Radiation-Induced Changes of microRNA Expression Profiles in Radiosensitive and Radioresistant Leukemia Cell Lines with Different Levels of Chromosome Abnormalities

Liamina, Daria; Sibirnyj, Wladimir; Khokhlova, Anna; Saenko, V. V.; Rastorgueva, Eugenia; Fomin, A. N.; Saenko, Yury

doi:10.3390/cancers9100136

Cited by 8 publications

(3 citation statements)

References 44 publications

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“…In a study conducted by Su et al, hsa_circ_001059 and hsa_circ_000167 levels were shown to be dysregulated in radioresistant ESCC cell line as compared to the parental cell line (Su et al, 2016). The analysis showed that circRNA_001059 could sponge to multiple miRNAs including miR-30c-1, miR-30c-2, miR-122, miR-139-3p, miR-339-5p, and miR-1912. In support of this finding, miR-30 and miR-122 were found to be dysregulated in chemoresistant prostate cancer and miR-30 in radiosensitive leukemia cells (Ni et al, 2017;Liamina et al, 2017). These dysregulated circRNAs were mapped to their target genes and were found to be mainly involved in the Wnt signaling pathway and the PI3K/Akt pathway.…”

Section: Circrna Effects Radiotherapy Receptivity Via Wnt Pathwaymentioning

confidence: 58%

Circular RNAs: Potential Regulators of Treatment Resistance in Human Cancers

Jeyaraman¹,

Eam²,

Mutalib³

et al. 2020

Front. Genet.

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Circular RNAs (circRNAs) which were once considered as "junk" are now in the spotlight as a potential player in regulating human diseases, especially cancer. With the development of high throughput technologies in recent years, the full potential of circRNAs is being uncovered. CircRNAs possess some unique characteristics and advantageous properties that could benefit medical research and clinical applications. CircRNAs are stable with covalently closed loops that are resistant to ribonucleases, have disease stage-specific expressions and are selectively abundant in different types of tissues. Interestingly, the presence of circRNAs in different types of treatment resistance in human cancers was recently observed with the involvement of a few key pathways. The activation of certain pathways by circRNAs may give new insights to treatment resistance management. The potential usage of circRNAs from this aspect is very much in its infancy stage and has not been fully validated. This mini-review attempts to highlight the possible role of circRNAs as regulators of treatment resistance in human cancers based on its intersection molecules and cancer-related regulatory networks.

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Section: Circrna Effects Radiotherapy Receptivity Via Wnt Pathwaymentioning

confidence: 58%

Circular RNAs: Potential Regulators of Treatment Resistance in Human Cancers

Jeyaraman¹,

Eam²,

Mutalib³

et al. 2020

Front. Genet.

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“…In tumor cells, cell cycle arrest and the suppression of apoptosis are important factors associated with the development of radiation resistance (51). Increasing evidence suggests that miRNAs are involved in these processes (56,57). Thus, CCK-8 assays were performed in the present study to evaluate the role of miR-30a-3p in transfected EC9706 and EC109 cells irradiated with various radiation doses.…”

Section: Discussionmentioning

confidence: 99%

MiRNA‑30a‑3p inhibits metastasis and enhances radiosensitivity in esophageal carcinoma by targeting insulin‑like growth factor 1 receptor

et al. 2019

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It has been demonstrated that microRNAs (miRNAs) serve important roles in various biological processes, such as tumorigenesis. In the present study, the role of miR-30a-3p in the pathogenesis of esophageal carcinoma (EC) was investigated. Reverse transcription-quantitative polymerase chain reaction was performed to determine the levels of miR-30a-3p expression in EC tissues and cell lines. Then, the effects of miR-30a-3p on the migration, invasion and radiosensitivity of EC cells were investigated using scratch-wound, Transwell and radiosensitivity assays, respectively. A dual-luciferase reporter assay was performed to determine potential interactions between miR-30a-3p and the 3′-untranslated region (3′-UTR) of insulin-like growth factor 1 receptor (IGF-1R). The results demonstrated that the levels of miR-30a-3p expression in EC tissues and cell lines were significantly decreased compared with those in paired healthy tissues and a human esophageal epithelial cell line. Upregulation of miR-30a-3p expression significantly suppressed migration, invasion and epithelial-mesenchymal transition (EMT), and enhanced radiosensitivity in EC cells. Analysis of luciferase activity demonstrated that miR-30a-3p interacted with the 3′-UTR of IGF-1R, and knockdown of IGF-1R induced similar effects on the migration, invasion, EMT and radiosensitivity of EC cells. The results indicated that miR-30a-3p suppressed metastasis and enhanced the radiosensitivity of EC cells via downregulation IGF-1R, suggesting that miR-30a-3p may be a potential therapeutic target in the treatment of EC.

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“…On the other hand, only a few miRNAs are functionally characterized in neutrophils (17). In HL-60 cells, miRNA expression changes during cell differentiation (18)(19)(20) and after radiation (21) or resveratrol treatment (22). Indeed, multiple miRNAs regulate HL-60 growth, differentiation and survival in vitro (20,(23)(24)(25)(26)(27)(28)(29)(30)(31).…”

Section: Introductionmentioning

confidence: 99%

Inducible overexpression of zebrafishmicroRNA-722suppresses chemotaxis of human neutrophil like cells

Hsu

Liu

Brasseale

et al. 2019

Preprint

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Neutrophil migration is essential for battling against infections but also drives chronic inflammation. Since primary neutrophils are terminally differentiated and not genetically tractable, leukemia cells such as HL-60 are differentiated into neutrophil-like cells to study mechanisms underlying neutrophil migration. However, constitutive overexpression or inhibition in this cell line does not allow the characterization of the genes that affect the differentiation process. Here we apply the tet-on system to induce the expression of zebrafish miR-722 in differentiated HL-60. Overexpression of miR-722 reduced the mRNA level of genes in the chemotaxis and inflammation pathways, including RAC2. Consistently, cell migration is significantly inhibited upon induced miR-722 overexpression. Together, miR-722 is an evolutionarily conserved suppressor for neutrophil migration and signaling in zebrafish and human. providing the Odyssey imaging system (LI-COR).

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Radiation-Induced Changes of microRNA Expression Profiles in Radiosensitive and Radioresistant Leukemia Cell Lines with Different Levels of Chromosome Abnormalities

Cited by 8 publications

References 44 publications

Circular RNAs: Potential Regulators of Treatment Resistance in Human Cancers

Circular RNAs: Potential Regulators of Treatment Resistance in Human Cancers

MiRNA‑30a‑3p inhibits metastasis and enhances radiosensitivity in esophageal carcinoma by targeting insulin‑like growth factor 1 receptor

Inducible overexpression of zebrafishmicroRNA-722suppresses chemotaxis of human neutrophil like cells

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