Radiochemical quantitation of conjugated dienes during ischemia and reperfusion in the rat liver

Hashimoto, Noriaki; Wu, Tai-Wing; Au, Jian-Xun; Sugiyama, Hiroshi; Mickle, Donald A.G.; Weisel, Richard D.; Carey, Douglas

doi:10.1016/0009-9120(91)90481-s

Cited by 8 publications

(3 citation statements)

References 21 publications

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“…I4C-TCNE undergoes a specific Diels-Alder condensation with conjugated dienes (CD); the radioactivity in the adduct is used to quantitate CD. The TCNE assay is more selective than other methods for CD or for lipoperoxides and is done at room temperature (17)(18). Note that Trolox between 0.5 and 16 mmoVL did not affect the quantitation of 2,4 hexadiene standard (0 to 120 nmol/L) in this method.…”

Section: Methodsmentioning

confidence: 86%

“…Quantitation of CDs in Rat Liver Tissue. TO gain more biochemical insight into the extent of oxyradical damage in uiuo, we quantitated the CDs in the phospholipid extracts of rat tissue at different times during ischemia and reperfusion, using the 14C-TCNE method (17) modified by us (18). Figure 3 shows the profiles of CD appearance in the rat liver during global ischemia and reperfusion after treatment with either saline (control) or Trolox (3 ml of 16 mmoVL).…”

Section: Resultsmentioning

confidence: 99%

“…Quantitation of Phospholipid Coqjugated Dienes. The method of Waller and Recknagel(17) modified according to us (18) was applied to phospholipid extracts of liver tissue, using 14C-tetracyanoethylene (TCNE). I4C-TCNE undergoes a specific Diels-Alder condensation with conjugated dienes (CD); the radioactivity in the adduct is used to quantitate CD.…”

Section: Methodsmentioning

confidence: 99%

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Trolox protects rat hepatocytes against oxyradical damage and the ischemic rat liver from reperfusion injury

Hashimoto

et al. 1991

Hepatology

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Trolox, a hydrophilic analog of vitamin E, was reported to scavenge peroxyl radicals from artificial systems better than its parent compound. Here we examined the possible cytoprotective effect of Trolox in cultured hepatocytes and in the rat liver. In cultured rat hepatocytes, 0.5 to 16 mmol/L Trolox (with optimum between 1 to 2 mmol/L) was observed to prolong the survival of cells exposed to oxyradicals generated with xanthine oxidase-hypoxanthine. The protection by 1 mmol/L Trolox surpassed that provided by either ascorbate, mannitol, superoxide dismutase and/or catalase--each at a level giving its maximal protection in the same system. In both a global and partial model of hepatic ischemia-reperfusion in rats, infusion of Trolox (7.5 to 10 mumol/kg body weight) just before reflow reduced by greater than 80% the liver necrosis sustained in untreated (no Trolox) control rats. Such organ salvage was apparently accompanied by approximately 50% reduction in the amount of hepatic conjugated dienes, which were quantified by a highly specific radiochemical assay. Since conjugated dienes are presumed to be good "markers" of oxyradical damage, our data may have provided a semiquantitative link between free radical-induced necrosis and its chemical imprint in vivo. The data also indicated a relatively rapid and potent antioxidant-like action by Trolox on rat hepatocytes and on the postischemic reperfused rat liver.

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Section: Methodsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Trolox protects rat hepatocytes against oxyradical damage and the ischemic rat liver from reperfusion injury

Hashimoto

et al. 1991

Hepatology

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Enhancement in antioxidant-based hepatoprotective activity of trolox by its conjugation to lactosylphenylpyranoside

Pristupa

Zeng

et al. 1992

Hepatology

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When Trolox (a polar analog of vitamin E) is conjugated to p-aminophenyl-beta-D-lactopyranoside, the resulting lactosylphenyl Trolox becomes a markedly more stable and effective hepatoprotector than Trolox. In primary rat hepatocytes exposed to xanthine oxidase-hypoxanthine, lactosylphenyl Trolox prolonged cell survival better than did Trolox, mannitol or ascorbate. In rats that underwent 80-min partial hepatic ischemia, infusion of lactosylphenyl Trolox at 2.9 to 5.7 mumol/kg body wt just before reoxygenation salvaged the organ more extensively than did Trolox. Mechanistically, we showed (a) that lactosylphenyl Trolox does not inhibit xanthine oxidase; (b) that lactosylphenyl Trolox effectively scavenges oxyradicals generated with xanthine oxidase and the peroxyl radicals produced with 2,2'-azo-bis(2-amidinopropane) HCl; (c) that both in hepatocytes and in vivo, lactosylphenyl Trolox is distinctly more cytoprotective than either or both of its precursors; and (d) that lactosylphenyl Trolox is amphipathic (i.e., it has both hydrophilic and hydrophobic properties), which enable it to better access and protect the lipid and aqueous milieus of the cell than the lipophile vitamin E and the moderately polar Trolox. Thus there are strong fundamental reasons for lactosylphenyl Trolox being an effective antioxidant-based hepatoprotector.

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Propyl gallate as a hepatoprotector in vitro and in vivo

Wu¹,

Fung

Zeng

et al. 1994

Biochemical Pharmacology

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Radiochemical quantitation of conjugated dienes during ischemia and reperfusion in the rat liver

Cited by 8 publications

References 21 publications

Trolox protects rat hepatocytes against oxyradical damage and the ischemic rat liver from reperfusion injury

Trolox protects rat hepatocytes against oxyradical damage and the ischemic rat liver from reperfusion injury

Enhancement in antioxidant-based hepatoprotective activity of trolox by its conjugation to lactosylphenylpyranoside

Propyl gallate as a hepatoprotector in vitro and in vivo

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