Radioimmunoassay of plasma testosterone

Collins, William P.; Mansfield, M. D.; Alladina, N.S.; Sommerville, Ian F.

doi:10.1016/0022-4731(72)90080-5

Cited by 168 publications

(38 citation statements)

References 4 publications

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“…This was also seen when sulphapyridine was compared with controls 6.0 (2.5-10-5) vs [12][13][14][15][16][17][18][19][20][21][22][23][24][25] (Fig. 6).…”

Section: Ratsmentioning

confidence: 66%

Reversible male infertility due to sulphasalazine: studies in man and rat.

O’Moráin¹,

Smethurst²,

Doré³

et al. 1984

Gut

208

101

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SUMMARY Sulphasalazine treatment for inflammatory bowel disease in man causes oligospermia, reduced sperm motility and an increased proportion of abnormal forms. On withdrawal of sulphasalazine these effects are found to be reversible and 15 pregnancies occurred at a median of 2-5 months after stopping sulphasalazine therapy. Seminal plasma concentrations of acid phosphatase fructose and PGE2 as well as the hormone profiles of patients on sulphasalazine for three months were found to be within normal limits. Sulphasalazine fed to male Sprague Dawley rats caused a dose dependent and reversible infertility with a significant reduction in litter size. Rats fed the metabolite sulphapyridine also had a reduced litter size when mated, while those fed the metabolite 5'aminosalicylic acid and a polymer of 5'aminosalicylic acid did not. It seems likely that the sulphapyridine moiety of sulphasalazine is responsible for the infertility seen, the effect being mediated at a late stage in sperm maturation.Sulphasalazine has an established role in the treatment of ulcerative colitis and is increasingly used for indefinite maintenance treatment since its introduction over 40 years ago.' It has also been

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“…This was also seen when sulphapyridine was compared with controls 6.0 (2.5-10-5) vs [12][13][14][15][16][17][18][19][20][21][22][23][24][25] (Fig. 6).…”

Section: Ratsmentioning

confidence: 66%

Reversible male infertility due to sulphasalazine: studies in man and rat.

O’Moráin¹,

Smethurst²,

Doré³

et al. 1984

Gut

208

101

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“…The method used was that described by Collins, Mansfield, Alladina & Sommerville (1972). Plasma samples to which an internal standard of tritiated testosterone had been added were extracted with diethyl ether and chromatographed on a Sephadex LH-20 column.…”

Section: Introductionmentioning

confidence: 99%

Plasma FSH, LH and testosterone levels in the male rat during degeneration of the germinal epithelium caused by severe heat treatment or ligation of the vasa efferentia

Collins¹,

Collins²,

McNeilly³

et al. 1978

Reproduction

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“…(3) Hormone cmujsis Plasma testosterone was measured by the method of Collins et al (1972) except that a more specific antiserum to testosterone-3-carboxymethyl oxime-bovine serum albumin was used and the chromatographic step omitted. For the estimation of testicular testosterone the frozen biopsy specimens were individually thawed, blotted dry, weighed and then homogenised in water.…”

Section: (1) Patientsmentioning

confidence: 99%

Testicular testosterone concentration, interstitial cell density and spermatogenesis in infertile men

Hirsh¹,

Tyler

Landon³

et al. 1981

Int J of Andrology

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The concentration of testosterone was estimated in testicular biopsies taken from infertile men under general anaesthesia and was found to vary between 0.77 and 9.87 nmol/g wet tissue (median 3.4 I nmol/g). Spermatogenesis was assessed by the Johnsen scoring techniqfie hut there was no evidence of a deficiency of intratesticular testosterone bsing associated with dysfunction of the seminiferous tubules. Similarly, ther: was no direct relationship with testicular size, clinical diagnosis, or the concentration of testosterone found in peripheral plasma taken at the same time as the testicular biopsy. These negative findings were not explained hy \ariation in the volume densities of the k y d i g cells assessed in 10 biopsies selected to be representative of the r;inge of testosterone concentrations found in the entire series. A retrospective comp;irison between the concentration oi testosterone in peripheral plasma at the time of surgery and the available preoperative levels found in 10 patients provided further evidence for the previously reported lowering of testosterone levels by general anaesthesia. It is suggested that future studies of testicular androgens should be conducted on tissue obtained under local anaesthesia in order to eliminate this effect.

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Radioimmunoassay of plasma testosterone

Cited by 168 publications

References 4 publications

Reversible male infertility due to sulphasalazine: studies in man and rat.

Reversible male infertility due to sulphasalazine: studies in man and rat.

Plasma FSH, LH and testosterone levels in the male rat during degeneration of the germinal epithelium caused by severe heat treatment or ligation of the vasa efferentia

Testicular testosterone concentration, interstitial cell density and spermatogenesis in infertile men

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