Radiation-induced miR-208a increases the proliferation and radioresistance by targeting p21 in human lung cancer cells

Tang, Yiting; Cui, Yayun; Li, Zengpeng; Jiao, Zhuqing; Zhang, Yong; He, Yan; Chen, Guangxia; Zhou, Qunyan; Wang, Wenjie; Zhou, Xifa; Luo, Judong; Zhang, Shuyu

doi:10.1186/s13046-016-0285-3

Cited by 155 publications

(133 citation statements)

References 47 publications

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“…Apoptosis is an important mechanism for the regulation of sensitivity to radiation [9, 27]. We found that overexpression of miR-19b-3p or knockout of TNFAIP3 decreased apoptosis rates of NPC cell lines after irradiation.…”

Section: Discussionmentioning

confidence: 99%

“…Using bioinformatic analyses, we found that both miR-19b-3p and TNFAIP3 have a common apoptotic pathway—the NF-κB signaling pathway. Ionizing radiation is known to cause DNA DSBs and leads to lethal injury [9, 28]. IR-induced DSBs trigger a DNA damage response with NF-κB activation as the antiapoptotic factor in most cell types [29–32].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies indicate that miRNAs directly affect radioresistance by interfering with specific pathways [4], including sensing DNA damage [5], repair of DNA double-strand breaks (DSBs) [6], cell cycle checkpoint activation [7], formation of reactive oxygen species [8] and apoptosis [9], and autophagy [10]. miR-19b-3p belongs to both the miR-17-92 and miR-106-363 clusters, which play significant roles in proliferation and cell survival [11, 12].…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-19b-3p regulates nasopharyngeal carcinoma radiosensitivity by targeting TNFAIP3/NF-κB axis

Huang

et al. 2016

J Exp Clin Cancer Res

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BackgroundNasopharyngeal carcinoma (NPC) is among the most common squamous cell carcinoma in South China and Southeast Asia. Radiotherapy is the primary treatment for NPC. However, radioresistance acts as a significant factor that limits the efficacy of radiotherapy for NPC patients. Growing evidence supports that microRNAs (miRNAs) play an important role in radiation response.MethodsReal-time quantitative PCR was used to analyze the expression of miR-19b-3p in NPC cell lines and NP69. miR-19b-3p expression profiles in NPC tissues were obtained from the Gene Expression Omnibus database. The effect of miR-19b-3p on radiosensitivity was evaluated by cell viability assays, colony formation assays and in vivo experiment. Apoptosis and cell cycle were examined by flow cytometry. Luciferase reporter assay was used to assess the target genes of miR-19b-3p. Expression of target proteins and downstream molecules were analyzed by Western blot.ResultsmiR-19b-3p was upregulated in NPC and served as an independent predictor for reduced patient survival. Radioresponse assays showed that miR-19b-3p overexpression resulted in decreased sensitivity to irradiation, whereas miR-19b-3p downregulation resulted in increased sensitivity to irradiation in vitro. Moreover, miR-19b-3p decreased the sensitivity of NPC cells to irradiation in vivo. Luciferase reporter assay confirmed that TNFAIP3 was a direct target gene of miR-19b-3p. Knockdown of TNFAIP3 reduced sensitivity to irradiation, whereas upregulation of TNFAIP3 expression reversed the inhibitory effects of miR-19b-3p on NPC cell radiosensitivity. Mechanistically, we found that miR-19b-3p increased NPC cell radioresistance by activating the TNFAIP3/ NF-κB axis.ConclusionsmiR-19b-3p contributes to the radioresistance of NPC by activating the TNFAIP3/ NF-κB axis. miR-19b-3p is a determinant of NPC radioresponse and may serve as a potential therapeutic target in NPC treatment.Electronic supplementary materialThe online version of this article (doi:10.1186/s13046-016-0465-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MicroRNA-19b-3p regulates nasopharyngeal carcinoma radiosensitivity by targeting TNFAIP3/NF-κB axis

Huang

et al. 2016

J Exp Clin Cancer Res

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“…Accumulating evidence has indicated that miRNAs are important regulatory factors involved in various biological processes, including cell differentiation, growth, metastasis and apoptosis (8,9). Aberrant expression of miRNAs has been identified in numerous cancers, including lung and breast cancer and hepatocellular carcinoma (10)(11)(12)(13)(14)(15). Numerous miRNAs may be regarded as potential and novel biomarkers for the diagnosis, therapy and prognosis of numerous types of cancer (16,17).…”

