Radical scavenging assay and determination Flavonoid and Phenolic total of extract and Fractions of <i>Raghu</i> bark (<i>Dracontomelon dao</i> (Blanco) Merr)

Yamin, Yamin; Ruslin, Ruslin; Sartinah, Ari; Ihsan, Sunandar; Kasmawati, Henny; Suryani, Suryani; Andriyani, Rina; Asma, -; Adjeng, Andi Nafisah Tendri; Arba, Muhammad

doi:10.5958/0974-360x.2020.00420.5

Cited by 10 publications

(6 citation statements)

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“…22 The data in Table 2 shows that the ethyl acetate fraction has a stronger antioxidant activity compared to methanol extract, chloroform fraction, n-hexane fraction, and water fraction with values of 6.338 ± 0.005 µg/mL, 6.846 ± 0.005 µg/mL, 7.086 ± 0.083 µg / mL, 8.354 ± 0.077 µg / mL and 9.291 ± 0.036 µg/mL, respectively. This is consistent with previous studies which showed that the ethyl acetate fraction was stronger, including the ethyl acetate fraction of Raghu (Dracontomelon dao (Blanco) Merr) stem bark, 23 ethyl acetate fraction of rambutan peels two cultivars: cultivar Aceh and Binjai, 24 ethyl acetate fraction of senggani (Melastoma candidum D. Don) leaves, fraksi ethyl acetate of Langsat (Lansium domesticum Coor.) Seeds 25 and the ethyl acetate fraction of the Oroxilum indicum Linn bark.…”

Section: Antiradical Test Dpph Radical Sacavenging Activitysupporting

confidence: 93%

Determination of Antiradical Activity, Total Phenolic and Flavonoid Contents of Kamena-mena Kamena-mena (Clerodendrum paniculatum. L) Leaves

2021

TJNPR

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Kamena-mena (Clerodendrum paniculatum. L) is a plant that has the potential as a source of natural antioxidants. Traditionally, Kamenamena is used to treat rheumatism, ulcers, neuralgia, inflammation, wounds, antipyretics, eye diseases. 7 Several studies have reported that C. paniculatum leaves have pharmacological activity, such as anti-bacterial activity, 8 antioxidant activity, 9 anti-inflammatory activity in vitro and in vivo, 10 and antioxidant activity in vitro and antiinflammatory in vivo. 11 This study aims to evaluate the antiradical activity of extract and fractions of Kamena-mena (Clerodendrum paniculatum Linn) leaf using the DPPH (2, 2-diphenyl-1-picryhidrazyl) and ABTS (2, 2'azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) method and determination of phenolic and flavonoid contents.

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Section: Antiradical Test Dpph Radical Sacavenging Activitysupporting

confidence: 93%

Determination of Antiradical Activity, Total Phenolic and Flavonoid Contents of Kamena-mena Kamena-mena (Clerodendrum paniculatum. L) Leaves

2021

TJNPR

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“…Linn (Trang et al, 2014), Polygala sabulosa, Cyathea phalerata (Brighente et al, 2008), as well as the stem bark from Dracontomelon dao(Blanco) Merr (Yamin et al, 2020). The DPPH is a free radical widely used to examine radical scavenging activity of plant extracts (Jamuna et al, 2012), pure compounds, food ingredients, and others (Koleva et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

Determination of Antiradical Activity, Total Phenolic, and Total Flavonoid Contents of Extracts and Fractions of Langsat (Lansium domesticum Coor.) Seeds

et al. 2020

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Lansium domesticum Coor. is a fruit species from the Meliaceae family, which is a tropical plant native to Southeast Asia. Local citizens call it langsat, longkong, or duku and have used it as traditional medicine. The seeds of L. domesticum are used as a fever medicine, its bark is used to treat scorpion sting, and its leaves are used to repel mosquitoes. Because of its various uses, it is necessary to explore the antiradical potential of L. domesticum seeds. This study aims to determine the antiradical potential of L. domesticum seeds extract and fractions by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and to discover compounds that act as antiradical. Lansium domesticum seed powder was macerated with methanol, and then the extract was concentrated using a rotary evaporator and fractionated by n-hexane and ethyl acetate. The antiradical assay was conducted on extract and fractions by using DPPH radicals. Phenolic and flavonoid contents from extract and fractions were also tested. The ethyl acetate fraction obtained strong antiradical potential with an IC50 value of 8.938 � 0.031 �g/mL. Total phenolic and flavonoid contents of ethyl acetate fraction were higher with values ??of 58.25 � 0.501 mgGAE/g sample and 75.123 � 0.175 mgQE/g sample, respectively. Correlation of phenolic and flavonoid contents, which inhibited radicals had R2 values ??of 0.9182 and 0.7658. Ethyl acetate fraction of L. domesticum seeds had very strong antiradical activity. Further isolation is expected to be conducted to discover which compounds are the most responsible as antiradical.

