Radiochemical investigation of the respiration of spores of Bacillus cereus

Desser, H.

doi:10.1007/bf00412066

Cited by 9 publications

(10 citation statements)

References 8 publications

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“…While long assumed, unactivated dormant spores were shown to have near-undetectable levels of endogenous metabolism (Crook 1952;Desser and Broda 1969;Keynan and Evenchik 1969), heat activation induced temporary low levels of activity, e.g. oxidation of glucose.…”

Section: Dormancy and Longevitymentioning

confidence: 99%

History of science – spores.

Gould

2006

J Appl Microbiol

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Bacterial endospores were first studied 130 years ago by Cohn in 1876 and independently by Koch in the same year. Although spore dormancy and resistance have been much studied since then, questions still remain concerning the basic mechanisms and the kinetics of heat inactivation in particular. Likewise, the extreme dormancy and longevity of spores was recognized early on and later greatly extended but still evade complete understanding. Evidence has accumulated for the involvement of specific spore components such as calcium, dipicolinic acid, small acid soluble proteins in the core and peptidoglycan in the cortex. Involvement of physical factors too, such as the relative dehydration of the core, maybe in a high‐viscosity state or even in a glassy state, has added to appreciation of the multicomponent nature of dormancy and resistance. Spore‐former morphology formed the basis for early classification systems of sporeformers from about 1880 and consolidated in the mid‐1900s, well prior to the use of modern genetic procedures. With respect to sporulation, groundbreaking sequence studies in the 1950s provided the basis for later elucidation of the genetic control widely relevant to many cell differentiation mechanisms. With respect to the breaking of dormancy (activation and germination), the elucidation of mechanisms began in the 1940s following the observations of Hills at Porton who identified specific amino acid and riboside ‘germinants’, and laid the basis for the later genetic analyses, the identification of germinant receptor genes and the elucidation of key germination reactions. The nonexponential nature of germination kinetics has thwarted the development of practical Tyndallization‐like processing. So inactivation by heat remains the premier method of spore control, the basis of a huge worldwide industry, and still relying on the basic kinetics of inactivation of Clostridium botulinum spores, and the reasoning regarding safety first evolved by Bigelow et al. in 1920 and Esty and Meyer in 1922. ‘Newer’ processes such as treatment with ionizing radiation (first proposed in 1905) and high hydrostatic pressure (first proposed in 1899) may be introduced if consumer resistance and some remaining technical barriers could be overcome.

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Section: Dormancy and Longevitymentioning

confidence: 99%

History of science – spores.

Gould

2006

J Appl Microbiol

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“…In microbial spores, respiration rates are reduced by factors of 20 to 100, relative to that in vegetative cells (Sussman 1966, Dresser and Broda 1969, Sussman 1969. Note that these reductions, which are comparable to.…”

Section: The Conditions Necessary For Germination In Dinoflagellate Cmentioning

confidence: 99%

“…3), are for hydrated spores. Dessication of these same spores results in far greater respiratory reductions without a loss in viability (Dresser and Broda 1969). Likewise, respiratory activity in "dry" plant seeds is greatly reduced compared to that of imbibed (but still dormant) seeds (Bewley andBlack 1979, 1982).…”

Section: The Conditions Necessary For Germination In Dinoflagellate Cmentioning

confidence: 99%

The physiology of dormancy and germination in cysts of the marine dinoflagellate Scrippsiella trochoidea

Binder¹

1986

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“…Bacterial endospores are amongst the most dormant living organisms known and also amongst the most resistant. Their extreme dormancy is illustrated by the fact that their endogenous metabolism may be undetectable, even using the mosc sensitive possible radio tracer techniques (Desser & Broda 1969). Their extreme resistance is illustrated by the fact that exposure to a temperature as high as 120 "C for more than 10 min is necessary to kill about 90% of the spores of some organisms when suspended in aqueous media (Roberts & Hitchins 1969), and exposure at about 165 O C for more than 10 min is needed to similarly inactivate spores of Bacillus spp.…”

Section: Characteristics Of Bacterial Endosporesmentioning

confidence: 99%

Recent Advances in the Understanding of Resistance and Dormancy in Bacterial Spores

Gould

1977

Journal of Applied Bacteriology

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Radiochemical investigation of the respiration of spores of Bacillus cereus

Cited by 9 publications

References 8 publications

History of science – spores.

History of science – spores.

The physiology of dormancy and germination in cysts of the marine dinoflagellate Scrippsiella trochoidea

Recent Advances in the Understanding of Resistance and Dormancy in Bacterial Spores

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