Radiation-sensitive mutants of T4D I. T4y: A new radiation-sensitive mutant: Effect of the mutation on radiation survival, growth and recombination

Boyle, John M; Symonds, N.

doi:10.1016/0027-5107(69)90060-8

Cited by 127 publications

(28 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mutation of the uvsY gene results in T4 phage that displays UV and ␥-ray sensitivity, low burst size, and low levels of recombination (29,30). UvsY interacts with UvsX protein and with gp32 (31,32).…”

mentioning

confidence: 99%

Escherichia coli RecO protein anneals ssDNA complexed with its cognate ssDNA-binding protein: A common step in genetic recombination

Kantake

Madiraju²,

Sugiyama

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

125

118

View full text Add to dashboard Cite

mentioning

confidence: 99%

Escherichia coli RecO protein anneals ssDNA complexed with its cognate ssDNA-binding protein: A common step in genetic recombination

Kantake

Madiraju²,

Sugiyama

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

125

118

View full text Add to dashboard Cite

“…Overnight cultures were diluted at least 30-fold into fresh medium. Cells were collected by centrifugation when at densities of about 2 x 108/ml and suspended to the same densities in phage buffer (5). UV irradiation of cell suspensions was at a fluence rate of 0.10 or 0.18 W/m2.…”

Section: Methodsmentioning

confidence: 99%

Specificity of mutation by UV light and delayed photoreversal in umuC-defective Escherichia coli K-12: a targeting intermediate at pyrimidine dimers

Bockrath¹,

Ruiz-Rubio²,

Bridges³

1987

J Bacteriol

View full text Add to dashboard Cite

Prototrophic mutants produced by UV light in Escherichia coli K-12 strains with argE3(0c) and hisG4(0c) defects are distinguished as backmutations and specffic nonsense suppressor mutations. In strains carrying a umuC defect, mutants are not produced unless irradiated cells are incubated and then exposed to photoreversing light (delayed photoreversal mutagenesis). The mutants thus produced are found to be specifically suppressor mutations and not backmutations. The suppressor mutations are primarily glutamine tRNA ochre suppressor mutations, which have been attributed previously to mutation targeted at T = C pyrimidine dimers.In a lexASi recA441 strain, where the SOS mutagenesis functions are constitutive, targeting at dimers is confirmed by demonstrating that the induction of glutamine tRNA suppressor mutations is susceptible to photoreversal. In the same strain induction of backmutations is not susceptible to photoreversal. Thus delayed photoreversal mutagenesis produces suppressor mutations that can be targeted at pyrimidine dimers and does not produce backmutations that are not targeted at pyrimidine dimers. This correlation supports the idea that delayed photoreversal mutagenesis in umuC defective cells reflects a mutation process arrested at a targeting pyrimidine dimer photoproduct, which is the immediate cause of both the alteration in DNA sequence and the obstruction (unless repaired) to mutation fixation and ultimate expression.

show abstract

“…For phage T4, this process requires the products of T4 genes 58, w, x, and y , as yet characterized enzymatically, as well as all the normal phage replication proteins (8, 86,[99][100][101][102][103]. For phage T4, this process requires the products of T4 genes 58, w, x, and y , as yet characterized enzymatically, as well as all the normal phage replication proteins (8, 86,[99][100][101][102][103].…”

Section: Re Pli Cation-dependent Re Pair Recombinationmentioning

confidence: 99%

Molecular and Genetic Recombination of Bacteriophage T4

Broker¹,

Doermann²

1975

Annu. Rev. Genet.

View full text Add to dashboard Cite

Most genetic characteristics of T4 recombination (the effects of chromosomal termini on recombination, heterozygosity, high negative interference, base mismatch repair, polarized segregation, and the stimulation or depression of recombination in response to phage mutations or external perturbations) can be expressed adequately in terms of our present understanding of the molecular events of T4 infection cycles. T4 DNA replication begins and ends with linear chromosomes and does not require a circular intermediate. Replication is bi-directional, possibly from multiple origins. Two phases of replication are distinguishable: (a) an early mode during which about 20 progeny copies of infecting chromosomes are made prior to (b) a recombination-dependent mode during which progeny molecules associate to form covalently joined linear concatemers. Further replication is generally arrested if concatemerization is prevented. T4 DNA recombination depends on the production of single-stranded gaps and termini. If replication is inhibited, the single-stranded regions are produced by deoxyribonuclease activities. In contrast, during partial replication of damaged chromosomes, during slow replication when enzymes or subtrates are limited, and during normal replication, they are formed by strand-displacement DNA polymerization. As a rule, any agents or perturbations that cause an accumulation of single-stranded regions stimulate recombination, whereas efficient repair of such regions depresses it. Both the preservation and pairing of single-stranded regions are facilitated by the gene-32 single-stranded DNA binding protein. Covalent repair of strand interruptions between recombined DNA segments requires polymerases or nucleases as well as ligases.

show abstract

Radiation-sensitive mutants of T4D I. T4y: A new radiation-sensitive mutant: Effect of the mutation on radiation survival, growth and recombination

Cited by 127 publications

References 14 publications

Escherichia coli RecO protein anneals ssDNA complexed with its cognate ssDNA-binding protein: A common step in genetic recombination

Escherichia coli RecO protein anneals ssDNA complexed with its cognate ssDNA-binding protein: A common step in genetic recombination

Specificity of mutation by UV light and delayed photoreversal in umuC-defective Escherichia coli K-12: a targeting intermediate at pyrimidine dimers

Molecular and Genetic Recombination of Bacteriophage T4

Contact Info

Product

Resources

About