Radiofrequency ablation of the great saphenous vein, comparing one versus two treatment cycles for the proximal vein segment

Sufian, Shekeeb; Arnez, Alejandro; Labropoulos, Nicos; Nguyen, Khanh P.; Satwah, Vinay; Marquez, Jocellie; Chowla, Arun; Lakhanpal, Sanjiv

doi:10.1177/0268355514556142

Cited by 8 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The GSV was cannulated at the most distal point of venous reflux, and the catheter tip was positioned 2 cm distal to the saphenofemoral junction. The GSV was ablated with the concomitant phlebectomy [30,31]. The specimen collected from the avulsion phlebectomy were used for analysis.…”

Section: Methodsmentioning

confidence: 99%

Global Expression Profiling Identifies a Novel Hyaluronan Synthases 2 Gene in the Pathogenesis of Lower Extremity Varicose Veins

Hsieh

Tsai

Chen

et al. 2018

JCM

View full text Add to dashboard Cite

Lower extremities varicose veins (VV) are among the most easily recognized venous abnormalities. The genetic mechanism of VV is largely unknown. In this study, we sought to explore the global expressional change of VV and identify novel genes that might play a role in VV. We used next-generation ribonucleic acid (RNA) sequence (RNA seq) technology to study the global messenger RNA expressional change in the venous samples of five diseased and five control patients. We identified several differentially expressed genes, which were further confirmed by conventional reverse transcription polymerase chain reaction (RT-PCR). Using these significant genes we performed in silico pathway analyses and found distinct transcriptional networks, such as angiogenesis, cell adhesion, vascular injury, and carbohydrate metabolisms that might be involved in the mechanism of VV. Among these significant genes, we also found hyaluronan synthases 2 gene (HAS2) played a pivotal role and governed all these pathways. We further confirmed that HAS2 expression was decreased in the venous samples of patients with VV. Finally, we used a zebrafish model with fluorescence emitting vasculature and red blood cells to see the morphological changes of the venous system and blood flow. We found that HAS2 knockdown in zebrafish resulted in dilated venous structural with static venous flow. HAS2 may modulate the transcriptional networks of angiogenesis, cell adhesion, vascular injury, and carbohydrate metabolisms in venous tissues and downregulation of HAS2 may underlie the mechanism of VV.

show abstract

Section: Methodsmentioning

confidence: 99%

Global Expression Profiling Identifies a Novel Hyaluronan Synthases 2 Gene in the Pathogenesis of Lower Extremity Varicose Veins

Hsieh

Tsai

Chen

et al. 2018

JCM

View full text Add to dashboard Cite

show abstract

“…Hence, papers using this segmental type RFA usually just quote ‘cycles’. 17 However, when EVTA is performed with any thermal device and the power is known and the length of vein known, then LEED is an acceptable measure to quote. Indeed, it has been used for RFiTT RFA previously.…”

Section: Discussionmentioning

confidence: 99%

Linear endovenous energy density (LEED) should always be quoted with the power used in endovenous thermal ablation – results from an in-vitro porcine liver model study

et al. 2023

View full text Add to dashboard Cite

Background Linear Endovenous Energy Density (LEED) is the energy used per cm of vein in endovenous thermal ablation (EVTA) but does not include time. This study examines the effect of time during EVTA. Method EVTA was performed in a previously validated porcine liver model. LEEDs of 40, 60, 80 and 100 J/cm, using different powers were repeated 5 times each. Thermal spread, tissue carbonisation and device-tissue sticking during treatment were recorded. Result LEED positively correlated to thermal spread and carbonisation of the tissue. Power was correlated with carbonisation but not thermal spread. Pullback had no correlations with thermal spread or carbonisation. Catheter sticking found occurred in powers >= 15 W or LEED >= 80 J/cm. Conclusion LEED is a good measure of EVTA but does not include time. Power, which does include time, correlates with carbonisation and with device-tissue sticking. The power used must be quoted with the LEED.

show abstract

“…Hence papers using this segmental type RFA usually just quote "cycles". 18 However, when ETVA is performed with any thermal device and the power is known and the length of vein known, then LEED is an acceptable measure to quote. Indeed, it has been used for RFiTT RFA previously 11 .…”

Section: Discussionmentioning

confidence: 99%

Linear Endovenous Energy Density (LEED) Should Always be Quoted with the Power Used, in Endovenous Thermal Ablation - Results from an In-Vitro Porcine Liver Model Study.

Nielsen¹,

Croucher²,

Muschamp³

et al. 2022

Preprint

View full text Add to dashboard Cite

Introduction: Endovenous thermal ablation (EVTA) is the first line treatment of symptomatic truncal venous reflux. Linear Endovenous Energy Density (LEED) is the measure of energy used per cm of treated vein. The rate of application of energy influences thermal spread and tissue damage. However, time is absent from LEED. The aim of this study was to test the effect of time during EVTA in a validated in-vitro model.Methods: We used a bipolar radofrequency catheter and the previously validated porcine liver model to assess any thermal effects. We used combinations of power and pullback speeds giving LEEDs of 40, 60, 80 and 100 J/cm. High-resolution digital images of the ablation tracts were taken. Each setting was repeated 5 times. Thermal spread was measured using digital measuring software, tissue carbonisation assessed by a defined scale and any device-tissue sticking during treatment was recorded.Results: LEED was significantly positively correlated to thermal spread (r(20) = .86, P < .00001) and carbonisation of the tissue (r(24) = .82, p < .00001). Power was significantly correlated with carbonisation (r(24) = .50, p = .009) but not with thermal spread (r(20) = .34, p = .121). Pullback had no significant correlations with thermal spread or carbonisation. Catheter sticking was only found when the power was >= 15 W or the LEED was >= 80 J/cm.Conclusion: LEED is a good measure of EVTA but does not take into account the time of energy application to tissue. Power, which does include time, has a significant correlation with tissue carbonisation, and is also important in device-tissue sticking. Pullback has no significant correlation to any tissue effects. Quoting the LEED alone in reporting EVTA is inadequate. It is essential to include the power used when LEED is quoted.

show abstract

Radiofrequency ablation of the great saphenous vein, comparing one versus two treatment cycles for the proximal vein segment

Cited by 8 publications

References 12 publications

Global Expression Profiling Identifies a Novel Hyaluronan Synthases 2 Gene in the Pathogenesis of Lower Extremity Varicose Veins

Global Expression Profiling Identifies a Novel Hyaluronan Synthases 2 Gene in the Pathogenesis of Lower Extremity Varicose Veins

Linear endovenous energy density (LEED) should always be quoted with the power used in endovenous thermal ablation – results from an in-vitro porcine liver model study

Linear Endovenous Energy Density (LEED) Should Always be Quoted with the Power Used, in Endovenous Thermal Ablation - Results from an In-Vitro Porcine Liver Model Study.

Contact Info

Product

Resources

About