Radiation damage studies in cardiac muscle cells and tissue using microfocused X-ray beams: experiment and simulation

Nicolas, Jan-David; Aeffner, Sebastian; Salditt, Tim

doi:10.1107/s1600577519006817

Cited by 14 publications

(36 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, freeze-dried CMs can be imaged with relatively high sampling in real space, since less free radicals are produced in the absence of water/buffer. More importantly, free radicals do not spread by diffusion as in hydrated environments (21). In Fig.…”

Section: Characterization Of the Actomyosin Latticementioning

confidence: 90%

“…This is the case for a pristine spot. Since previous exposures of neighboring scan points can induce a 'bystander effect', the step size is a further important parameter and has to be chosen sufficiently large (21). Therefore, this scheme results in rather coarse-grained real space maps of the cells.…”

Section: Data Acquisition and Image Analysis Scanning Saxs Measurementsmentioning

confidence: 99%

“…Cellular samples are commonly described by H 50 C 30 N 9 O 10 S. For the used experimental parameters this yields a mass density = 1.35 g cm 3 and a mass attenuation length = 7.46 • 10 −4 m (51, 52). The choice of image acquisition parameters for diffraction experiments was guided by our previous investigation and simulation of radiation damage (21). The average dose per shot in the diffraction experiments was = 5.3 • 10 5 Gy.…”

Section: Dose Calculationmentioning

confidence: 99%

“…This enables recordings of the small-angle x-ray scattering (SAXS) while scanning the sample in real space, as initially introduced for biomaterials (11,12), more recently also for soft matter (13), as well as for 3d vector SAXS (14). Scanning diffraction from biological cells with spot sizes smaller than a single organelle are still more challenging in terms of signal-to-noise (15)(16)(17)(18)(19)(20), as well as radiation damage (21). Recently, we have used this approach to study the cytoskeletal structure of single CMs in order to compare the signal level for different preparation states (22).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

X-ray structural analysis of single adult cardiomyocytes: tomographic imaging and micro-diffraction

Reichardt¹,

Neuhaus²,

Nicolas³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

ABSTRACTWe present a multi-scale imaging approach to characterize the structure of isolated adult murine cardiomyocytes based on a combination of full-field three-dimensional (3d) coherent x-ray imaging and scanning x-ray diffraction. Using these modalities, we probe the structure from the molecular to the cellular scale. Holographic projection images on freeze-dried cells have been recorded using highly coherent and divergent x-ray waveguide radiation. Phase retrieval and tomographic reconstruction then yield the 3d electron density distribution with a voxel size below 50 nm. In the reconstruction volume, myofibrils, sarcomeric organisation and mitochondria can be visualized and quantified within a single cell without sectioning. Next, we use micro-focusing optics by compound refractive lenses to probe the diffraction signal of the acto-myosin lattice. Comparison between recordings of chemically fixed and untreated, living cells indicate that the characteristic lattice distances shrink by approximately 10% upon fixation.SIGNIFICANCEDiffraction with synchrotron radiation has played an important role to decipher the molecular structure underlying force generation in muscle. In this work, the diffraction signal of the actomyosin contractile unit has for the first time been recorded from living cardiomyocytes, bringing muscle diffraction to the scale of single cells. In addition to scanning diffraction, we use coherent optics at the same synchrotron endstation to perform holographic imaging and tomography on a single cardiomyocyte. By this hard x-ray microscopy modality, we extend the length scales covered by scanning diffraction and reconstruct the electron density of an entire freeze-dried cardiomyocyte, visualizing the 3d architecture of myofibrils, sarcomers, and mitochondria with a voxel size below 50 nm.

show abstract

Section: Characterization Of the Actomyosin Latticementioning

confidence: 90%

Section: Data Acquisition and Image Analysis Scanning Saxs Measurementsmentioning

confidence: 99%

Section: Dose Calculationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

X-ray structural analysis of single adult cardiomyocytes: tomographic imaging and micro-diffraction

Reichardt¹,

Neuhaus²,

Nicolas³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…SAXS was also used, but in scanning mode (sSAXS), to analyse the parameters affecting radiation damage rates in RT cardiac muscle cells and tissue (Nicolas et al, 2019), by monitoring the intensity of the reflection from the acto-mysin lattice following microbeam (< 3 mm) irradiation. The vari-ables investigated included the scan step size, the length of the horizontal scan, the vertical distance between scan lines and the exposure time per scan point.…”

Section: # 2019 International Union Of Crystallographymentioning

confidence: 99%

X-ray radiation damage to biological samples: recent progress

Garman

Weik

2019

J Synchrotron Rad

View full text Add to dashboard Cite

With the continuing development of beamlines for macromolecular crystallography (MX) over the last few years providing ever higher X‐ray flux densities, it has become even more important to be aware of the effects of radiation damage on the resulting structures. Nine papers in this issue cover a range of aspects related to the physics and chemistry of the manifestations of this damage, as observed in both MX and small‐angle X‐ray scattering (SAXS) on crystals, solutions and tissue samples. The reports include measurements of the heating caused by X‐ray irradiation in ruby microcrystals, low‐dose experiments examining damage rates as a function of incident X‐ray energy up to 30 keV on a metallo‐enzyme using a CdTe detector of high quantum efficiency as well as a theoretical analysis of the gains predicted in diffraction efficiency using these detectors, a SAXS examination of low‐dose radiation exposure effects on the dissociation of a protein complex related to human health, theoretical calculations describing radiation chemistry pathways which aim to explain the specific structural damage widely observed in proteins, investigation of radiation‐induced damage effects in a DNA crystal, a case study on a metallo‐enzyme where structural movements thought to be mechanism related might actually be radiation‐damage‐induced changes, and finally a review describing what X‐ray radiation‐induced cysteine modifications can teach us about protein dynamics and catalysis. These papers, along with some other relevant literature published since the last Journal of Synchrotron Radiation Radiation Damage special issue in 2017, are briefly summarized below.

show abstract

Chemo-mechanical-microstructural coupling in the tarsus exoskeleton of the scorpion Scorpio palmatus

et al. 2023

View full text Add to dashboard Cite

Radiation damage studies in cardiac muscle cells and tissue using microfocused X-ray beams: experiment and simulation

Cited by 14 publications

References 54 publications

X-ray structural analysis of single adult cardiomyocytes: tomographic imaging and micro-diffraction

X-ray structural analysis of single adult cardiomyocytes: tomographic imaging and micro-diffraction

X-ray radiation damage to biological samples: recent progress

Chemo-mechanical-microstructural coupling in the tarsus exoskeleton of the scorpion Scorpio palmatus

Contact Info

Product

Resources

About