Radiation protection of DNA by ferulic acid under in vitro and in vivo conditions

Maurya, Dharmendra Kumar; Salvi, Veena P.; Nair, Cherupally Krishnan Krishnan

doi:10.1007/s11010-005-0170-4

Cited by 76 publications

(48 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…SF was also reported to be effective in preventing bone loss in ovariectomized rats (Sassa et al, 2003), promoting bone remodeling, and leading to a predominantly osteoblastic phase. It was also found to inhibit radiation-induced DNA strand breaks and enhance the survival of mice (Maurya et al, 2005;Maurya and Devasagayam, 2013). Therefore, it is interesting to investigate the potential advantages of SF on the femoral head osteonecrosis.…”

Section: Introductionmentioning

confidence: 99%

Effects of sodium ferulate on preventing steroid-induced femoral head osteonecrosis in rabbits

Tian

Dang

Wang

et al. 2013

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Abstract:The aim of this study is to investigate the effects and possible mechanisms of sodium ferulate (SF) on anti-apoptosis in steroid-induced femoral head osteonecrosis in rabbits. Japanese white rabbits were randomly divided into three groups (control group, treatment group, and model group), each with 24 rabbits. The model and treatment groups were first injected with an intravenous dose of horse serum, 10 ml/kg, three weeks later with an intravenous dose of 7.5 ml/kg, and two weeks later with an intramuscular dose of methylprednisolone, 45 mg/kg, three times in order to establish rabbit models of osteonecrosis. Concurrently, the treatment group was injected with intravenous doses of SF 20 mg/kg for two weeks, once per day. Three time points, Weeks 2, 4, and 8, were selected after modeling was completed. Osteonecrosis was verified by histopathology with haematoxylin-eosin (HE) staining. The apoptosis rate of osteonecrosis was observed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The apoptosis expressions of caspase-3 and Bcl-2 were analyzed by immunohistochemistry and Western blot. The rabbit models of osteonecrosis were successfully established and observed by HE staining. SF was effective in intervening in apoptosis and decreasing the apoptosis rate in femoral head necrosis by the immunohistochemistry and TUNEL assay (P<0.01). Western blot analysis indicated that there were statistical significances in the protein levels of caspase-3 and Bcl-2 (P<0.01). SF has a protective effect by reducing the incidence of early steroid-induced femoral head necrosis in rabbits, effectively intervening in apoptosis through decreasing caspase-3 expression and up-regulating Bcl-2 expression.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of sodium ferulate on preventing steroid-induced femoral head osteonecrosis in rabbits

Tian

Dang

Wang

et al. 2013

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

show abstract

“…Moreover, FA is a strong scavenger of free radicals and it has been approved in certain countries as food additive to prevent lipid peroxidation [13]. FA possesses radioprotective abilities and reduces ionizing radiation induced damages to DNA and membranes in biological systems [14]. …”

Section: Introductionmentioning

confidence: 99%

Amelioration of Doxorubicin Induced Cardiotoxicity in Tumor Bearing Mice by Ferulic Acid: a Mechanistic Study at Cellular and Biochemical Level

Divakaran¹,

Nai²

2012

IJTT

View full text Add to dashboard Cite

Doxorubicin (DOX) is one of the most effective anticancer therapeutic but its use is limited by cardiotoxicity.The generation of reactive oxygen species (ROS) and mitochondrial dysfunction have been implicated in DOX-induced cardiotoxicity. Cardiotoxicity was induced in tumor bearing mice by a single dose of DOX (25mg/kg, i.p). Ferulic acid (FA) (100 mg/kg p.o and 200 mg/kg p.o) was administered one hour after DOX administration. The administration of FA significantly protected the myocardium from the toxic effects of DOX by reducing the levels of serum marker enzymes like CK and LDH and other serum enzymes SGOT and SGPT, which were elevated during DOX induced cardiomyopathy. The level of HDL was also significantly increased in FA administered groups compared to DOX control. FA protected the cardiac tissues, whereas it potentiated the anticancer efficacy of DOX in tumor tissues as evident from different antioxidant enzyme levels and the extent of lipid peroxidation. The histopathological observations also supported these results. FA effectively protected cellular DNA in heart tissue preferentially, without offering any protection to the DNA in tumour tissues as evidenced from the comet assay. These results suggest that ferulic acid has a protective effect against cardiotoxicity induced by DOX and it may, therefore improve the chemotherapeutic index of DOX.

show abstract

“…8) FA, an effective radioprotector during radiotherapy, stimulates hematopoiesis, immunity and DNA repair in irradiated mice. 9) It is widely used in preventing cardiovascular disease, and restores endothelial function by enhancing the bioavailability of basal and stimulated nitric oxide (NO) in spontaneously hypertensive rat aortas. 10) Further, it inhibits endothelial cell proliferation through the NO down-regulating extracellular signal regulated kinase 1/2 (ERK1/2) pathway, 11) and inhibits vascular smooth muscle cell proliferation by inactivation of mitogen-activated protein kinases (MAPKs) and modulation of cell cycle proteins.…”

mentioning

confidence: 99%

Ferulic Acid Attenuates Adhesion Molecule Expression in Gamma-Radiated Human Umbilical Vascular Endothelial Cells

Qian

Wang

et al. 2010

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Radiation induces an important inflammatory response in the irradiated organs, characterized by leukocyte infiltration and vascular changes. Since adhesion molecules play an important role in facilitating the immune response at the inflammation sites, interfering with the expression of these molecules may be an important therapeutic target of radiation induced inflammation. Many adhesion molecules such as intercellular cell adhesion molecule 1 (ICAM-1), and vascular cell adhesion molecule 1 (VCAM-1) have been identified in radiation. Ferulic acid (FA), an effective radioprotector during radiotherapy, is widely used in endothelium protection. The present study examined the effect of FA on the induction of adhesion molecules by gamma-radiation and the mechanisms of its effect in gamma-irradiated human umbilical vein endothelial cells (HUVECs). HUVECs were pretreated for 18 h with FA and then exposed to 10 Gy radiation. The result of cell adhesion assay showed FA inhibited radiation-induced U937 adhesion to HUVECs. FA prevented induction of ICAM-1 and VCAM-1 expression in a concentration-dependent manner after stimulation with radiation at the level of mRNA and protein. Inhibitors of the extracellular signal regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) pathways were used to determine which pathway was involved in FA action; the result showed that the inhibitory effect of FA on adhesion molecule expression was mediated by the blockade of JNK. FA appears to be a potential therapeutic agent for treating various inflammatory disorders including radiation induced inflammation.

show abstract

Radiation protection of DNA by ferulic acid under in vitro and in vivo conditions

Cited by 76 publications

References 24 publications

Effects of sodium ferulate on preventing steroid-induced femoral head osteonecrosis in rabbits

Effects of sodium ferulate on preventing steroid-induced femoral head osteonecrosis in rabbits

Amelioration of Doxorubicin Induced Cardiotoxicity in Tumor Bearing Mice by Ferulic Acid: a Mechanistic Study at Cellular and Biochemical Level

Ferulic Acid Attenuates Adhesion Molecule Expression in Gamma-Radiated Human Umbilical Vascular Endothelial Cells

Contact Info

Product

Resources

About