Radio-lmmunoassay for Plasma Alpha Human Atrial Natriuretic Peptide: A Comparison of Direct and Pre-Extracted Methods

Richards, A. Mark; Tonolo, Giancarlo; McIntyre, Gordon D.; Leckie, Brenda J.; Robertson, J. I. A.

doi:10.1097/00004872-198704000-00015

Cited by 100 publications

(48 citation statements)

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“…Our inability to detect the increase in endogenous circulating ANF in response to a high sodium intake that has been described by others 12 ' 2I probably reflects the presence of cross-reacting substances in unextracted plasma. 22 Such substances make only a minor contribution to the very high levels of immunoreactive ANF measured during and immediately after infusion of the synthetic hormone. Urinary volume and electrolyte excretion patterns were also similar for patients on the two diets, but natriuresis and diuresis were significantly greater during the highest dose of ANF in the high salt compared with the low salt diet group.…”

Section: Effect Of Anf Infusion On Plasma Renin Activity and Aldosteronementioning

confidence: 99%

Antihypertensive and hypotensive effects of atrial natriuretic factor in men.

et al. 1987

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SUMMARY Synthetic atrial natriuretic factor (ANF) was administered in ascending doses (0.03, 0.20, 0.45 /Lig/kg/min) to eight mildly essential hypertensive men on high (200 mEq/day) or low (10 mEq/day) sodium diets. Responses of blood pressure, heart rate, urinary volume and electrolyte excretion, renin, and aldosterone were measured. For the entire group, ANF lowered blood pressure and increased heart rate during the 0.20 and 0.45 ju,g/kg/min infusions, and the antihypertensive effect of the peptide persisted for at least 2 hours after the infusions ended. Four patients (2 at 0.20 jtxg/kg/min and 2 at 0.45 ptg/kg/min) experienced sudden bradycardia and hypotension at the end of or shortly after completion of ANF infusion. Renal excretion of water, sodium, chloride, calcium, and phosphorus increased in a dose-dependent fashion in response to infused ANF. Patients on the 200 mEq/day sodium diet had greater increases in urinary volume (11.1 ± 2.8 vs 3.0 ±2.0 ml/min; p<0.05), sodium (870 ± 134 vs 303 ± 27 /xEq/min; p<0.05), and chloride (801 ± 135 vs 176 ± 75 juEq/min; p<0.02) compared with patients on the low sodium diet. The apparent direct suppressive effect of a 0.03 /xg/kg/min infusion of ANF on renin and aldosterone levels was overcome at higher doses by counterregulation provoked by the depressor action. Renin was slightly (-12%) suppressed during the 0.03 /xg/kg/min infusion of ANF but increased at the 0.20 ( + 50%) and 0.45 /ng/kg/min ( + 90%; p<0.03) rates. Aldosterone declined significantly during the 0.03 jug/kg/min infusion ( -4 5 % ; p<0.01) of ANF but not during the two higher dose infusions. There were no significant differences in renin and aldosterone responses for the 200 mEq/day and 10 mEq/day sodium diets. We conclude that the depressor action of 1-hour infusions of very high doses of synthetic ANF in essential hypertensive men is associated with a high risk of late hypotension. Dietary sodium restriction diminishes the peak natriuretic effect of infused ANF but does not significantly affect responses of blood pressure, heart rate, renin, or aldosterone. 8 Such a pharmacodynamic profile would be very attractive in an antihypertensive drug, and studies of the cardiovascular actions of ANF in humans have been the subject of several recent reports. Initial human studies using bolus injections (100 /ig) confirmed the vasodepressor action of the peptide 9 but also suggested that the effect on arterial pressure is attenuated in hypertension.

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Section: Effect Of Anf Infusion On Plasma Renin Activity and Aldosteronementioning

confidence: 99%

Antihypertensive and hypotensive effects of atrial natriuretic factor in men.

et al. 1987

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“…It has been reported that extraction of plasma before assay removes interference from platelets and also from an as yet unidentified plasma component that can be demonstrated by gel filtration. 7 Acidification of samples prior to extraction has also been reported to affect the values of estimates. 7 -8 Ten laboratories provided detailed information relating to the pH of assay samples (before extraction), and for these there was no consistent difference between estimates from the three laboratories in which the pH of samples extracted was 4 or less and the seven laboratories in which the pH was more than 7.…”

Section: Discussionmentioning

confidence: 99%

The international standard for atrial natriuretic factor. Calibration by an international collaborative study.

et al. 1988

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“…Following extraction of plasma on C-18 reverse phase columns (Sep-Pak; Waters Assoc., Milford, MA, USA), peptide was subsequently measured by established radioimmunoassay [10]. Inter-and intra-assay variation was consistently less than 10%.…”

Section: Methodsmentioning

confidence: 99%

High dose inhaled atrial natriuretic peptide is a bronchodilator in asthmatic subjects

Hulks¹,

Thomson²

1994

Eur Respir J

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Atrial natriuretic peptide (ANP) has been shown to be an effective bronchodilator when given intravenously, but its efficacy by inhalation has not been assessed.In the first part of the current study, six asthmatic subjects, mean (SEM) forced expiratory volume in one second (FEV 1 ) 2.09 (0.30) l, received 0.1 and 1 mg atrial natriuretic peptide by inhalation, and in the second study five subjects, FEV 1 1.92 (0.40) l, received 5 mg ANP by inhalation. ANP was given in a placebo-controlled, double blind, randomized manner, with measurement of FEV 1 over the following 60 min. Nebulized salbutamol was given at 60 min as a measure of the maximal bronchodilator response attainable by conventional therapy.No significant bronchodilator effect was seen following the 0.1 or 1 mg inhalation, although the latter produced a minimal transient elevation in peripheral atrial natriuretic peptide plasma levels. A bronchodilator effect was seen with the 5 mg dose, which produced ∆FEV 1 0.42 (0.09) l compared to 0.93 (0.13) l subsequently produced by salbutamol. This effect peaked at 5 min and was no different from placebo from 10 min onwards We conclude that atrial natriuretic peptide may produce significant bronchodilation when given by inhalation in high doses, and speculate that substances which generate cyclic guanosine monophosphate (cGMP) in airway smooth muscle warrant further investigation as potential bronchodilatory agents.

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Radio-lmmunoassay for Plasma Alpha Human Atrial Natriuretic Peptide: A Comparison of Direct and Pre-Extracted Methods

Cited by 100 publications

References 0 publications

Antihypertensive and hypotensive effects of atrial natriuretic factor in men.

Antihypertensive and hypotensive effects of atrial natriuretic factor in men.

The international standard for atrial natriuretic factor. Calibration by an international collaborative study.

High dose inhaled atrial natriuretic peptide is a bronchodilator in asthmatic subjects

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