Radioimmunoassay for prednisolone

Colburn, Wayne A.; Buller, R.

doi:10.1016/0039-128x(73)90124-4

Cited by 67 publications

(29 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One study reported PK parameters at a single administration of PDS (1 mg/kg) to beagles (Van der Heyden et al 2012) and another study investigated PK parameters at doses of 5 mg and 30 mg (Colburn andBuller 1973, Tse andWelling 1977). In humans, a clinical oral dosing of PDS at 25 mg to 60 mg, were studied (Lee 1991, Mollmann et al 1995.…”

Section: Resultsmentioning

confidence: 99%

Comparison of body surface area-based and weight-based dosing format for oral prednisolone administration in small and large-breed dogs

Nam¹,

Kim²,

Jeong³

et al. 2017

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

This study compared the pharmacokinetics of Prednisolone (PDS) in small-and large breed dogs with a dosing format based on body surface area (BSA) or body weight (BW). The maximum concentration and area under the curve in large-breed dogs orally administered 2 mg/kg PDS were significantly greater than those in small-breed dogs given 2 mg/kg and in large-breed dogs given 40 mg/m 2 . The higher blood concentrations that result from BW-based dosing of oral PDS in large-breed dogs can be more than required for effect. Meanwhile, BSA dosing at 40 mg/m may be suboptimal. These findings confirm important differences between standard PDS dosing schemes in dogs while highlighting the need to further optimize PDS dosing in large-breed dogs.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparison of body surface area-based and weight-based dosing format for oral prednisolone administration in small and large-breed dogs

Nam¹,

Kim²,

Jeong³

et al. 2017

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…This problem has been overcome by the use of phase-separating paper. Radioimmunoassay techniques may be applied to the estimation of plasma prednisolone (Colburn & Buller, 1973;Sullivan, Stoll, Sakmar, Blair & Wagner, 1974). Cortisol however cross-reacts with the primary antibody and therefore interferes in this technique.…”

Section: Prednisolone Half-lives and Profilesmentioning

confidence: 99%

“…Recently, several groups have developed sensitive methods for the estimation of plasma prednisolone based on competitive protein binding or radioimmunoassay (English et aL, 1974;Turner, Carroll, Pinkus, Charles & Chattoraj, 1973;Colburn & Buller, 1973).…”

Section: Introductionmentioning

confidence: 99%

Measurement of plasma prednisolone in man.

Wilson¹,

Ssendagire²,

May³

et al. 1975

Brit J Clinical Pharma

View full text Add to dashboard Cite

1. A modification of the competitive protein binding assay for prednisolone described by English, Chakraborty & Marks (1974) is presented. 2. Addition of pg quantities of [3H]‐prednisolone following oral dosage with prednisone or prednisolone. 3. Mean plasma half‐life and peak plasma concentrations in patients with respiratory disease seen following prednisolone administration agree well with previous studies in healthy volunteers performed by other workers using competitive protein binding and radioimmunoassay techniques. 4. A considerable inter‐patient variation in bioavailability after a standard dose of prednisolone (20 mg) was noted.

show abstract

“…With the development of radioimmunoassays for prednisone (1) and its metabolite, prednisolone (2), it is now possible to measure the plasma levels of these steroids following the oral administration of a single, low dose of prednisone. The early observations, of Campagna et al (3) and Levy et al (4) demonstrated a potential bioavailability problem with prednisone tablets which only recently could begin to be assessed.…”

Section: Introductionmentioning

confidence: 99%

Comparative bioavailability: Eight commercial prednisone tablets

Sullivan

Hallmark

Sakmar

et al. 1976

Journal of Pharmacokinetics and Biopharmaceutics

View full text Add to dashboard Cite

Two four-treatment crossover bioavailability studies were performed in panels of 12 adult male volunteers with eight different commercial prednisone tablets. Plasma samples from the first study were assayed by radioimmunoassay for both prednisone and prednisolone. Plasma samples from the second study were assayed for prednisolone only. Statistical analyses of the data showed significant differences in the rate of appearance of prednisolone in plasma, but not in the amount converted to prednisolone. Some observations are made on the relationships between prednisone and prednisolone concentrations in plasma following oral ~lministration of prednisone.KEY WORDS: prednisone bioavailability; elimination half-lives of prednisone and prednisolone; prednisone and prednisolone plasma levels following prednisone. INTRODUCTIONWith the development of radioimmunoassays for prednisone (1) and its metabolite, prednisolone (2), it is now possible to measure the plasma levels of these steroids following the oral administration of a single, low dose of prednisone. The early observations, of Campagna et al. Sullivan, Hallmark, Sakmar; Weidler, Earhart, and Wagner reported data on some prednisone tablets. One of these (6) reported results of our first prednisone study.It is well documented that prednisone is metabolized to prednisolone (7-9), which is assumed to be its active form. However, in most of these studies the metabolites of prednisolone have been isolated only from urine, or plasma levels of prednisolone have been measured following high doses of prednisone. In only one unpublished study of DiSanto et al. has the determination of both prednisone and prednisolone in plasma been undertaken.This article reports the results of two four-treatment crossover studies with commercially available prednisone tablets. In the second prednisone study both prednisone and prednisolone plasma levels were measured, while in the third prednisone study only prednisolone was measured in plasma. All tablets utilized in the studies were purchased on the open market by the Food and Drug Administration. EXPERIMENTAL In Vitro StudiesDissolution testing was performed in the laboratories of the Food and Drug Administration according to the specifications of the USP XVIII. Results of dissolution rate tests on the same tablets by another method will be reported in a subsequent publication. SubjectsTwelve adult male volunteers between the ages of 21 and 32 years, weighing between 60.3 and 82.6 kg and in good health, were selected for each study. Six of the subjects participated in both studies. Each subject received a medical history, physical examination, and blood and urine analysis including plasma 17-OHCS.All subjects selected received no barbiturates or other enzyme-inducing agents for a period of 30 days preceding the study until its completion. They received no other medication or alcoholic beverages for a period beginning 7 days preceding the study until completion of the study. The subjects were assigned numbers which were ordered according t...

show abstract

Radioimmunoassay for prednisolone

Cited by 67 publications

References 14 publications

Comparison of body surface area-based and weight-based dosing format for oral prednisolone administration in small and large-breed dogs

Comparison of body surface area-based and weight-based dosing format for oral prednisolone administration in small and large-breed dogs

Measurement of plasma prednisolone in man.

Comparative bioavailability: Eight commercial prednisone tablets

Contact Info

Product

Resources

About