Radioimmunoassay of parathyroid hormone in hypercalcemic patients with malignant disease

Benson, Ralph C.; Riggs, B. Lawrence; Pickard, Barbara M.; Arnaud, Claude D.

doi:10.1016/0002-9343(74)90810-9

Cited by 152 publications

(19 citation statements)

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“…2 (top) shows the PGE level in pooled culture medium from the same experiment. (Lee and Attallah, 1975) and 1,25-(OH)2D3 (Lawson et al, 1971) are produced in normal kidney, and there is an inicidence, of uinknown frequency, of secretion of PTH by renal cortical carcinoma (Buckle, McMillan anid Mallinson, 1970;Greenberg et al, 1973 writh hypercalcaemia (Benson et al, 1974) it has beeni exclutded as a causative agent in many other hyperealcaemic patients free from skeletal metastases (Powell et al, 1973). Although cyclie AMP is involved in the action of PTH on bone (Chase and Autrbach, 1970) Prostaglandins are potenlt bone-resorbitg agents in vitro (Klein and Raisz, 1970) and are associated, probably as causative agents, with hypercalcaemia of malignancy (Tashjian et al, 1972;Powles et al, 1973;Bennett et al, 1975;Dowsett et al, 1976).…”

Section: Co-culture Experimentsmentioning

confidence: 99%

Secretion of prostaglandins as bone-resorbing agents by renal cortical carcinoma in culture

Atkins¹,

Ibbotson²,

Hillier³

et al. 1977

Br J Cancer

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Summary.-Fragments of human renal carcinoma tissue have been co-cultured with mouse calvaria. In 9/13 cases significant bone resorption occurred whilst in no case did control kidney cause significant resorption. When bone resorption did occur, it could be reduced by inclusion of indomethacin in the culture medium. In some cases when theophylline was included in culture medium to prevent cyclic AMP breakdown, there was enhancement of tumour-induced bone resorption. Control studies without tumour showed that none of the experimental treatments had a direct effect on bone. Radioimmunoassay of prostaglandin E (PGE) levels in pooled culture media showed that tumour fragments produced appreciable amounts of PGE, and that this production was lowered by indomethacin and increased by theophylline. It is concluded that the bone resorption induced by these tumours is due to a prostaglandin, and that prostaglandin production may be controlled by changes in cyclic AMP metabolism.

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Section: Co-culture Experimentsmentioning

confidence: 99%

Secretion of prostaglandins as bone-resorbing agents by renal cortical carcinoma in culture

Atkins¹,

Ibbotson²,

Hillier³

et al. 1977

Br J Cancer

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“…While Benson et al (1974) have found measurable circulating hormone in the majority of such patients this is not the experience of others Powell et al, 1973). The question whether immunoassayable circulating PTH in malignant disease reflects ectopic secretion, non-specific inter- ference in the assay, or the production of parathyroid-stimulating factors will be resolved only when assays with more clearly defined specificity can be used.…”

Section: Parathyroid Hormone Secretion In Hypercalcaemiamentioning

confidence: 96%

“…It is possible that this apparent overlap in hormone levels between normal and disease states results in part from the technical problems of measuring a peptide in the peripheral circulation which is apparently heterogeneous. Nevertheless similar results have been found in a number of laboratories using a range of antisera of varied specificity, and speculation over the origin of this problem remains (Potts et ai., 1971;O'Riordan et al, 1972;Arnaud et al, 1974).…”

Section: Parathyroid Hormone Secretion In Hypercalcaemiamentioning

confidence: 99%

Assay of Parathyroid Hormone in Human Serum and its Uses

Woodhead

Walker

1976

Ann Clin Biochem

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The assay of parathyroid hormone has contributed greatly to our understanding of calcium metabolism in health and disease. The most important clinical application of the assay is in the differential diagnosis of hypercalcaemia, which is an increasing clinical problem. PTH assays are of little or no value in the absence of other biochemical data, especially accurate determinations of plasma calcium and phosphate.

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“…This search was by and large negative, other than for some confusion caused by the original antisera used for identification of PTH by RIA, and some investigators reported that PTH-or at least PTH-like molecules-were produced by tumors associated with malignancy. [2][3][4] In retrospect, these assays may have been weakly recognizing PTH-related peptide (PTHrP), which is closely related at its N-terminal end to native PTH. The situation was clarified further in the 1980s, when it was found that patients with hypercalcemia of malignancy frequently had increased nephrogenous cAMP and, moreover, had a circulating factor that increased adenylate cyclase activity in cultured bone cells or renal membranes in much the same manner that PTH did but was clearly not PTH.…”

mentioning

confidence: 99%