Radiation-inducible radioprotective exopolysaccharides of <i>Bacillus siamensis</i> CV5 from irradiated roots of <i>Cistanche violacea</i> to decrease free radical damage produced by ionizing radiation

Guesmi, Sihem; Chouchane, Habib; Neifar, Mohamed; Hosni, F.; Chérif, Ameur; Sghaier, Haïtham

doi:10.1080/09553002.2019.1649501

Cited by 14 publications

(15 citation statements)

References 48 publications

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“…EPS (IC 50 , 0.38 mg/ml) [40]. It has been reported that the antioxidant activity of polysaccharides is closely linked to their physiochemical properties and functional groups, mainly the uronic acid and sulfate contents [3,21,44,45]. In the present study, CS-hEPS contained a significant proportion of uronic acid and sulfate.…”

Section: Discussionsupporting

confidence: 50%

“…e precipitate in the centrifuging tube was rinsed with distilled water, filtered, and then dried at 45°C using rotavap. Proteins were removed from CS-hEPS as described in our previous study [21]. For in vivo activities, lyophilized CS-hEPS were further purified using a Sephadex G-100 column (2.6 cm × 50 cm), and the column was eluted with 0.01 M NaCl solution at a flow rate of 1 mL/min [22].…”

Section: Cs-heps Production and Purificationmentioning

confidence: 99%

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Carboxymethylated Sulfated Heteroexopolysaccharide from a Haloarchaeal Strain as Potential Biomolecule for Harmless Adjuvant Therapy in Cancer Treatment

Chouchane¹,

Najjari

Chérif³

et al. 2020

Journal of Chemistry

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is study explored the possible use of a microbial carboxymethylated sulfated heteroexopolysaccharide (CS-hEPS) as a potential anticancer agent. e investigation was carried out through antioxidant, antifatigue, and antiproliferative activities. Antioxidant potential including scavenging DPPH and hydroxyl radical activities and reducing power was evaluated. Antifatigue activity was determined by assessing the endurance of mice using the forced swimming test. Following 30 days of CS-hEPS oral treatment at different doses, biochemical parameters related to fatigue such as lactic dehydrogenase (LDH), serum urea nitrogen (SUN), and hepatic glycogen (HG) contents were measured. Antitumor activities were investigated against human cancer liver and myelogenous leukemia cells. Results showed that CS-hEPS possesses notable antioxidant, antifatigue, and antitumor effects. CS-hEPS significantly inhibited the proliferation of leukemia (86.6 ± 0.32%) and cancer liver (58.6 ± 0.43%) cells. CS-hEPS are promising natural antioxidant, antifatigue, and antitumor harmless adjuvant materials that could be applied in human cancer therapy.

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Section: Discussionsupporting

confidence: 50%

Section: Cs-heps Production and Purificationmentioning

confidence: 99%

Carboxymethylated Sulfated Heteroexopolysaccharide from a Haloarchaeal Strain as Potential Biomolecule for Harmless Adjuvant Therapy in Cancer Treatment

Chouchane¹,

Najjari

Chérif³

et al. 2020

Journal of Chemistry

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“…Gamma radiation was reported to induce EPS in Bacillus sp. it is thought to act as an antioxidant that protects bacterial cells from ionizing radiation and thus is considered a radio-protector (Guesmi et al, 2019). Besides EPS increase, total protein increased as well upon exposure to gamma radiation, this is attributed to an increase in protein synthesis, a response that is unanimous to all organisms exposed to environmental stress.…”

Section: Discussionmentioning

confidence: 99%

“…Gamma radiation is a form of ionizing radiation, it can induce a myriad of responses to cells, and the responses range from interacting with different cellular molecules, altering gene expression, damaging nucleic acids, or causing cell death (Shuryak, 2019). But at low doses, it can induce the production of exopolysaccharides in bacterial cells (Guesmi et al, 2019) and therefore, increase biofilm formation. From this standpoint, modifying bacterial cell membrane using gamma radiation may be considered the key step that contributes to both direct and mediated electron transfer and therefore, enhancing MFC performance.…”

