Radioimmunoassay of progesterone in unextracted serum.

Haynes, S.P.; Corcoran, J. M.; Eastman, