Radiation Intensity and Quality Affect Indoor Acclimation of Blueberry Transplants

Abstract

The objectives were to characterize and compare shrinkage (i.e., transplant loss) and growth of tissue-cultured blueberry (Vaccinium corymbosum) transplants acclimated in greenhouses or indoors under 1) different photosynthetic photon flux densities (PPFDs) (Expt. 1); or 2) spectral changes over time using broad-spectrum white (W; 400 to 700 nm) light-emitting diodes (LEDs) without or with red or far-red (FR) radiation (Expt. 2). In Expt. 1, ‘Emerald’ and ‘Snowchaser’ transplants were acclimated for 8 weeks under PPFDs of 35, 70, 105, or 140 ± 5 µmol·m‒2·s‒1 provided by W LED fixtures for 20 h·d−1. In another treatment, PPFD was increased over time by moving transplants from treatment compartments providing 70 to 140 µmol·m‒2·s‒1 at the end of week 4. Transplants were also acclimated in either a research or a commercial greenhouse (RGH or CGH, respectively). Shrinkage was unaffected by PPFD, but all transplants acclimated indoors had lower shrinkage (≤4%) than those in the greenhouse (15% and 17% in RGH and CGH, respectively), and generally produced more shoot and root biomass, regardless of PPFD. Growth responses to increasing PPFD were linear in most cases, although treatment effects after finishing were generally not significant among PPFD treatments. In Expt. 2, ‘Emerald’ transplants were acclimated for 8 weeks under constant W, W + red (WR), or W + FR (WFR) radiation, all of which provided a PPFD of 70 ± 2 μmol·m−2·s−1 for 20 h·d−1. At the end of week 4, a group of transplants from WR and WFR were moved to treatment compartments with W (WRW or WFRW, respectively) or from W to a research greenhouse (WGH), where another group of transplants were also acclimated for 8 weeks (GH). Shrinkage of transplants acclimated indoors was also low in Expt. 2, ranging from 1% to 4%. In contrast, shrinkage of transplants acclimated in GH or under WGH was 37% or 14%, respectively. Growth of indoor-acclimated transplants was generally greater than that in GH or under WGH. Although growth responses were generally similar indoors, plants acclimated under WFR had a higher root dry mass (DM) and longer roots compared with GH and WGH.

Peculiarities of ex vitro growing completion of bog cranberry (Vaccinium oxycoccos L.) with a closed root system

“…Освещённость также играет немаловажную роль в развитии надземной системы вовремя доращивания ex vitro растений. По некоторым исследованиям, реакция роста на увеличение интенсивности света во время акклиматизации линейна, что свидетельствует о том, что более высокая PPFD может дополнительно увеличить рост и развитие саженцев [74].…”