Radioimmunoassay for quantifying antibody to N. Gonorrhoeae in human sera.

Luoma, Marianna; Cross, William; Rudbach, Jon A.

doi:10.1136/sti.51.6.387

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…This represents a population with a prevalence of 100%. Similar results for an RIA were reported by Luoma et al (20), again in a preselected population of culture-positive and -negative sera.…”

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confidence: 88%

Radioimmunoassay for detection of antibodies to Neisseria gonorrhoeae

Usategui¹,

Savard²,

Mondabaugh³

et al. 1982

J Clin Microbiol

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A radioimmunoassay has been developed and evaluated for the serological diagnosis of gonorrhea. Purified gonococcal antigen was obtained from a culture of Neisseria gonorrhoeae (B370) and labeled with 1251 for use in a double-antibody test system. The test was evaluated in populations segregated by sex and risk. The specificity of the assay in females was 90.2% (55/61) in low risk, 82.2% (2,245/ 2,732) in medium risk, and 54.1% (335/619) in high risk. The sensitivity was 69% (20/29) in medium risk and 78.3% (288/367) in high risk. In males, test specificity was 92.3% (24/26) in low risk and 50% (48/96) in high risk. The sensitivity was 70.8% (143/202) in the high-risk group. The data in this study indicate that this assay should not be employed for screening of either highor medium-risk populations.

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confidence: 88%

Radioimmunoassay for detection of antibodies to Neisseria gonorrhoeae

Usategui¹,

Savard²,

Mondabaugh³

et al. 1982

J Clin Microbiol

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“…A variety of serological techniques have been described, including the complement fixation test (4,26), flocculation (18, 31), direct hemagglutination (9,19,32), and the indirect immunofluorescence test (2,34,35). Recently, a radioimmunoassay (1,21) and an indirect hemagglutination test (25) in which gonococcal pilus antigen is used to detect gonococcal antibodies have been described, but the results of these tests have been variable because of a lack of sufficient sensitivity or specificity. Except for the tests based on purified pili, the existing serological tests use either whole gonococci or chemically undefined extracts as antigens.…”

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confidence: 99%

Passive hemagglutination test for detection of antibody to gonococcal ribosomal antigen in sera from patients with asymptomatic gonorrhea

Kita¹,

Kashiba²

1982

J Clin Microbiol

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Ribosomal fractions were obtained from a culture of type 2 Neisseria gonorrhoeae strain P-17 which was isolated from a patient with an acute gonococcal infection; these fractions were purified to eliminate the components of the outer membrane complex by affinity chromatography (Sepharose-anti-outer membrane complex antibody conjugates were used as the solid immunosorbent), and the resulting preparation was designated the purified ribosomal fraction. The purified ribosomal fraction was used to detect antibody activity in sera obtained from culture-positive asymptomatic carriers and healthy controls by a passive hemagglutination test. This passive hemagglutination test had a specificity of 100% for both sexes and sensitivities of 99.4 and 88.2% for female and male carriers, respectively, when an antibody titer of more than 1:3 was defined as abnormal. Absorption of the sera with nongonococcal organisms did not affect the antibody activity, and no significant difference in antigenicity among various N. gonorrhoeae strains was observed in ribosomal fractions. An enzyme-linked immunosorbent assay was also used to measure the relative amounts of specific antibodies to the purified ribosomal fraction, and this assay revealed that the anti-purified ribosomal fraction antibodies were immunoglobulin G.

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“…M A N Y recent attempts to provide a serological diagnosis of gonorrhoea aim to detect antibodies by sensitive methods such as radioimmunoassay (RIA) (Buchanan et al, 1973;Luoma, Cross and Rudbach, 1975;Oates et al, 1977), or immunofluorescence (O'Reilly, Welch and Kellogg, 1973 ;Welch and O'Reilly, 1973;Wilkinson, 1975;Gaafar, 1976;Gaafar and d'Arcangelis, 1976). In general, diagnostic methods based on detection of antibodies have two disadvantages : delay before detectable levels of antibody are produced, and the persistence of antibodies from a past infection which may lead to disease being diagnosed when it is absent.…”

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Detection of Gonococcal Antigens in Urine by Radioimmunoassay

Thornley

Wilson

Hormaeche

et al. 1979

Journal of Medical Microbiology

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a test for antigen in sediment had to be devised. In this, two main problems were encountered : non-specific binding of radioactively labelled IgG by substances present in urine, especially in the sediments, and inhibition of the assay by substances present in urine. Supernatants and sediments were clearly inhibitory, but the inhibition could not be studied quantitatively in the presence of non-specific binding. A study, described elsewhere (Thornley et al., 1979), was therefore made of the inhibition caused by supernatants; methods of preventing it were devised and applied in the experiments described here to neutralise inhibition when urine sediments were being tested for gonococcal antigen.Thereafter, ways of reducing the non-specific binding of antibody by urine sediments were examined, and because some non-specific binding could not be removed, a test distinguishing between non-specific binding of antibody and its specific binding to gonococcal antigen was devised. This paper describes: the RIA of gonococcal antigens in a buffer system; the specificity test devised to detect gonococcal antigens in urine sediments by RIA, and the prevention of inhibitory action; and the results obtained when this method was used to test the sediments of urine samples from male patients with gonorrhoea or non-specific urethritis, and urine sediments from a small number of female patients. MATERIALS AND METHODSGonococci were isolated from urethral, vaginal or cervical swabs, or urine samples, from the Genito-Medical Clinic, Addenbrooke's Hospital or from the Public Health Laboratory Cambridge, by culture on chocolate (heated blood) -agar plates incubated for 24 or 48 h at 37°C in candle jars. Gonococci were identified by colony morphology, morphology after Gram staining, oxidase reaction, and fermentation tests. Rough counts of the number of gonococci in urine were made by spreading 0.1 ml of the sample over one third of a chocolateagar plate, and streaking out for single colonies over the remainder. After incubation, the number of small translucent colonies composed of oxidase-positive gram-negative diplococci was recorded as: 500.

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Radioimmunoassay for quantifying antibody to N. Gonorrhoeae in human sera.

Cited by 3 publications

References 12 publications

Radioimmunoassay for detection of antibodies to Neisseria gonorrhoeae

Radioimmunoassay for detection of antibodies to Neisseria gonorrhoeae

Passive hemagglutination test for detection of antibody to gonococcal ribosomal antigen in sera from patients with asymptomatic gonorrhea

Detection of Gonococcal Antigens in Urine by Radioimmunoassay

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