Radiation-induced translocations with reduced Haynaldia villosa chromatin at the Pm21 locus for powdery mildew resistance in wheat

Chen, Peidu; You, Chunfang; Hu, Yin Gang; Chen, Shengwei; Zhou, Bo; Cao, Aizhong; Wang, Xiue

doi:10.1007/s11032-012-9804-x

Cited by 82 publications

(49 citation statements)

References 17 publications

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“…Thus cross‐species breeding by reintroducing disease resistance genes from wheat relatives is valuable for breeding more resilient cultivars. Rye and Dasypyrum villosum , close and wild relatives of common wheat, respectively, have been used for transmitting a variety of resistance genes, especially against powdery mildew and rust fungi (Graybosch, 2001; Chen et al , 2013; Li et al , 2018). For example, genes for resistance to powdery mildew ( Pm8 and Pm17 ) and rust ( Sr31 , Lr26 , and Yr9 ) on chromosome 1RS of rye have been successfully introduced into commercial wheat cultivars (Jiang et al , 1994; Kim et al , 2004).…”

Section: Prospects In Current Resistance Breedingmentioning

confidence: 99%

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

et al. 2020

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Powdery mildew (Blumeria graminis f. sp. tritici) results in serious economic loss in wheat production. Exploration of plant resistance to wheat powdery mildew over several decades has led to the discovery of a wealth of resistance genes and quantitative trait loci (QTLs). We have provided a comprehensive summary of over 200 powdery mildew genes (permanently and temporarily designated genes) and QTLs reported in common bread wheat. This highlights the diverse and rich resistance sources that exist across all 21 chromosomes. To manage different data for breeders, here we also present a bridged mapping result from previously reported powdery mildew resistance genes and QTLs with the application of a published integrated wheat map. This will provide important insights to empower further breeding of powdery mildew resistant wheat via marker‐assisted selection (MAS).

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Section: Prospects In Current Resistance Breedingmentioning

confidence: 99%

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

et al. 2020

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“…The acquirement of breeding-usable materials through alien gene introgression is very difficult. Many researchers extensively explored strategies to develop small segmental translocation lines with less negative effects using irradiation and Ph1 system, such as an improved scheme of chromosome engineering for efficient elimination of a large amount of goatgrass (Aegilops speltoides) chromatin surrounding Sr39, a reduced H. Villosa containing chromatin the Pm21 locus for powdery mildew resistance in wheat, and the creation of wheat-rye terminal and intercalary chromosomal translocations (Bie et al 2007;Chen et al 2013;Lukaszewski 2000;Mukai et al 1993;Niu et al 2011). Generally, translocations with smaller alien segments are genetically more stable and less likely to have deleterious effects (Faris et al 2008).…”

Section: Wheat-a Cristatum 6p Intercalary Translocation Line Pubing3mentioning

confidence: 99%

Introgression of Agropyron cristatum 6P chromosome segment into common wheat for enhanced thousand-grain weight and spike length

Zhang

Liu

et al. 2015

Theor Appl Genet

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This study explored the genetic constitutions of wheat-Agropyron cristatum 6P chromosomal translocation and determined the effects of 6P intercalary chromosome segment on thousand-grain weight and spike length in wheat. Crop wild relatives provide rich genetic resources for wheat improvement. Introduction of alien genes from Agropyron cristatum into common wheat can broaden its genetic diversity. In this study, radiation-induced wheat-A. cristatum translocation line Pubing3035 derived from the offspring of wheat-A. cristatum 6P chromosomes addition line was identified and analyzed using genomic in situ hybridization (GISH), dual-color fluorescence in situ hybridization (FISH), and molecular markers. GISH analysis revealed that Pubing3035 was a Ti1AS-6PL-1AS·1AL intercalary translocation. The breakpoint was pinpointed to locate near the centromeric region on the short arm of wheat chromosome 1A based on a constructed F2 linkage map and it was flanked by markers SSR12 and SSR263. The genotypic data, combined with the phenotypes, indicated that A. cristatum 6P chromosomal segment played an important role in regulating the thousand-grain weight and spike length. On average, the thousand-grain weight and spike length in translocation individuals were approximately 2.5 g higher and 0.7 cm longer than those in non-translocation individuals in F2 and BC1F1 populations. The clusters of quantitative trait loci for thousand-grain weight, spike length, and spikelet density contributed by 6P chromosome segment were mapped between A. cristatum unique marker Agc7155 and wheat marker SSR263, which, respectively, explained phenotypic variance of 24.96, 12.38 and 17.20 % with an LOD of 10.63, 4.89 and 5.59. Overall, the translocation Pubing3035 had a positive effect on the yield of wheat, which laid the foundation for the localization of A. cristatum excellent genes and made itself a promising and valuable germplasm for wheat improvement.

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“…This newly designed marker (STPK-A) will play an effective role in the identification and characterization of Pm21 resistance gene in a wheat population. In previous studies, several PCR-based markers associated with Pm21 gene were reported Song et al, 2009;Chen et al, 2013;He et al, 2013;Bie et al, 2015) as mentioned in Table 2, but many of them have shortcomings and complications for breeding, such as unpredictability, dominance rather than co-dominance and low visual determination. Among them, WS-1 marker was potentially associated with Pm21 gene as described by Zheng et al, (2014).…”

Section: Discussionmentioning

confidence: 99%

Development and Verification of Wheat Germplasm Containing Pm21

Shah¹

2018

IJAB

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Powdery mildew, a devastating disease that affects wheat, is caused by a Blumeria graminis f. sp. tritici. Powdery mildew is the obstacle in the cultivation of high yielding cultivars especially for wet climatic regions throughout the world. Developing new cultivar with high disease resistance genes is a commercially feasible and environmentally safe approach to reduce yield loss. In order to develop a marker associated with Pm21 gene, a pair of primers (STPK-F and STPK-R) was designed according to the sequence of a serine/threonine kinase gene (accession: HQ864471.1) using Primer Premier 5.0 software. To screen and characterize Pm21 gene, we used 37 recombinant inbred lines (RIL) of 02P67//Aizao-781/Yangmai158 along with five susceptible cultivars. Initially, these RIL were screened at both seedling and adult plant stages with B. graminis f. sp. tritici for visible scoring of powdery mildew resistant lines. It was observed that only a particular (Pm21) gene governs the resistance to disease in all the 43 set of genotypes.

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Radiation-induced translocations with reduced Haynaldia villosa chromatin at the Pm21 locus for powdery mildew resistance in wheat

Cited by 82 publications

References 17 publications

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

Introgression of Agropyron cristatum 6P chromosome segment into common wheat for enhanced thousand-grain weight and spike length

Development and Verification of Wheat Germplasm Containing Pm21

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