Radical polymerization-crosslinking method for improving extracellular matrix stability in bioprosthetic heart valves with reduced potential for calcification and inflammatory response

Guo, Guanhua; Jin, Linhe; Jin, Wanyu; Chen, Liang; Lei, Yang; Wang, Yunbing

doi:10.1016/j.actbio.2018.10.017

Cited by 74 publications

(67 citation statements)

References 67 publications

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“…Extracting free dialdehydes and using α‐amino‐oleic acid or amino compounds like L‐lysine are possible ways to reduce bioprosthetic tissue calcification. But other factors can also cause adverse calcification, such as inadequate stabilization of ECM components . Therefore, we did not perform anticalcification treatments because our purpose was not merely to reduce calcification, but to improve the overall properties of BHVs, including reendothelialization potential, thrombogenesis, and calcification.…”

Section: Discussionmentioning

confidence: 99%

Hybrid Pericardium with VEGF‐Loaded Hyaluronic Acid Hydrogel Coating to Improve the Biological Properties of Bioprosthetic Heart Valves

Lei

Deng

Tang

et al. 2019

Macromolecular Bioscience

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Bioprosthetic heart valves (BHVs) used in the clinic are mostly fixed by glutaraldehyde and the lack of endothelialization is a major problem for glutaraldehyde‐fixed pericardia. Hyaluronic acid is a major glycosaminoglycan that exists in native heart valves. Coupled with its inherent biocompatibility, it may enhance endothelial adhesion and proliferation when associated with vascular endothelial growth factor (VEGF). In this study, an optimized system is developed to improve the endothelialization of glutaraldehyde‐fixed pericardium. A hybrid pericardium with VEGF‐loaded hyaluronic acid hydrogel coating is developed by the crosslinking of 1,4‐butanediol diglycidyl ether. The adhesion and growth potential of human umbilical vein endothelial cells (HUVECs) on pericardia, platelet adhesion, and calcification by an in vivo rat subdermal implantation model are investigated. The results show improved HUVEC adhesion and proliferation, less platelet adhesion, and less calcification for hybrid pericardium by introducing the coating of VEGF‐loaded hyaluronic acid hydrogel. Thus, the coating of VEGF‐loaded hyaluronic acid hydrogel on pericardium is a promising approach to obtain bioprosthetic valves for clinical applications with increased endothelialization and antithrombotic and anticalcification properties.

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Section: Discussionmentioning

confidence: 99%

Hybrid Pericardium with VEGF‐Loaded Hyaluronic Acid Hydrogel Coating to Improve the Biological Properties of Bioprosthetic Heart Valves

Lei

Deng

Tang

et al. 2019

Macromolecular Bioscience

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“…3,28,29 Guo, Jin et al. 3,30 successfully modified MA and glycidyl methacrylate (GMA) monomers on porcine pericardium by radical polymerization crosslinking, thus greatly improving the biocompatibility and anticalcification performance of BHVs, demonstrating the effectiveness of such method. Porcine aortic valve (PAV) is also a commonly used xenogenic tissue material for BHVs.…”

Section: Introductionmentioning

confidence: 99%

Crosslinking porcine aortic valve by radical polymerization for the preparation of BHVs with improved cytocompatibility, mild immune response, and reduced calcification

Yang

Yao

et al. 2021

J Biomater Appl

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Over one million artificial heart valve transplantations are performed each year due to valvular stenosis or regurgitation. Among them, bioprosthetic heart valves (BHVs) are increasingly being used because of the absence of the need for lifelong anticoagulation. Almost all of the commercial BHVs are treated with Glutaraldehyde (GLUT). As GLUT-treated BHVs are prone to calcification and structural degradation, their durability is greatly reduced with a service life of only 12–15 years. The physiological structure and mechanical properties of the porcine aortic valve (PAV) are closer to that of a human heart valve, so in this study, PAV is used as the model to explore the comprehensive properties of the prepared BHVs by radical polymerization crosslinking method. We found that PAV treated by radical polymerization crosslinking method showed similar ECM stability and biaxial mechanical properties with GLUT-treated PAV. However, radical polymerization crosslinked PAV exhibited better cytocompatibility and endothelialization potential in vitro cell experiment as better anticalcification potential and reduced immune response than GLUT-treated PAV through subcutaneous animal experiments in rats. To conclude, a novel crosslinking method of non-glutaraldehyde fixation of xenogeneic tissues for the preparation of BHVs is expected.