Section: Introductionmentioning

confidence: 99%

Modification of bacterial cell membrane to accelerate decolorization of textile wastewater effluent using microbial fuel cells: role of gamma radiation

Selim

Maghrawy

Fathy

et al. 2020

Journal of Radiation Research and Applied Sciences

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The aim of the present work was to increase bacterial adhesion on anode via inducing membrane modifications to enhance textile wastewater treatment in Microbial Fuel Cell (MFC). Real textile wastewater was used in mediator-less MFCs for bacterial enrichment. The enriched bacteria were pre-treated by exposure to 1 KGy gamma radiation and were tested in MFC setup. Bacterial cell membrane permeability and cell membrane charges were measured using noninvasive dielectric spectroscopy measurements. The results show that pre-treatment using gamma radiation resulted in biofilm formation and increased cell permeability and exopolysaccharide production; this was reflected in both MFC performance (average voltage 554.67 mV) and decolorization (96.42%) as compared to 392.77 mV and 60.76% decolorization for non-treated cells. At the end of MFC operation, cytotoxicity test was performed for treated wastewater using a dermal cell line, the results obtained show a decrease in toxicity from 24.8 to 0 (v/v%) when cells were exposed to gamma radiation. Fourier-transform infrared (FTIR) spectroscopy showed an increase in exopolysaccharides in bacterial consortium exposed to increasing doses of gamma radiation suggesting that gamma radiation increased exopolysaccharide production, providing transient media for electron transfer and contributing to accelerating MFC performance. Modification of bacterial membrane prior to MFC operation can be considered highly effective as a pre-treatment tool that accelerates MFC performance.

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“…At present, EPSs are used as active components of some biological drugs, dietary supplements, microbial flocculants, emulsifiers, and antioxidants. They are also used as agents to combat aging, radiation exposure, and tumor proliferation [5][6][7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from Halomonas desertis G11: Biochemical and Genomic Insights

Chouchane¹,

Boutiti²,

Ouertani³

et al. 2021

Polymers

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In this work, a native exopolysaccharide (nEPS) produced by Halomonas desertis G11 isolated from a Tunisian extreme environment was modified by gamma irradiation. Characterization as well as the antioxidant and antitumor activities of nEPS and its gamma-irradiated derivatives (iEPSs) were comparatively evaluated. In vitro and in vivo antioxidant potentials were determined by using different methods and through different antioxidant enzymes. The antitumor activity was checked against a human colon cancer cell line. Analyses of the complete genome sequence were carried out to identify genes implicated in the production of nEPS. Thus, the genomic biosynthesis pathway and the export mechanism of nEPS were proposed. Analyses of irradiation data showed that iEPSs acquired new functional groups, lower molecular weights, and gained significantly (p < 0.05) higher antioxidant and antitumor abilities compared with nEPS. These findings provide a basis for using iEPSs as novel pharmaceutical agents for human therapies.

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Radiation-inducible radioprotective exopolysaccharides of Bacillus siamensis CV5 from irradiated roots of Cistanche violacea to decrease free radical damage produced by ionizing radiation

Cited by 14 publications

References 48 publications

Carboxymethylated Sulfated Heteroexopolysaccharide from a Haloarchaeal Strain as Potential Biomolecule for Harmless Adjuvant Therapy in Cancer Treatment

Carboxymethylated Sulfated Heteroexopolysaccharide from a Haloarchaeal Strain as Potential Biomolecule for Harmless Adjuvant Therapy in Cancer Treatment

Modification of bacterial cell membrane to accelerate decolorization of textile wastewater effluent using microbial fuel cells: role of gamma radiation

Effect of Gamma Irradiation on Enhanced Biological Activities of Exopolysaccharide from Halomonas desertis G11: Biochemical and Genomic Insights

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