Radioimaging of human glioma xenografts with 123I labeled monoclonal antibody G-22 against glioma-associated antigen

Abstract

Monoclonal antibody (MCA) G-22 is directed against a human glioma-associated surface antigen. Its availability for the radioimmunodetection of human glioma was analyzed by utilizing the xenografts in athymic mice. Nude mice with subcutaneous grafts of U251-MG or U251-SP glioma received intravenous administration of 123I or 131I labeled F(ab')2 fragment or whole immunoglobulin. Results of radioimaging revealed that 123I-labeled antibody was better than the 131I-labeled. It was also noted that administration of 123I-labeled F(ab')2 fragment of G-22 MCA enabled the imaging of human glioma xenografts weighing 80-650 mg after 48 hours. When biodistribution of 123I MCA was compared between G-22 and control antibodies, the percentages of dose/g in tumors were 5.228-1.799 at 30 hours and 4.112-1.132 at 48 hours with G-22 and they were 4.164-1.248 and 0.314-0.142 with control. The tumor/blood ratio until 72 hours after injection was constantly above 1 with G-22 and less than 1 with control antibody. These results indicate the potential usefulness of G-22 MCA for the radioimmunodetection of human gliomas.

Recurrent malignant glioma: detection with131I labeled monoclonal antibody G-22, positron emission tomography and magnetic resonance imaging

“…8 The uptake of high-grade glioma was high, but that of low-grade gliomas was lows In the experimental study, a clear image of a human glioma subcutaneous tumor was obtained with a 123I labeled G-22 F(ab')2 fragment. 9 In this study, the radioimaging of 131I G-22 showed a marked uptake in the left temporo-parietal area. Histological examination after intracranial surgery revealed recurrent glioblastoma (Fig.…”

Role of GalNAc4S-6ST in Astrocytic Tumor Progression

“…Human glioblastoma U251-MG cells were kindly provided by Professor Jun Yoshida, Department of Neurosurgery, Nagoya University Graduate School of Medicine, Nagoya, Japan [20]. Cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Life Technologies, Carlsbad, CA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; HyClone Laboratories, Logan, UT, USA) at 37°C in 5% CO 2 .…”