Aim: To report the diagnosis and successful endodontic treatment of maxillary first and second premolars with anatomical variations.Summary: Although the maxillary premolars usually have two canals, it may rarely have three and this third canal can easily be missed. Meticulous knowledge of tooth morphology, careful interpretation of angled radiographs, proper access cavity preparation and a detailed exploration of the interior of the tooth is needed to ensure a proper endodontic treatment. Higher magnification and illumination can be useful for access cavity preparation and to recognize and locate additional canals. This article reports two rare findings of three separate roots in a maxillary first premolar and a maxillary second premolar during root canal treatment.