Radiation Degradation of β-Glucan with a Potential for Reduction of Lipids and Glucose in the Blood of Mice

Long, Nguyễn Thành; Ánh, Nguyễn Thị Ngọc; Bach, Long Giang; Sơn, Hoàng Nghĩa; Luân, Lê Quang

doi:10.3390/polym11060955

Cited by 21 publications

(5 citation statements)

References 48 publications

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“…Previous studies have established the relationship between reduction and maintenance of blood glucose levels, and pA1c [13], PC [26,27] and BGC [28], indicating the presumed potential activity of the probiotic and the two bioactive compounds in the regulation of glucose metabolism and insulin sensitivity. Therefore, we evaluated the synergic effect of our synbiotic combinations on mammalian glucose homeostasis and insulin sensitivity in C57BL/6J mice with diabetes and diet-induced obesity.…”

Section: The Synbiotics Combinations Including Pa1c Reduce Fbg and Si...mentioning

confidence: 95%

Glucose-lowering effects of a synbiotic combination containing Pediococcus acidilactici in C. elegans and mice

Yavorov-Dayliev,

Milagro,

Ayo

et al. 2023

Diabetologia

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Aims/hypothesis Modulation of gut microbiota has emerged as a promising strategy to treat or prevent the development of different metabolic diseases, including type 2 diabetes and obesity. Previous data from our group suggest that the strain Pediococcus acidilactici CECT9879 (pA1c) could be an effective probiotic for regulating glucose metabolism. Hence, the objectives of this study were to verify the effectiveness of pA1c on glycaemic regulation in diet-induced obese mice and to evaluate whether the combination of pA1c with other normoglycaemic ingredients, such as chromium picolinate (PC) and oat β-glucans (BGC), could increase the efficacy of this probiotic on the regulation of glucose and lipid metabolism. Methods Caenorhabditis elegans was used as a screening model to describe the potential synbiotic activities, together with the underlying mechanisms of action. In addition, 4-week-old male C57BL/6J mice were fed with a high-fat/high-sucrose diet (HFS) for 6 weeks to induce hyperglycaemia and obesity. Mice were then divided into eight groups (n=12 mice/group) according to dietary supplementation: control-diet group; HFS group; pA1c group (1010 colony-forming units/day); PC; BGC; pA1c+PC+BGC; pA1c+PC; and pA1c+BGC. Supplementations were maintained for 10 weeks. Fasting blood glucose was determined and an IPGTT was performed prior to euthanasia. Fat depots, liver and other organs were weighed, and serum biochemical variables were analysed. Gene expression analyses were conducted by real-time quantitative PCR. Sequencing of the V3–V4 region of the 16S rRNA gene from faecal samples of each group was performed, and differential abundance for family, genera and species was analysed by ALDEx2R package. Results Supplementation with the synbiotic (pA1c+PC+BGC) counteracted the effect of the high glucose by modulating the insulin–IGF-1 signalling pathway in C. elegans, through the reversal of the glucose nuclear localisation of daf-16. In diet-induced obese mice, all groups supplemented with the probiotic significantly ameliorated glucose tolerance after an IPGTT, demonstrating the glycaemia-regulating effect of pA1c. Further, mice supplemented with pA1c+PC+BGC exhibited lower fasting blood glucose, a reduced proportion of visceral adiposity and a higher proportion of muscle tissue, together with an improvement in the brown adipose tissue in comparison with the HFS group. Besides, the effect of the HFS diet on steatosis and liver damage was normalised by the synbiotic. Gene expression analyses demonstrated that the synbiotic activity was mediated not only by modulation of the insulin–IGF-1 signalling pathway, through the overexpression of GLUT-1 and GLUT-4 mediators, but also by a decreased expression of proinflammatory cytokines such as monocyte chemotactic protein-1. 16S metagenomics demonstrated that the synbiotic combinations allowed an increase in the concentration of P. acidilactici, together with improvements in the intestinal microbiota such as a reduction in Prevotella and an increase in Akkermansia muciniphila. Conclusions/interpretation Our data suggest that the combination of pA1c with PC and BGC could be a potential synbiotic for blood glucose regulation and may help to fight insulin resistance, diabetes and obesity. Graphical Abstract

