Radioimmunoassay of Peripheral Plasma Testosterone in Males from Eight Species Using a Specific Antibody Without Chromatography

Falvo, Richard E.; Nalbandov, A. V.

doi:10.1210/endo-95-5-1466

Cited by 92 publications

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“…The blood plasma testosterone (T) and 5a-DIIT of adult male rabbits were 1.18 ± 0.17 and 0.42 ± 0.04 ng/ml, respec tively. These values are similar to those reported by Falvo and Nalbandov (11) and Schanbacher and Ewing (20) using Sephadex LH-20 column chromato graphic and radioimmunoassay techniques. The blood plasma concentration (mean ± SEM) of different steroids were as follows: androstenedione (A4A) 120 ± 21 pg/ml, pregnenolone (ASP) 0.55 ± 0.08 ng/ml, dehydroepiandrosterone (DHEA) 0.41 ± 0.02 ng/ml, estrone (Ei) 45.3 ± 1.7 pg/ml and estradiol-17/3 (E2) 33.1 ± 2.4 pg/ml ( fig.…”

Section: Resultssupporting

confidence: 91%

Determination of Seven Unconjugated Steroids in the Blood and Seminal Plasma of the Fertile Male Rabbit

Saksena¹,

Lau²,

Chang³

1977

Horm Res

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Seven unconjugated steroids were measured in the blood and seminal plasmas of fertile male rabbits by radioimmunoassay. The blood plasma testosterone concentration was 4-5 times that of the seminal plasma. Dehydroepiandrosterone, estrone and 17β-estradiol were found in measurable amounts in the blood plasma; however, these steroid levels were slightly lower in seminal plasma. Androstenedione and 5α-dihydrotestosterone were present in equal quantities in both the seminal and blood plasmas. By contrast, seminal plasma pregnenolone level was about twice that of the blood plasma. The determination of seminal plasma steroids may lend itself as a complementary assessment to blood steroid determinations for the evaluation of the normal function of various reproductive organs.

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Section: Resultssupporting

confidence: 91%

Determination of Seven Unconjugated Steroids in the Blood and Seminal Plasma of the Fertile Male Rabbit

Saksena¹,

Lau²,

Chang³

1977

Horm Res

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“…some workers give 0.09-4.8 ng/ml [Rivarola et al, 1968] and some others 26.2 ng/ml [Falvo and Nalbandov, 1974]. In the bull, this irregular release was also pointed out [Katongole et al, 1971].…”

Section: Indi Vidua I Variabilitymentioning

confidence: 89%

Annual Variation in the Plasma Testosterone in Microcebus murinus

Petter‐Rousseaux¹,

Picon²

1981

IJFP

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“…Hormone Assays. Plasma concentrations of LH (Niswender et al, 1970) and testosterone (Falvo and Nalbandov, 1974) were determined in duplicate 200 /A samples by previously described radioimmunoassay procedures. Sensitivities for the respective assays were 10 pg/tube for LH (LER-786-3, which has an LH biological activity of .65 • NIH-LH-S1 U/mg) and 10 pg/tube for testosterone (T).…”

Section: Methodsmentioning

confidence: 99%

Effect of Active Immunization against LHRH or LH in Boars: Reproductive Consequences and Performance Traits

Falvo

Chandrashekar

Arthur

et al. 1986

Journal of Animal Science

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Forty crossbred boars were equally divided into eight groups at birth. Four groups were immunized (200 ~g/boar) at 12 wk of age against either luteinizing hormone-releasing hormone (LHRH) conjugated to human serum globulin (LHRH-hSG) in complete Freund's adjuvant (CFA), LHRH-hSG in muramyldipeptide adjuvant (PEP), procine luteinizing hormone (LH) conjugated to hSG (pLH-hSG) in CFA or ovine LH (oLH) in CFA. Equal doses of boosters were given in either PEP or incomplete Freund's adjuvant (IFA) at 16 and 18 wk of age. Two groups of boars were immunized with either hSG + CFA or hSG + PEP (adjuvant controls). Two groups were castrated either at the time of weaning (castrate weaning) or at 16 wk when immunized boars were given their first booster injections (castrate booster). All pigs were slaughtered at 24 wk of age. Serum levels of LH and testosterone (T), LHRH or LH antibody titers, as well as testicular and accessory sex gland weights and histology were determined. By wk 16, LHRH antibody titers began to rise in those boars immunized against LHRH-hSG. Luteinizing hormone-releasing hormone antibody titers on wk 18, 20 and 22 were greater than those at wk 16. By 22 wk of age, LHRH-hSG boars had non-detectable plasma LH and T and reduced weights of testes and acessory sex glands. Boars immunized against oLH did not respond to treatment, whereas pLH-hSG boars showed a reduction in serum T levels and accessory sex gland weights. Immunization had no effect on average daily gain, hot carcass weights or loin eye area. Animals immunized against LHRH in CFA had increased lOth rib fat (P<.05) when compared with the castrated controls, adjuvant controls and the pLH-immunized boars. The incidence of boar taint was reduced (P<.05) in the LHRH-hSG and pLH-treated boars. We conclude that LHRH imrnunoneutralization resulted in castration-like carcass effects in boars but had no effect on performance characteristics, with the possible exception of backfat thickness. These data demonstrate for the first time that active immunization against LHRH is both feasible and practical as an alternative to surgical castration in boars.

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Radioimmunoassay of Peripheral Plasma Testosterone in Males from Eight Species Using a Specific Antibody Without Chromatography

Cited by 92 publications

References 0 publications

Determination of Seven Unconjugated Steroids in the Blood and Seminal Plasma of the Fertile Male Rabbit

Determination of Seven Unconjugated Steroids in the Blood and Seminal Plasma of the Fertile Male Rabbit

Annual Variation in the Plasma Testosterone in Microcebus murinus

Effect of Active Immunization against LHRH or LH in Boars: Reproductive Consequences and Performance Traits

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