Radioimmunoassay of Dibenzazepines and Dibenzcycloheptanodienes in Body Fluids and Tissues

Abstract

Sumraary: In an attempt to establish a radioimmunoassay (RIA), imipramine and amitriptyline immunogens were prepared; desmethyl derivatives were converted into hemisuccinates, conjugated with bovine serum albumin and used for rabbit immunization. [ 3 H]Amitriptyline (4.3 TBq/mmol) and [ 3 H]imipramine (2.9 TBq/mmol) were prepared by catalytic dehalogenation or reductive alkylation. Dibenzazepines and dibenzcycloheptanodienes were determined in biological fluids by a direct method without deproteinization (lower detection limit of 0.5 μg • l" 1); using high-yield methods they were extracted from cell membranes. Assay of tricyclic antidepressants in humans showed that these substances disappear from plasma much earlier than from cell membranes. Dissociation of the antidepressants bound to cell membranes *is slow and their plasma concentrations are not influenced by Standing for 2 h at 4 °C. During preparing the membranes for binding studies these substances are not removed, and they may affect the results of the binding studies.

A validated spectrofluorimetric method for determination of some psychoactive drugs

“…These methods include; spectrophotometry [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18], spectrofluorimetry [19][20][21][22][23][24], titrimetry [25][26][27][28][29][30], chromatography [31][32][33][34][35][36][37][38][39][40][41][42], flow injection analysis [43][44][45][46][47], radio-immunoassay [48][49], electrochemical methods [50][51] and chemiluminescence methods [52]…”

The Use of Quercetin for Spectrophotometric Determination of Some CNS Acting Drugs

“…These methods include; spectrophotometry, spectrofluorimetry, [23][24][25][26][27][28][29] titrimetry, [30][31][32][33][34][35] chromatography, [36][37][38][39][40][41][42][43][44][45][46][47][48] flow injection analyses, [49][50][51][52][53] radio-immunoassay, [54][55][56] electrochemical methods [57][58] and chemiluminescence methods. [59][60][61] In the present work oxidized quercetin is used for extractive spectrophotometric determination of seven CNS acting drugs which are listed in Table 1.…”

Sensitive Spectrophotometric Determination of Some Dibenzazepine Drugs with Diazotized P-Phenylenediamine Dihydrochloride

“…The vast commercial success of these medicinal agents has made the chemistry of dibenzazepines to become a major area of research in heterocyclic chemistry and an important branch of commercial importance in pharmaceutical sciences 1 ' 3 . Many contemporary analytical methodologies, including titrimetry, 7 UV-visible spectrophotometry 8 ' 16 , voltammetry 17 ' 21 , ion selective electrodes 22 , potentiometry 23 ' 24 , amperometry 2425 , radioimmunoassay 26 , and chromatographic methods 27 " 28 have been utilised to determine these drugs in different matrices. However, these methods have proved to be deficient with respect to specificity, sensitivity, simplicity, and or short analysis time.…”