Radio-sensitivity of<i>in vivo</i>and<i>in vitro</i>cultures of banana cv. Basrai (AAA)

Karmarkar, V.M.; Kulkarni, V. M.; Suprasanna, Penna; Bapat, V. A.; Rao, P. S.

doi:10.1051/fruits:2001114

Cited by 15 publications

(11 citation statements)

References 5 publications

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“…The lethal dose (LD 50 ) was found to be 33 Gy based on the survival percentage, the estimation was carried out using linear regression analysis. Karmarkar et al 2001 had made similar observations in cv. Basrai (AAA) which corroborate our finding.In principle, the most effective doses for mutation induction are thought to be the gamma doses lower than the LD 50 .…”

Section: Radiosensitivity and Determination Of Lethal Dose (Ld 50 )supporting

confidence: 57%

“…This could be due to perturbations of hormones balance inside the explant and the activity of hormone synthesized and transported from tip to the site of action or exogenous supply of auxin used for rooting. The inhibition of rooting at higher gamma doses have been reported by several researchers (Karmarkar et al 2001, Wi et al 2007and Kiong et al 2008 who described that rise of radiation dose increases plants sensitivity to gamma rays effect and reduces the amount of endogenous growth regulators, particularly the auxin and cytokines. This would resulted breakdown, or lack of synthesis and or disruption of hormonal balance and enzymatic activities.…”

Section: Effects On Rootingmentioning

confidence: 79%

“…However, in the recent studies by Karmarkar et al (2001), who had made an attempt to induce mutation using in vitro individual shoot, the researcher had exposed in vitro individual shoot to gamma radiation followed by sub culturing on rooting medium without undertaking sub-culture processes to examine the post-irradiation rooting response in the laboratory condition. However, he could not go further and observe the explants response to gamma irradiation in the nursery stage.…”

Section: Introductionmentioning

confidence: 99%

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Effect of gamma irradiation on the morphological and physiological variation from In vitro individual shoot of banana cv. Tanduk (Musa spp.)

2018

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Inducing genetic and morphological variation through conventional method is very difficult. Therefore, mutation induction through in vitro technology brings numerous advantages over the conventional breeding. Thus, the individual shoots (1~2 cm) were irradiated with gamma rays (10~70 Gy). The result revealed that the explants treated with higher doses (40, 50, 60, and 70 Gy) showed deleterious effects of ionizing radiation. The highest survival rate among γ treated explants recorded was 71% in 10 Gy treatments while the lowest survivality was 15% in 70 Gy. Lethal dose 50% (LD 50) dose was found to be 33 Gy. In the in vitro condition, rooting reponse showed that increase in gamma irradiation dose resulted in the inhibition of root growth. Meanwhile, non-treated explants had the best rooting ability with the maximum number of root per explant (20) within a short period of time (6 days), with the highest root length of (15.1 cm). The longer period in rooting (12 days) and lowest number of root per explant (8) with shortest root length (10.1 cm) were recorded at 30 Gy treatment. The highest shoot length (13.6 cm) was observed at control treatment and the shortest shoot length (10.4 cm) was observed at 30 Gy. In the nursery, lowest leaf number (5) was observed at 30 Gy compared with other treatments. The highest chlorophyll content (49.8) was recorded at 10 Gy treated seedling. Irradiated explants with 10 Gy found to be superior over the control treatment and had positive effects in main growth parameters such as chlorophyll content.

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Section: Radiosensitivity and Determination Of Lethal Dose (Ld 50 )supporting

confidence: 57%

Section: Effects On Rootingmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

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Effect of gamma irradiation on the morphological and physiological variation from In vitro individual shoot of banana cv. Tanduk (Musa spp.)

2018

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“…Nevertheless, Ferid et al [29] reported decline in shoot multiplication after 30 Gy dose of gamma radiation and observed the doses of 40 and 50 Gy to be completely lethal for all varieties in the study. Agreeing to our observations, stimulatory effects of lesser doses of gamma radiation on shoot multiplication have also been reported earlier [39,40].…”

Section: Number Of Shoots Bottle -1supporting

confidence: 92%

Implications of In Vitro Mutagenesis in Banana (Musa spp.)