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Section: The Synbiotics Combinations Including Pa1c Reduce Fbg and Si...mentioning

confidence: 95%

Glucose-lowering effects of a synbiotic combination containing Pediococcus acidilactici in C. elegans and mice

Yavorov-Dayliev,

Milagro,

Ayo

et al. 2023

Diabetologia

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“…It is notable that the irradiated degraded β-polysaccharide, whose molecular weight became 11 to 48 kDa, had a stronger effect in reducing blood lipids and blood glucose in mice, with a significant decrease in all indicators. Among them, irradiated β-polysaccharide with a molecular weight of about 25 kDa showed the strongest reduction in all tested indicators (47.2% in total cholesterol, 45.7% in LDL cholesterol and 45.5% in triglycerides) ( Long et al, 2019 ). Therefore, the use of Fermentum β-polysaccharide with a lower molecular weight should be considered among relevant treatments.…”

Section: Activitymentioning

confidence: 99%

An important polysaccharide from fermentum

Lin

Huang²

2022

Food Chemistry: X

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“…After 24 h of APAP administration, 0.5 ml of blood from the myocardium of the tested mice was collected and placed into tubes containing heparin for analysis. The collected blood was then centrifuged at 4500 × g for 10 min to separate the serum [33]. The obtained serum was used to analyse aspartate aminotransferase (AST) and alanine aminotransferase (ALT) indexes on a Beckman Coulter Au480 analyser (USA).…”

Section: (A)mentioning

confidence: 99%

Synthesis of Carboxymethyl Chitosan-Capped Gold Nanoparticles by Gamma Irradiation with Novel Potential Applications as Antioxidant, Hepatoprotective, and Anticancer Substance

Linh

Nghĩa²,

Vu³

et al. 2021

Journal of Nanomaterials

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Gold nanoparticles capped by carboxymethyl chitosan (AuNPs/CM-chitosan) with particle sizes of 5.2–7.3 nm were successfully synthesized by the γ-irradiation of Au3+ solutions. Their characteristics were analysed by transmission electron microscope images, powder X-ray diffraction patterns, UV-visible spectroscopy, and Fourier transform infrared spectra. The antioxidant activity of AuNP/CM-chitosan was time dependent and much higher than that of ascorbic acid at the same concentration. On the other hand, the results of tail vein injection of AuNP/CM-chitosan in mice indicated that this product was not toxic to mice and that AuNPs were mainly distributed in liver tissue, at approximately 77.5%, 6 h after injection. The hepatoprotective activity of AuNP/CM-chitosan was also tested in acetaminophen-induced hepatotoxic mice by oral administration at daily doses of 0.5–2 mg/head. The results indicated that compared to the control, supplementation with 2 mg of AuNPs/head strongly reduced the aspartate aminotransferase and alanine aminotransferase indexes in the blood of the tested mice by approximately 66.5 and 69.3%, respectively. Furthermore, the MTT (3[4,5 dimetylthiazol-2-yl]-2,5-diphenyltetrazol bromide) assay on a liver cancer cell line (HepG2) clearly confirmed strong anticancer activity on HepG2 cells treated with 0.05–0.5 mM AuNPs and the tested cells did not survive after treatment with 0.5 mM AuNPs, while the growth of the normal cell line (L929) has no significant effect at the same treated concentration of AuNPs. The AuNP/CM-chitosan in the present study was synthesized by the γ-irradiation method without using any toxic-reducing chemical and stabilized in a natural biocompatible polymer. The strong antioxidant, hepatoprotective, and anticancer effects of this product may be supported to be used in the biomedical field.

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Radiation Degradation of β-Glucan with a Potential for Reduction of Lipids and Glucose in the Blood of Mice

Cited by 21 publications

References 48 publications

Glucose-lowering effects of a synbiotic combination containing Pediococcus acidilactici in C. elegans and mice

Glucose-lowering effects of a synbiotic combination containing Pediococcus acidilactici in C. elegans and mice

An important polysaccharide from fermentum

Synthesis of Carboxymethyl Chitosan-Capped Gold Nanoparticles by Gamma Irradiation with Novel Potential Applications as Antioxidant, Hepatoprotective, and Anticancer Substance

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