Radioelectroimmunoassay of Anti-D Immunoglobulin on Agarose Plates Containing Whole Red Cells

Abstract

By incorporating whole Rh(+) red cells in an agarose plate and adding a small amount of purified 125 I anti-D immunoglobulin as a tracer to standards and samples, a radioelectroimmunoassay (REIA) of anti-D immunoglobulin was possible. The results by this method agree with those obtained by the direct method. The coefficient of variation (within plate precision) is less than 9% and the reproducibility is 12% (coefficient of variation for a plasma sample assayed on six occasions during 1 month). The International anti-D serum was compared with the WHO standard preparation 68/419 on five occasions and found to contain 11; 11; 10; 9 and 11 µg per ampoule.