Radio-Telemetry in Biomedical Research - Radio-Telemetry Blood Pressure Measurements in Animal Models of Hypertension, How It Revolutionized Hypertension Research

Dumas, Pierre; Chiche, D.; Tremblay, Johanne; Šeda, Ondřej; Peng, Junzheng; Hamet, Pavel

doi:10.5772/24222

Cited by 1 publication

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References 107 publications

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“…BP was measured by radiotelemetry, as described in details previously. 12 The animals were euthanized by decapitation under anesthesia. After opening the abdomen, fat and connective tissues were removed, and the kidneys were isolated, weighed, rinsed in saline, and either snap-frozen in liquid nitrogen (for gene expression analysis) or stored on ice (for cell-based experiments).…”

Section: Rat Strainsmentioning

confidence: 99%

Increased Renal Epithelial Na Channel Expression and Activity Correlate With Elevation of Blood Pressure in Spontaneously Hypertensive Rats

et al. 2013

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N umerous quantitative trait loci for blood pressure (BP) elevation have been identified in essential hypertension in the last decade, followed by genome-wide association studies. [1][2][3][4][5] The high heritability of gene expression variation indicates that it may serve as an intermediate phenotype for the identification of genomic determinants of complex traits, such as essential hypertension. We initiated this approach by exploring the pleiotropic regulation of heat stress genes mapping the mRNA accumulation after heat stress and using recombinant inbred strains (RIS) developed by inbreeding the F 2 generation of normotensive Brown-Norway (BN-Lx) and spontaneously hypertensive rats (SHR). 6 We discovered a common regulator of heat stress genes, the rat heat stress transcription factor. 7 Subsequently, Hubner et al 8 undertook integrated gene expression profiling in the adipose tissue and kidneys of 6-week-old male animals from the same RIS. Dmitrieva et al 9 adopted this strategy for comparative analysis of common differentially-expressed genes in the kidneys of 4-, 8-, 12-, and 18-week-old males of 3 distinct SHR lines versus normotensive Wistar-Kyoto (WKY) rats. The renal transcriptomes of 2-week-old SHR and WKY females 10 and 9-week-old rats of both sexes 11 were compared by other research teams. However, results generated by these studies cannot serve to identify genes involved in the age-dependent development of hypertension. In the present investigation, we compared whole genome expression profiles in kidneys isolated from 12-, 40-, and 80-week-old male and female SHR and 4 RIS (HXB3, 13, 17, and 23) with different temporal patterns of hypertension development. Among the genes significantly correlating with increasing mean systolic and diastolic blood pressures, we noted the augmented mRNAs levels of Scnn1b and Scnn1g genes encoding β-and γ-epithelial Na channel (ENaC) subunits. The present study is thus focusing on the expression and activity of these genes in relation to age-dependent increase of blood pressure.Abstract-Elevation of blood pressure with age is one of the hallmarks of hypertension in both males and females. This study examined transcriptomic profiles in the kidney of 12-, 40-, and 80-week-old spontaneously hypertensive rats and 4 recombinant inbred strains in search for functional genetic elements supporting temporal dynamics of blood pressure elevation. We found that both in males and females of spontaneously hypertensive rats and hypertensive recombinant inbred strains age-dependent blood pressure increment was accompanied by 50% heightened expression of epithelial sodium channel β-and γ-subunits. Epithelial sodium channel subunit expression correlated positively with blood pressure but correlated negatively with renin expression. Increased epithelial sodium channel activity was observed in cultured epithelial cells isolated from the kidney medulla of 80-week-old spontaneously hypertensive rats but not in agematched normotensive Wistar Kyoto. This difference remained evident after ...

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Section: Rat Strainsmentioning

confidence: 99%