Ali

Nizamani

Siddiqui

et al. 2020

PAB

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Banana is an important crop of Pakistan, grown on large scale in Sindh province of the country. This study was conducted to introduce genetic variability in banana variety basrai and to optimize the micropropagation protocol for the same genotype. Murashige and Skoog (MS) media supplemented with various concentrations of indole-3-acetic acid (IAA), N6benzyl aminopurine (BAP), indole-3-butyric-acid (IBA) and naphthalene acetic acid (NAA) were used. The genotypes were also radiated via. three different doses of gamma radiation i.e. 10, 20 and 30 Gy. Excellent shoot growth and elongation parameters viz. number of shoots developed bottle -1 (25.77) and shoot length (17.44 cm) were recorded in plantlets cultured on MS media supplemented with 4.00 mg l -1 IAA + 6.00 mg l -1 IBA + 4.00 mg l -1 NAA and 40 g l -1 sucrose; and radiated using 20 Gy dose of gamma radiation. The same combination of culture media and gamma rays' treatment also showed least days to shoot initiation. Minimum observations for the growth parameters were observed in plantlets radiated with 30 Gy of gamma radiation and grown in MS media containing 2.00 mg l -1 IAA + 2.00 mg l -1 BAP + 2.00 mg l -1 NAA and 40 g l -1 sucrose. Regarding treatments of gamma radiation used in the study, lower doses like 10 and 20 Gy showed stimulatory effects on plantlets' growth and development while 30 Gy was lethal. This study is significant for banana breeding as apposite induction of genetic variation through mutagenesis and optimal maintenance in the tissue culture will help in developing elite genotypes having near-commercial attributes.

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“…Irradiation of suckers prior to tissue culture initiation is not effective and can only yield low number of plantlets in the M1V1 generation because of the large sucker size, and their number is often critical for managing either physical or chemical mutagenesis. Karmarkar et al (2001) compared radiosensitivity of different in vivo and in vitro planting materials and observed a decrease in per cent survival of irradiated material with increasing irradiation dose. In terms of radiosensitivity, hardened plants were most sensitive followed by suckers and in vitro shoots.…”

Section: Mutagenesis and Induction Of Novel Genetic Variationmentioning

confidence: 99%

Genetic Diversity in Horticultural Plants

2019

Sustainable Development and Biodiversity

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Banana and plantains are one of the important fruit crops grown extensively in the tropical and subtropical regions of the world. The world production of banana is 145 million tons of which only a few million tons is exported, which means that most production is primarily for local consumption. The banana cultivars are derived from two diploid wild species, Musa acuminata (AA genome) and Musa balbisiana (BB genome). Majority of the edible banana cultivars are propagated vegetatively, and hence, the improvement of banana through conventional breeding methods is difficult. Attempts have been made to improve banana by inducing genetic variability by using both physical and chemical mutagens and exploiting the somaclonal variation a few varieties have been released for cultivation. Transgenic approach has also been used to incorporate the desirable traits into banana. Recent advances in genomics and the availability of genome sequence of both Musa acuminata and Musa balbisiana helps in the improvement of this fruit crop. Also the recent reports of genome editing through CRISPR-CAS9 will aid in speeding up the banana improvement programmes in the near future. This review summarizes the various advances made in inducing genetic diversity in banana.

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Radio-sensitivity ofin vivoandin vitrocultures of banana cv. Basrai (AAA)

Abstract: Radio-sensitivity of in vivo and in vitro cultures of banana cv. Basrai (AAA).

Cited by 15 publications

References 5 publications

Effect of gamma irradiation on the morphological and physiological variation from In vitro individual shoot of banana cv. Tanduk (Musa spp.)

Effect of gamma irradiation on the morphological and physiological variation from In vitro individual shoot of banana cv. Tanduk (Musa spp.)

Implications of In Vitro Mutagenesis in Banana (Musa spp.)

Genetic Diversity in Horticultural Plants

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