Radioactive environment adapted bacterial communities constituting the biofilms of hydrothermal spring caves (Budapest, Hungary)

Enyedi, Nóra Tünde; Anda, Dóra; Borsodi, Andrea K.; Pál, Sára Eszter; Óvári, Mihály; Màrialigeti, Károly; Kovács-Bodor, Petra; Mádl-Szőnyi, Judit; Makk, Judit

doi:10.1016/j.jenvrad.2019.02.010

Cited by 22 publications

(18 citation statements)

References 59 publications

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“…Concentrations of NH 4 + -N, NO 3 – N, and NO 2 – N were 0.2 mg L −1 , < 1 mg L −1 , and 0.2 mg L −1 , respectively. These results were also related to the values previously reported by Borsodi et al 6 and Enyedi et al 17 .…”

Section: Resultssupporting

confidence: 92%

“…2 ), and the low oxygen level in the spring cave may have favored their reproduction. The OTUs assigned to the phylum Chloroflexi were also frequent not only in the biofilm formed on the glass slides but in the biofilm developed on the rock surface of the RT spring cave as well 17 .…”

Section: Discussionmentioning

confidence: 93%

“…In an earlier study, a natural biofilm sample (RTB) developed for years on the rock of the RT spring cave 17 was analyzed. The taxonomic composition of bacteria inhabiting the natural and experimental biofilms was similar.…”

Section: Discussionmentioning

confidence: 99%

“…The most abundant community members of the RTB as well as the 3–30 weeks and one-year in situ model biofilm samples were also the same, showing no difference or selection between the glass slide and the carbonate rock as a surface substrate. Based on our previous results 17 , unforeseen taxonomic bacterial diversity was obtained from these highly radioactive environments (600 ± 21 Bq/L radon concentration in the case of Rudas-Török spring cave) based on next-generation sequencing data, containing mainly unclassified bacteria affiliated with low level similarity to cultured bacterial taxa. The surprisingly high diversity suggests that the microorganisms living here are well adapted to this extreme environment.…”

Section: Discussionmentioning

confidence: 99%

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In situ modelling of biofilm formation in a hydrothermal spring cave

Anda

Kovács-Bodor

Makk

et al. 2020

Sci Rep

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Attachment of microorganisms to natural or artificial surfaces and the development of biofilms are complex processes which can be influenced by several factors. Nevertheless, our knowledge on biofilm formation in karstic environment is quite incomplete. The present study aimed to examine biofilm development for a year under controlled conditions in quasi-stagnant water of a hydrothermal spring cave located in the Buda Thermal Karst System (Hungary). Using a model system, we investigated how the structure of the biofilm is formed from the water and also how the growth rate of biofilm development takes place in this environment. Besides scanning electron microscopy, next-generation DNA sequencing was used to reveal the characteristic taxa and major shifts in the composition of the bacterial communities. Dynamic temporal changes were observed in the structure of bacterial communities. Bacterial richness and diversity increased during the biofilm formation, and 9–12 weeks were needed for the maturation. Increasing EPS production was also observed from the 9–12 weeks. The biofilm was different from the water that filled the cave pool, in terms of the taxonomic composition and metabolic potential of microorganisms. In these karstic environments, the formation of mature biofilm appears to take place relatively quickly, in a few months.

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Section: Resultssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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In situ modelling of biofilm formation in a hydrothermal spring cave

Anda

Kovács-Bodor

Makk

et al. 2020

Sci Rep

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“…Since microbiological analyses of other springs of the Gellért Hill by Borsodi et al (2012Borsodi et al ( , 2018, Anda et al (2014), Makk et al (2016) and Enyedi et al (2019) proved the presence of various bacterial communities, the role of preserved microbial biofilms in concentrating a range of trace elements in the Rudas travertine should also be taken into consideration. The elevated natural radioactivity also is apparently connected to biofilms, since biogeochemical precipitates readily adsorb 226 Ra and produce 222 Rn, which although short lived might be a problem when air quality is considered (Erőss et al 2012;Kovács-Bodor et al 2019).…”

