Radioimmunoassay of motilin

Lee, Kae Yol; Chey, William Y.; Tai, Hsin‐Hsiung; Yajima, Haruaki; Wagner, David R.

doi:10.1007/bf01079787

Cited by 122 publications

(3 citation statements)

References 25 publications

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“…Preparation of Enzyme-Labeled Antigen Human somatostatin, porcine motilin, mini gastrin I, and fragment VIP (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28) were conjugated with b-D-galactosidase (Boehringer Mannheim, Mannheim, Germany) with N-(e-maleimidocaproyloxy) (EMC)-succinimide according to the methods of Kitagawa et al 22) These peptides dissolved in 0.05 M phosphate buffer, pH 7.0, were mixed with EMC-succinimide in tetrahydrofuran at 20°C for 40 min. The EMCpeptides thus obtained were purified via separation on a Sephadex G-25 column.…”

Section: Methodsmentioning

confidence: 99%

“…[19][20][21] However, in terms of safety, sensitivity, and ease of handling, RIA methods are still less than satisfactory. We developed a sensitive and specific double-antibody enzyme immunoassay (EIA) for detecting motilin, using motilin-linked b-D-galactosidase as a marker antigen, a secondary antibody-coated immunoplate, and 4-methylumbelliferyl-b-D-galactopyranoside as a fluorogenic substrate.…”

Section: )mentioning

confidence: 99%

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Hange-shashin-to Raises Levels of Somatostatin, Motilin, and Gastrin in the Plasma of Healthy Subjects.

Naito

Itoh

Yasunaga

et al. 2002

Biological & Pharmaceutical Bulletin

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Somatostatin is a gastrointestinal motility regulator and functions as an inhibitor of hormone release in digestive organs. This peptide participates in the regulation of gastrointestinal motility with motilin. [1][2][3][4][5][6] Somatostatin as a gut regulatory peptide stimulates migrating motor complex (MMC)-like activity and its analogue was the first to be used therapeutically among the gastrointestinal peptides.7) Motilin, a peptide connected with somatostatin, has a powerful fundic pouch motor-stimulating activity and is one of the most important factors controlling the regular occurrence of phase 3 contractions of the MMC. 8,9) Another gut regulatory peptide, gastrin, stimulates acid secretion, and its release is mediated by various pathways such as direct stimulation of G cells, mediation by cholinergic nerves, stimulation of gastrin-releasing peptide, stomach pH, etc. 10)Vasoactive intestinal peptide (VIP) is widely distributed in the central and peripheral nervous system. This peptide has a vasodilating effect in many vascular beds, including the peripheral systemic and splanchnic beds and the cerebral arteries, and is an important neurotransmitter for the enteric nervous system. 11,12) Hange-shashin-to (EK-14), a Kampo herbal medicine, is prepared from seven crude herbs: Pinelliae Tuber, Scutellariae Radix, Zingiberis Rhizoma, Ginseng Radix, Glycyrrhizae Radix, Zizyphi Fructus, and Coptidis Rhizoma. Hangeshashin-to is frequently used for acute or chronic gastrointestinal catarrh, fermentative diarrhea, and acute gastroenteritis. This medicine has been clinically evaluated as more effective in the treatment of chronic hypofunction of the gastrointestinal system than is Rikkunshi-to in the treatment of hyperfunctioning conditions. Recently, Hange-shashin-to has been reported to be effective against diarrhea as a side effect of carcinostatic drugs. 13)Based on empirical effects of Kampo, some gut-targeting medicines have been determined to act at the gut regulatory hormone level. Ninjin-to, Rikkunshi-to, and Dai-kenchu-to affect gastrointestinal motility and based on empirical evidence their effects are assumed to be due to changes in the levels of somatostatin-, motilin-, gastrin-, or VIP-immunoreactive substances (IS) in plasma.14-18) Furthermore, abnormalities of gastrointestinal function is presumed to result from obstruction of the autonomic nervous system and changes in hormone levels. Thus we examined the plasma levels of gut regulatory peptides (somatostatin, motilin, gastrin, and VIP).Radioimmunoassays (RIA) to detect motilin have been developed by several groups using 125 I-motilin. [19][20][21] However, in terms of safety, sensitivity, and ease of handling, RIA methods are still less than satisfactory. We developed a sensitive and specific double-antibody enzyme immunoassay (EIA) for detecting motilin, using motilin-linked b-D-galactosidase as a marker antigen, a secondary antibody-coated immunoplate, and 4-methylumbelliferyl-b-D-galactopyranoside as a fluorogenic substrate.The purpose of this...

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Section: Methodsmentioning

confidence: 99%

Section: )mentioning

confidence: 99%

Hange-shashin-to Raises Levels of Somatostatin, Motilin, and Gastrin in the Plasma of Healthy Subjects.

Naito

Itoh

Yasunaga

et al. 2002

Biological & Pharmaceutical Bulletin

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“…In these studies, the dosage of motilin employed now turns out to be a pharmacological one. Another significant observation has been that motilin (Itoh et al, 1976;Lee et al, 1978) ( Lee et al, 1978) produced the plasma levels of immunoreactive motlin comparable to that observed in phase III of interdigestive myoelectric activity. Thus, this action of motilin on interdigestive myoelectric activity is probably a physiological one.…”

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confidence: 80%

Actions of Motilin on Gastrointestinal Motility and Plasma Immu.noreactive Motilin Concentration in Interdigestive and Postprandial States

Chey

Lee

1980

Endocrinol Japon

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Pharmacology of neuroendocrine peptides

Daniel

Collins

Fox

et al. 1989

Comprehensive Physiology

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The sections in this article are: Gastrin and Cholecystokinin ( CCK ) Classification of Gastrin‐ CCK Receptors Locus of Receptors Modulation of Gastrin‐ CCK Receptors Postreceptor Mechanisms Applied Pharmacology in the Gut Substance P and Tachykinins Receptors to Tachykinins Degradation of Tachykinins Actions of Tachykinins Vasoactive Intestinal Polypeptide ( VIP ) Receptors Antagonists Control of Motility Opiates Natural Opiates Receptor Types Degradation of Opiates Opiates and Guinea Pig Ileum Actions on Segments of Gastrointestinal Tract in other Species Opiates, Immune System, and Gastrointestinal Motility Rational Design of Drugs Other Peptides Motilin Somatostatin Neurotensin Gastrin‐Releasing Polypeptide/Bombesin Family Galanin

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Radioimmunoassay of motilin

Cited by 122 publications

References 25 publications

Hange-shashin-to Raises Levels of Somatostatin, Motilin, and Gastrin in the Plasma of Healthy Subjects.

Hange-shashin-to Raises Levels of Somatostatin, Motilin, and Gastrin in the Plasma of Healthy Subjects.

Actions of Motilin on Gastrointestinal Motility and Plasma Immu.noreactive Motilin Concentration in Interdigestive and Postprandial States

Pharmacology of neuroendocrine peptides

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