Section: Introductionmentioning

confidence: 99%

miR‑20b promotes cellular proliferation and migration by directly regulating phosphatase and tensin homolog in prostate cancer

Guo

Xiao

et al. 2017

Oncol Lett

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Abstract. MicroRNAs are small non-coding RNAs, which are critical regulators of carcinogenesis and tumor progression. Previous studies have identified that microRNA-20b (miR-20b) acts as an oncogene in numerous cancers. However, the role of miR-20b in prostate cancer remains unclear. The present study aimed to investigate the expression of miR-20b in prostate cancer and to examine whether modulating miR-20b expression impacts prostate cancer cellular proliferation and migration. It was revealed that miR-20b was strongly expressed in prostate cancer tissues compared with adjacent normal prostate tissues (P<0.05). Knockdown of miR-20b expression by miR-20b inhibitor inhibited VCaP and PC-3 cell growth and migration. Through bioinformatics analysis, phosphatase and tensin homolog (PTEN) was predicted as a target gene of miR-20b in prostate cancer cells, which was validated by dual-luciferase reporter assay and western blot analysis. In addition, restoration of PTEN expression levels did not affect endogenous miR-20b expression in prostate cancer cells. In conclusion, the present study indicated that miR-20b promotes cellular proliferation and migration by directly regulating PTEN in prostate cancer.

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“…The mechanisms of programmed cell death, apoptosis and autophagy, are launched in normal cells when cell damage repair, especially, DNA damage, is impossible. Programmed cell death induced in stress exposure is not only associated with the extent of DNA damage, but involves a great number of other signaling mechanisms and depends on their interaction [3,4]. The functioning of signaling and metabolic mechanisms is associated with gene expression regulation at the transcriptional and post-transcriptional levels.…”

mentioning

confidence: 99%

The Influence of microRNA on Signaling Pathways Activity in Radiosensitive and Radioresistant Cancer Cell Lines after Radiation Exposure

Масленникова¹,

Khokhlova²,

Pogodina³

et al. 2017

Sovrem Tehnol Med

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The aim of the investigation was to study microRNA expression and its influence on signaling pathways activity in radioresistant and radiosensitive cell lines exposed to radiation.Materials and Methods. Radioresistant K562 cell line and radiosensitive HL-60 and Raji cell lines were used in the study. Cell survival was estimated after exposure to 4 Gy gamma radiation. MicroRNA composition was studied 1, 4 and 24 h after radiation exposure using massively parallel sequencing method. Bioinformatics analysis was performed using GenXpro and PANTHER database.Results. After single 4 Gy radiation exposure, the number of cells with the signs of necrosis increased several times in radiosensitive cell lines as compared to control samples. MicroRNA hsa-miR-590-3p was found in each studied cell line at every stage of the experiment. The most significant differences between radioresistant and radiosensitive cell lines were observed in dynamics of microRNA influence on Integrin signaling pathway and General transcription by RNA polymerase I pathway.Conclusion. MicroRNA hsa-miR-590-3p dynamics and expression level were found to correlate with cancer cell radiosensitivity. Its influence on radioresistant and radiosensitive cell lines was different: in radioresistant K562 cell line, the inhibitory effect of microRNA on Integrin signaling pathway was reduced and this effect on General transcription by RNA polymerase I pathway was increased.

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Radiation-induced miR-208a increases the proliferation and radioresistance by targeting p21 in human lung cancer cells

Cited by 155 publications

References 47 publications

MicroRNA-19b-3p regulates nasopharyngeal carcinoma radiosensitivity by targeting TNFAIP3/NF-κB axis

MicroRNA-19b-3p regulates nasopharyngeal carcinoma radiosensitivity by targeting TNFAIP3/NF-κB axis

miR‑20b promotes cellular proliferation and migration by directly regulating phosphatase and tensin homolog in prostate cancer

The Influence of microRNA on Signaling Pathways Activity in Radiosensitive and Radioresistant Cancer Cell Lines after Radiation Exposure

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