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“…In addition, more efficient cross-linking methods should be developed. However, the grafts were utilized in the reconstruction of connections between the pulmonary artery and right ventricle, which remained a low anti-calcification potential, and provided reliable mechanical properties as compared to GA treatment (Guo et al, 2018). TEVG, tissue-engineered vascular graft cross-linking agent and is widely used in biomaterial production, such as bioprosthetic valves, vessel prosthesis, and many other tissueengineered materials.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

“…The tensile strength of the cross-linked grafts was significantly increased, and the hemodynamic performance was much better than non-treated grafts (Lu et al, 2010;Lü et al, 2009). However, the grafts were utilized in the recon- ties as compared to GA treatment (Guo et al, 2018). These novel cross-linking methods showed great application potential in biomaterial production, and they may be feasible in TEVG manufacturing, especially in decellularized ECM grafts.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

“…Yunbing Wang et al developed a non‐GA cross‐linking method based on radical polymerization cross‐linking (RPC) to improve extracellular matrix stability, prevent calcification, and reduce the inflammatory response. They demonstrated that high‐density RPC treatment remarkably enhanced the stability of collagen and elastin of porcine pericardium, improved its endothelialization and anti‐calcification potential, and provided reliable mechanical properties as compared to GA treatment (Guo et al, ). These novel cross‐linking methods showed great application potential in biomaterial production, and they may be feasible in TEVG manufacturing, especially in decellularized ECM grafts.…”

Section: Unanswered Questions and The Future Of Tevgmentioning

confidence: 99%

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Strategies in cell‐free tissue‐engineered vascular grafts

Yuan

Chen

Liu

et al. 2019

J Biomedical Materials Res

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Cardiovascular diseases (CVD) remain the leading cause of morbidity and mortality in the world, among which coronary artery diseases (CAD) are the most common type of CVD. Coronary artery bypass grafting (CABG) using autologous vein and artery grafts is the typical surgical intervention for CAD patients. However, for patients whose autologous grafts are not available, there are no appropriate substitutes for vascular grafts. Investigation of tissue-engineered vascular graft (TEVG) has persisted over decades with significant advancement, utilizing different types of biomaterials.In the past two decades, a great number of studies based on cell-seeding strategies were reported. However, limitations of cell-based strategies made clinical application difficult. With the understanding of stem cells and tissue remodeling process, strategies without cell-seeding emerged as potential methods to achieve in situ regeneration.A cell-free graft may recruit host cells and guide their participation in vascular remodeling. The grafts modified by bio-active molecules showed good results in promoting in situ regeneration and exhibited potential to make the vascular grafts off-the-shelf.In this review, the strategies for cell-free TEVG manufacturing were discussed, including the materials for fabricating TEVGs, the methods of functionalization to promote in situ regeneration, the challenges researchers faced in TEVG investigation, and finally the prospects in TEVG design.

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Radical polymerization-crosslinking method for improving extracellular matrix stability in bioprosthetic heart valves with reduced potential for calcification and inflammatory response

Cited by 74 publications

References 67 publications

Hybrid Pericardium with VEGF‐Loaded Hyaluronic Acid Hydrogel Coating to Improve the Biological Properties of Bioprosthetic Heart Valves

Hybrid Pericardium with VEGF‐Loaded Hyaluronic Acid Hydrogel Coating to Improve the Biological Properties of Bioprosthetic Heart Valves

Crosslinking porcine aortic valve by radical polymerization for the preparation of BHVs with improved cytocompatibility, mild immune response, and reduced calcification

Strategies in cell‐free tissue‐engineered vascular grafts

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