Radical thermal therapy against infection with decapod iridescent virus 1 (DIV1)

Guo, Xiaomeng; Qiu, Liu; Gao, Wen; Wang, Guo-Hao; Chen, Xing; Huang, Jie

doi:10.1016/j.aquaculture.2022.738636

Cited by 7 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…iDIV1 in shrimp P. vannamei can only be eliminated by the newly developed thermal therapy at 36°C for 2 weeks (Guo et al., 2022). This method can be considered a rapid biosecurity measure to remove iDIV1 from significant live genetic stocks to restore a DIV1‐free status.…”

Section: Resultsmentioning

confidence: 99%

“…The new real-time PCR developed in this study demonstrated a DSe (sensitivity, the proportion of samples from known infected reference animals that tested positive) and a DSp (specificity, the proportion of samples from known uninfected reference animals that tested negative) of 100% (Table 1). iDIV1 in shrimp P. vannamei can only be eliminated by the newly developed thermal therapy at 36°C for 2 weeks (Guo et al, 2022). This method can be considered a rapid biosecurity measure to remove iDIV1 from significant live genetic stocks to restore a DIV1-free status.…”

Section: Clinical Sample Testmentioning

confidence: 99%

See 1 more Smart Citation

Establishment of a real‐time PCR for the detection of decapod iridescent virus 1 (DIV1)

Guo,

Xing,

et al. 2024

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Clinical Sample Testmentioning

confidence: 99%

Establishment of a real‐time PCR for the detection of decapod iridescent virus 1 (DIV1)

Guo,

Xing,

et al. 2024

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

show abstract

“…X0: The time when growth curve reaches maximum growth rate, and the result was presented as average value ± standard error ( SE ). A two-sample t test of the following formula was used to analyze the significant differences between growth curves ( 57 ). …”

Section: Methodsmentioning

confidence: 99%

Conjugative Transfer of Acute Hepatopancreatic Necrosis Disease-Causing pVA1-Type Plasmid Is Mediated by a Novel Self-Encoded Type IV Secretion System

Wang

et al. 2022

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

“…Bar, 20 mm (A-F). Qiu et al 10.3389/fmars.2023.1114123 vannamei infected with DIV1 exhibit an evident reddish body (Qiu et al, 2017;Qiu et al, 2021;Guo et al, 2022). The clinical sign of reddish body have been reported in many cases of shrimp disease in China and other Asian countries, but the earlier etiology is thought to be mainly due to infection with V. parahaemolyticus or WSSV (Huang et al, 1995;Alapide-Tendencia and Dureza, 1997;Liu et al, 1999;Fan et al, 2006;Jayasree et al, 2006;Zheng et al, 2011;Felix et al, 2011;Cao et al, 2014).…”

Section: Figurementioning

confidence: 99%

Susceptibility of kuruma shrimp to the infection with Decapod iridescent virus 1

et al. 2023

Self Cite

View full text Add to dashboard Cite

Infection with Decapod iridescent virus 1 (iDIV1), an important emerging disease of shrimps and crabs, has been included in the Quarterly Aquatic Animal Disease Report (QAAD) by the Network of Aquaculture Centres in Asia-Pacific (NACA) and listed by the World Organization for Animal Health (WOAH). China has classified iDIV1 as a Class II animal pandemic disease. In the present study, to determine the susceptibility of Penaeus japonicus to Decapod iridescent virus 1 (DIV1), healthy kuruma shrimp were artificially infected with DIV1 (isolate SHIV 20141215) by per os (the pathway that mimics natural transmission) and intramuscular injection (invasive pathway). The infected P. japonicus showed clinical signs such as anorexia, retardation, evident reddish body, swollen and whitish lymphoid organs, and mortalities of almost 100%. Real-time PCR showed that all the challenged individuals by per os or intramuscular routes were DIV1-positive with an average virus load between 10(9.09 ± 0.58) and 10(8.94 ± 0.45) copies/μg-DNA, respectively. Histological examination revealed karyopyknosis, and eosinophilic inclusions and minute basophilic stains were combined in lymphoid organs, hematopoietic tissue and gills of diseased individuals. In addition, lymphoid organs showed disorganization of the tubule matrix. In situ DIG-labeling loop-mediated isothermal amplification (ISDL) also demonstrated the presence of DIV1 signals existed in lymphoid organs, hemopoietic tissue, gills, epithelial tissue, hepatopancreas and muscle. Ultrathin sections examined using transmission electron microscopy (TEM) revealed the presence of DIV1 virions, the virogenic stroma, and the nucleocapsid production process in infected cells. In addition, pathogen surveillance of cultured samples showed that the DIV1 detection rate of farmed P. japonicus samples from five coastal provinces in China was 5.3% (9/157) in 2022. The results mentioned above support that P. japonicus is a newly confirmed susceptible host for DIV1, enhancing the pathogen ecological understanding of pathogens and giving more support for developing DIV1 preventive and control strategies.

show abstract

Radical thermal therapy against infection with decapod iridescent virus 1 (DIV1)

Cited by 7 publications

References 33 publications

Establishment of a real‐time PCR for the detection of decapod iridescent virus 1 (DIV1)

Establishment of a real‐time PCR for the detection of decapod iridescent virus 1 (DIV1)

Conjugative Transfer of Acute Hepatopancreatic Necrosis Disease-Causing pVA1-Type Plasmid Is Mediated by a Novel Self-Encoded Type IV Secretion System

Susceptibility of kuruma shrimp to the infection with Decapod iridescent virus 1

Contact Info

Product

Resources

About