Radiation-induced glioblastoma signaling cascade regulates viability, apoptosis and differentiation of neural stem cells (NSC)

Ivanov, Vladimir N.; Hei, Tom K.

doi:10.1007/s10495-014-1040-x

Cited by 31 publications

(27 citation statements)

References 49 publications

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“…This cell cycle delay provides time for DNA to repair and allows recovery of cells in contrast to apoptosis induction at higher dose rates [5]. The measurement of cell viability plays a fundamental role in predicting of tissue/cell response to different types of radiation [18,19]. While these data are preliminary, our laboratory is further exploring the differences in the modes of cell death in the in vitro HPBL model following irradiation with protons and photons.…”

Section: Resultsmentioning

confidence: 99%

Effects of 60 MeV Protons and 250 kV X-Rays on Cell Viability

et al. 2016

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Particle radiotherapy such as the one using proton beams, provides a successful treatment approach in many cancer types. However, the cellular and molecular mechanisms by which proton irradiation induces cell death, particularly in a human peripheral blood lymphocyte model has not been examined in detail. Comparative studies of the biological effects, such as cell death, of particle therapy versus conventional X-rays treatment are of utmost importance. Here, we compared the viability of human peripheral blood lymphocyte following in vitro irradiation with protons (therapeutic 60 MeV proton beam) and photon beam (250 kV, X-rays), by applying separate doses within the range of 0.3-4.0 Gy. Cell viability was assessed 1 and 4 h after irradiation with protons and X-rays by the FITC-Annexin V labelling procedure (Apoptotic & Necrotic & Healthy Cells Quantification Kit, Biotium). Results showed that irradiation with both radiation types reduced the number of viable cells in a dose-dependent manner, as assessed as a function of the duration of post-irradiation time. Protons proved more fatal to the cells treated than X-ray photons. This demonstrates a difference in cell viability after irradiation with protons and photons in a human peripheral blood lymphocyte model.

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Section: Resultsmentioning

confidence: 99%

Effects of 60 MeV Protons and 250 kV X-Rays on Cell Viability

et al. 2016

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“…Fig.1). We recently observed high levels of apoptosis induced by such treatment in glioblastoma cells [51]. A combination of BMS345541 and LY294002 (40 μM) induced pronounced levels of apoptotic and total death in NCS 48 h after treatment.…”

Section: Resultsmentioning

confidence: 99%

“…5D and E). Increasing expression of CD133 in HHMSX cells is reminiscent of the upregulation of CD133 expression in spheroids of U97MG glioblastoma [51, 59]. …”

Section: Resultsmentioning

confidence: 99%

Regulation of viability, differentiation and death of human melanoma cells carrying neural stem cell biomarkers: a possibility for neural trans-differentiation

Ivanov

Hei

2015

Apoptosis

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During embryonic development, melanoblasts, the precursors of melanocytes, emerge from a subpopulation of the neural crest stem cells and migrate to colonize skin. Melanomas arise during melanoblast differentiation into melanocytes and from young proliferating melanocytes through somatic mutagenesis and epigenetic regulations. In the present study, we used several human melanoma cell lines from the sequential phases of melanoma development (radial growth phase, vertical growth phase and metastatic phase) to compare: i) the frequency and efficiency of the induction of cell death via apoptosis and necroptosis; ii) the presence of neural and cancer stem cell biomarkers as well as death receptors, DR5 and FAS, in both adherent and spheroid cultures of melanoma cells; iii) anti-apoptotic effects of the endogenous production of cytokines and iv) the ability of melanoma cells to perform neural trans-differentiation. We demonstrated that programed necrosis or necroptosis, could be induced in two metastatic melanoma lines, FEMX and OM431, while the mitochondrial pathway of apoptosis was prevalent in a vast majority of melanoma lines. All melanoma lines used in the current study expressed substantial levels of pluripotency markers, SOX2 and NANOG. There was a trend for increasing expression of Nestin, an early neuroprogenitor marker, during melanoma progression. Most of the melanoma lines, including WM35, FEMX and A375, can grow as a spheroid culture in serum-free media with supplements. It was possible to induce neural trans-differentiation of 1205Lu and OM431 melanoma cells in serum-free media supplemented with insulin. This was confirmed by the expression of neuronal markers, Doublecortin and β3-Tubulin, by significant growth of neurites and by the negative regulation of this process by a dominant-negative Rac1N17. These results suggest a relative plasticity of differentiated melanoma cells and a possibility for their neural trans-differentiation without the necessity for preliminary dedifferentiation.

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“…In malignant gliomas, RT induces the secretion of interleukins 6 and 8 (IL-6, IL-8) and growth factors, such as transforming growth factor β (TGF-β) and vascular endothelial growth factor (VEGF) that contribute to increased cell motility, thus affecting tumor invasion and migration [55–58]. Secretion of matrix metalloproteinases (MMPs) following irradiation has also been shown to enhance invasion and migration of brain tumors [59, 60].…”

Section: Mechanisms Of Radiation-induced Alterations Of Tumor Cellmentioning

confidence: 99%

Effects of radiation on metastasis and tumor cell migration

Vilalta

Rafat

Graves

2016

Cell. Mol. Life Sci.

119

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It is well known that tumor cells migrate from the primary lesion to distant sites to form metastases and that these lesions limit patient outcome in a majority of cases. However the extent to which radiation influences this process and to which migration in turn alters radiation response remains controversial. There are preclinical and clinical reports showing that focal radiotherapy can both increase the development of distant metastasis, as well as that it can induce the regression of established metastases through the abscopal effect. More recently, preclinical studies have suggested that radiation can attract migrating tumor cells and may thereby facilitate tumor recurrence. In this review, we summarize these phenomena and their potential mechanisms of action, and evaluate their significance for modern radiation therapy strategies.

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Radiation-induced glioblastoma signaling cascade regulates viability, apoptosis and differentiation of neural stem cells (NSC)

Cited by 31 publications

References 49 publications

Effects of 60 MeV Protons and 250 kV X-Rays on Cell Viability

Effects of 60 MeV Protons and 250 kV X-Rays on Cell Viability

Regulation of viability, differentiation and death of human melanoma cells carrying neural stem cell biomarkers: a possibility for neural trans-differentiation

Effects of radiation on metastasis and tumor cell migration

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