1985
DOI: 10.1128/jcm.21.3.419-424.1985
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Radioenzymatic assays for aminoglycosides with kanamycin 6'-acetyltransferase

Abstract: To facilitate the rapid and accurate quantitation of parenterally administered aminoglycosides, we defined the optimum conditions (pH, duration of incubation, and cofactor concentrations) to permit radioenzymatic assays with kanamycin acetyltransferase. The accuracy in quantitating tobramycin, netilmicin, kanamycin, and amikacin at concentrations in the therapeutic range was greater than 90%, with a mean recovery of 102.8%. The mean of the interassay coefficient of variation was 7.8%. Typical standard curves a… Show more

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Cited by 10 publications
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“…Several methods have been applied for determination of amikacin in various matrices including microbiological assays , radioenzymatic assays , and immunoassays . Chromatographic methods have been used in the analysis of AGs including TLC , GC , and LC .…”
Section: Introductionmentioning
confidence: 99%
“…Several methods have been applied for determination of amikacin in various matrices including microbiological assays , radioenzymatic assays , and immunoassays . Chromatographic methods have been used in the analysis of AGs including TLC , GC , and LC .…”
Section: Introductionmentioning
confidence: 99%