Radio Receptor Assay of Serum Neuroleptic Levels in Psychiatric Patients

Abstract

The radioreceptor assay for the measurement of neuroleptic drugs in serum has been used to study the relationship between dose and serum level in schizophrenic patients receiving these drugs. The assay was found to be reproducible and capable of detecting neuroleptics in the sera of patients receiving a range of both oral and depot drugs, with the exception of trifluoperazine spansules. Linear correlations were obtained between daily dose and serum level for each drug both in individual patients on different doses and between patients on a stable dose. Extrapyramidal side effects were related to the serum neuroleptic level within, but not between, patients. The assay may be of use in clinical practice, including the assessment of compliance or poor response to neuroleptics.

Receptor Occupancy-based Analysis of the Contributions of Various Receptors to Antipsychotics-induced Weight Gain and Diabetes Mellitus

“…25) In addition, the AUCs of all the drugs dealt with in this study are proportional to the dose. 20,[26][27][28][29][30][31][32] The concentration of quetiapine (nM) was expressed as quetiapine base (not as quetiapine fumarate that contains two quetiapine bases in one molecule).…”

Determination of clozapine in human plasma by solid-phase extraction and liquid chromatography with amperometric detection

“…Several methods for assay of clozapine in plasma are reported in the literature; they include thin-layer chromatography [5,6], radioimmunoassay [7][8][9], gas chromatography [6,[10][11][12], and highperformance liquid chromatography [13,14]. These methods are often complicated and time-consuming, particularly sample pretreatment.…”

Stability of serum neuroleptic and prolactin concentrations during short- and long-term treatment of schizophrenic patients

“…A non-linear relationship in this dose range may be due in part to methods with high coefficients of variation (Lader 1980;Goldman et al 1981) or patient's non-compliance (Balant-Georgia et al 1984). Recently Krska et al (1986) described correlation between dose and serum neuroleptic activity estimated with the radioreceptor assay in patients taking haloperidol (r= 0.82), thioridazine (r= 0.78) and other neuroleptics. We observed in the present study in patients treated long term with high doses of thioridazine an apparent curvilinear relationship in that the serum neuroleptic activity did not rise above 420 NU when a dose of > 300 mg thioridazine/d was administered.…”