Radioelectrophoresis for Determination of Thyroid Hormone Binding Abnormalities in Human Serum

Abstract

j Summary: This paper describes a rapid and accurate method for determining binding abilities of thyroid \ hormones to their corresponding serum proteins: prealbumin, albumin and thyroxine binding globulin. A tube cell agarose gel electrophoresis is used with radioactive labelled triiodothyronine or thyroxine. The distribution curve shows characteristic peaks for prealbumin, albumin and thyroxine binding globulin.A collective of 60 euthyroid thyroid-healthy persons was examined, and the following reference values for the binding of thyroxine to its binding proteins were found: The percentage binding of thyroxine to the prealbumin fraction is 21 to 33%, to the albumin fraction 10 to 15% and to the thyroxine binding globulin fraction 54 to 66%. In a group of 220 patients with suspected thyroid hormone binding abnormalities there were 42 cases of albumin-associated hyperthyröxinaemia, 5 cases of prealbumin-associated hyperthyroxinaemia and 2 cases of thyroxine binding globulin deficiency. In another group of 35 patients with suspected antibodies against thyroid hormones one patient showed antibodies against triiodothyronine.

Hormone Disruption by PBDEs in Adult Male Sport Fish Consumers

“…We examined the distribution of T 4 binding in plasma by radioelectrophoresis (Borst et al 1982; Leopold et al 1987). We separated albumin- and TBG-bound 125 I-T 4 on agarose gels after incubation of 125 I-T 4 with the serum for 2 hr at 37°C.…”