Cu reduction in X-ray absorption spectroscopy experiments

Experimental methods in chemical engineering:X‐ray absorption spectroscopy—XAS,XANES,EXAFS

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