Radical SAM enzymes involved in the biosynthesis of purine-based natural products

Bandarian, Vahe

doi:10.1016/j.bbapap.2012.07.014

Cited by 23 publications

(15 citation statements)

References 69 publications

(78 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of particular note is the presence of the radical S-adenosyl-l-methionine (SAM) protein (ID: BC346_05545) in this area. The radical SAM protein is a group of iron-sulfur containing proteins that exploit the reactivity of the high energy intermediate, the 5’-deoxyadenosyl radical, which is produced by reductive cleavage of SAM, to carry-out complex radical-mediated transformations [ 30 ]. In microorganisms, the activity of the radical SAM protein is very important for bacterial adaption to environmental niches and survival [ 31 , 32 ].…”

Section: Resultsmentioning

confidence: 99%

Comparative Genome Analysis of a Pathogenic Erysipelothrix rhusiopathiae Isolate WH13013 from Pig Reveals Potential Genes Involve in Bacterial Adaptions and Pathogenesis

Yang

Zhu

Peng

et al. 2020

Veterinary Sciences

View full text Add to dashboard Cite

Erysipelothrix rhusiopathiae is a common pathogen responsible for pig erysipelas. However, the molecular basis for the pathogenesis of E. rhusiopathiae remains to be elucidated. In this study, the complete genome sequence of the E. rhusiopathiae strain WH13013, a pathogenic isolate from a diseased pig, was generated using a combined strategy of PacBio RSII and Illumina sequencing technologies. The strategy finally generated a single circular chromosome of approximately 1.78 Mb in size for the complete genome of WH13013, with an average GC content of 36.49%. The genome of WH13013 encoded 1633 predicted proteins, 55 tRNAs, as well as 15 rRNAs. It contained four genomic islands and several resistance-associated genes were identified within these islands. Phylogenetic analysis revealed that WH13013 was close to many other sequenced E. rhusiopathiae virulent strains. The comprehensive comparative analysis of eight E. rhusiopathiae virulent strains, including WH13013, identified a total of 1184 core genes. A large proportion (approximately 75.31%) of these core genes participated in nutrition and energy uptake and metabolism as well as the other bioactivities that are necessary for bacterial survival and adaption. The core genes also contained those encoding proteins participating in the biosynthesis and/or the components of the proposed virulence factors of E. rhusiopathiae, including the capsule (cpsA, cpsB, cpsC), neuraminidase (nanH), hyaluronidase (hylA, hylB, hylC), and surface proteins (spaA, rspA, rspB). The obtaining of the complete genome sequence of this virulent strain, WH13013, and this comprehensive comparative genome analysis will help in further studies of the genetic basis of the pathogenesis of E. rhusiopathiae.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative Genome Analysis of a Pathogenic Erysipelothrix rhusiopathiae Isolate WH13013 from Pig Reveals Potential Genes Involve in Bacterial Adaptions and Pathogenesis

Yang

Zhu

Peng

et al. 2020

Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…QueE along with other enzymes required for the initial steps of queuosine biosynthesis (QueC, QueD, QueF) were first identified in Bacillus subtilis by comparative genomics combined with experimental validation in Acinetobacter baylyi 31 . B. subtilis QueE was subsequently studied in detail and shown to be a CDG synthase 22 23 32 33 ( Supplementary Fig. 8 ).…”

Section: Discussionmentioning

confidence: 99%

“…The filamentous cells have a continuous cytoplasm and intact FtsZ rings, suggesting a block downstream of Z-ring formation in the cell division pathway. From a suppressor screen, we determined that filamentation depends on QueE, an enzyme involved in the biosynthesis of a hyper-modified guanosine (queuosine) found in certain tRNAs 22 23 . We further find that PhoP regulates queE transcription, and that increased expression of QueE inhibits cell division.…”

mentioning

confidence: 99%

Antimicrobial peptides trigger a division block in Escherichia coli through stimulation of a signalling system

et al. 2016

View full text Add to dashboard Cite

Antimicrobial peptides are an important component of the molecular arsenal employed by hosts against bacteria. Many bacteria in turn possess pathways that provide protection against these compounds. In Escherichia coli and related bacteria, the PhoQ/PhoP signalling system is a key regulator of this antimicrobial peptide defence. Here we show that treating E. coli with sublethal concentrations of antimicrobial peptides causes cells to filament, and that this division block is controlled by the PhoQ/PhoP system. The filamentation results from increased expression of QueE, an enzyme that is part of a tRNA modification pathway but that, as we show here, also affects cell division. We also find that a functional YFP–QueE fusion localizes to the division septum in filamentous cells, suggesting QueE blocks septation through interaction with the divisome. Regulation of septation by PhoQ/PhoP may protect cells from antimicrobial peptide-induced stress or other conditions associated with high-level stimulation of this signalling system.

show abstract

“…80 In particular, radical SAM enzymes post-translationally modify many RiPPs through epimerization of L-to D-amino acids, 19,81 excision of tyramine to form a-keto moieties, 82 and formation of intramolecular crosslinks including strained cyclophane macrocycles. 83 Radical SAMs also play a role in the biosynthesis of hypermodied tRNA bases 84 and nucleoside-based natural products through C-C bond extension at C5 0 of ribose rings to connect nucleosides to structurally diverse functional groups. 85 A number of other enzyme classes not covered in detail here also were predicted to have remarkable across-EC-class reaction diversity.…”

Section: Hotbeds For Enzyme Discoverymentioning

confidence: 99%

A roadmap for metagenomic enzyme discovery

2021

View full text Add to dashboard Cite

show abstract

Radical SAM enzymes involved in the biosynthesis of purine-based natural products

Cited by 23 publications

References 69 publications

Comparative Genome Analysis of a Pathogenic Erysipelothrix rhusiopathiae Isolate WH13013 from Pig Reveals Potential Genes Involve in Bacterial Adaptions and Pathogenesis

Comparative Genome Analysis of a Pathogenic Erysipelothrix rhusiopathiae Isolate WH13013 from Pig Reveals Potential Genes Involve in Bacterial Adaptions and Pathogenesis

Antimicrobial peptides trigger a division block in Escherichia coli through stimulation of a signalling system

A roadmap for metagenomic enzyme discovery

Contact Info

Product

Resources

About