Section: Discussion Of the Observed Mineralogical Geochemical And Tementioning

confidence: 99%

INVESTIGATION OF A FLOWSTONE-LIKE HISTORICAL INDOOR-TRAVERTINE (RUDAS SPA, BUDAPEST, HUNGARY) USING THE¹⁴C “BOMB-PEAK”

et al. 2020

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Travertine precipitated during the past ca. 120 years, from thermal waters in one of the historical Spas of the Buda Thermal Karst (Hungary) was investigated using radiocarbon (14C). The age of the deposit is based on the historic date of the structure on which the travertine was deposited. A textural study of the travertine buildup using a ~22-cm-long diamond-core crosscutting was undertaken. The original aim of the study was to improve our understanding of the controls and possibly also the rate of travertine-precipitation. In addition to characteristic, mm-scale, regular laminations, 0.5–1.0 cm dark-colored intervals were also observed in the core. Correlation of these latter textural changes with well-known changes in the water management of the Spa was greatly hindered by the lack of age data from the interior of the core. Therefore, in addition to the two known points (beginning in 1883 AD and ending 2004 AD) at least one age-datum point, somewhere inbetween, was necessary. Since the timespan of the core obviously included the 1960s of the last century, we expected that the 14C anomaly related to the atmospheric nuclear tests of those years could be detected by isotope-geochemistry. This paper gives a brief overview of the textural features of the investigated travertine and presents the dataset proving the incorporation of considerable amounts of atmospheric carbon in the carbonate precipitate, which, indeed, facilitated the indirect dating of the part of the core containing “bomb” 14C, and this helped us to unfold the factors controlling the observed textural changes of the travertine.

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Response of Microbial Communities to Naturally Occurring Radioactive Material–Contaminated Sediments: A Microcosm‐Based Study

MacIntosh,

Dafforn,

Chariton

et al. 2024

Enviro Toxic and Chemistry

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There is a growing need to understand the potential ecological impacts of contaminants in offshore oil and gas infrastructure, especially if that infrastructure is to be left in situ as a decommissioning option. Naturally occurring radioactive material (NORM) is one type of contaminant found in solid deposits on internal surfaces of infrastructure that poses potential ecological harm if released into the marine environment. Microbes are important components of marine sediment ecosystems because they provide ecosystem services, yet the impacts of NORM contamination to these communities are not well understood. The present study aimed to investigate the response of benthic microbial communities to NORM‐contaminated scale, collected from an offshore oil and gas system, via controlled laboratory microcosm studies. Changes to microbial communities in natural sediment and sediments spiked with NORM at radium‐226 activity concentrations ranging from 9.5 to 59.8 Bq/kg (in partial equilibria with progeny) over 7 and 28 days were investigated using high‐throughput sequencing of environmental DNA extracted from experimental sediments. There were no significant differences in microbial community composition between control and scale‐spiked sediments over 7 and 28 days. However, we observed a greater presence of Firmicutes in the scale‐mixed treatment and Chloroflexi in the scale‐surface treatments after 28 days. This could suggest selection for species with contaminant tolerance or potential resilience to radiation and metal toxicity. Further research is needed to explore microbial tolerance mechanisms and their potential as indicators of effects of radionuclide‐contaminated sediments. The present study demonstrated that microcosm studies can provide valuable insights about the potential impacts of contamination from oil and gas infrastructure to sediment microbial communities. Environ Toxicol Chem 2024;00:1–14. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

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Radioactive environment adapted bacterial communities constituting the biofilms of hydrothermal spring caves (Budapest, Hungary)

Cited by 22 publications

References 59 publications

In situ modelling of biofilm formation in a hydrothermal spring cave

In situ modelling of biofilm formation in a hydrothermal spring cave

INVESTIGATION OF A FLOWSTONE-LIKE HISTORICAL INDOOR-TRAVERTINE (RUDAS SPA, BUDAPEST, HUNGARY) USING THE¹⁴C “BOMB-PEAK”

Response of Microbial Communities to Naturally Occurring Radioactive Material–Contaminated Sediments: A Microcosm‐Based Study

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Radioactive environment adapted bacterial communities constituting the biofilms of hydrothermal spring caves (Budapest, Hungary)

Cited by 22 publications

References 59 publications

In situ modelling of biofilm formation in a hydrothermal spring cave

In situ modelling of biofilm formation in a hydrothermal spring cave

INVESTIGATION OF A FLOWSTONE-LIKE HISTORICAL INDOOR-TRAVERTINE (RUDAS SPA, BUDAPEST, HUNGARY) USING THE14C “BOMB-PEAK”

Response of Microbial Communities to Naturally Occurring Radioactive Material–Contaminated Sediments: A Microcosm‐Based Study

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About

INVESTIGATION OF A FLOWSTONE-LIKE HISTORICAL INDOOR-TRAVERTINE (RUDAS SPA, BUDAPEST, HUNGARY) USING THE¹⁴C “BOMB-PEAK”