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“…PE and WPI contained more flavonoids. Flavonoid compounds also have potential medicinal value, and investigations have shown that ingestion of flavonoids can reduce the incidence of various non-communicable diseases, and some flavonoids also show strong physiological activity in vivo and in vitro [ 40 , 41 ]. Thus, tea flowers with color might be a useful resource for processing as drinks for health promotion or used in substance extraction.…”

Section: Discussionmentioning

confidence: 99%

“…The phenylalanine metabolic pathway can be divided into two branches: the phenylalanine metabolic pathway and flavonoid metabolic pathway; and flavonoid biosynthesis is an important downstream branch of phenyl C metabolism [ 52 ]. Naringin plays a central role in the metabolic pathway that forms other isoflavones, flavones, and flavonols during its metabolism [ 41 ]. Flavonoid scaffolds also undergo a variety of tailoring reactions, such as glycosylation, methylation, and acylation, to form metabolites with different physicochemical and biological properties, which are catalyzed by flavonoid methyltransferase and flavonoyltransferase, respectively [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolite Profiling of External and Internal Petals in Three Different Colors of Tea Flowers (Camellia sinensis) Using Widely Targeted Metabolomics

Zhang

Zhou

et al. 2023

Metabolites

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The flower is the reproductive organ of the tea plant, while it is also processed into different kinds of products and thus of great significance to be utilized. In this study, the non-volatile secondary metabolites in the internal and external petals of white, white and pink, and pink tea flowers were studied using a widely targeted metabolomics method with ultra-high liquid chromatography–tandem mass spectrometry (UPLC-MS/MS). A total of 429 metabolites were identified, including 195 flavonoids, 121 phenolic acids, 40 alkaloids, 29 lignans and coumarins, 19 tannins, 17 terpenoids, and 8 other metabolites. The metabolites in the internal and external petals of different colored flowers showed great changes in flavonoids. Most flavonoids and all tannins in the internal petals were higher compared with the external petals. Some phenolic acids were more accumulated in the external petals, while others showed opposite trends. The pink tea flower contained more flavonoids, alkaloids, lignans, coumarins, terpenoids, and tannins compared with white tea flowers. In addition, cyanidin-3-O-glucoside was more accumulated in the external petals of the pink flower, indicating that anthocyanin may be the main reason for the color difference between the pink and white tea flower. The enriched metabolic pathways of different colored flowers were involved in flavonoid biosynthesis, glycine, serine and threonine metabolism, glycerophospholipid metabolism, and phenylpropanoid biosynthesis. The findings of this study broaden the current understanding of non-volatile compound changes in tea plants. It is also helpful to lay a theoretical foundation for integrated applications of tea flowers.

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Radical scavenging assay and determination Flavonoid and Phenolic total of extract and Fractions of Raghu bark (Dracontomelon dao (Blanco) Merr)

Cited by 10 publications

References 0 publications

Determination of Antiradical Activity, Total Phenolic and Flavonoid Contents of Kamena-mena Kamena-mena (Clerodendrum paniculatum. L) Leaves

Determination of Antiradical Activity, Total Phenolic and Flavonoid Contents of Kamena-mena Kamena-mena (Clerodendrum paniculatum. L) Leaves

Determination of Antiradical Activity, Total Phenolic, and Total Flavonoid Contents of Extracts and Fractions of Langsat (Lansium domesticum Coor.) Seeds

Metabolite Profiling of External and Internal Petals in Three Different Colors of Tea Flowers (Camellia sinensis) Using Widely Targeted Metabolomics

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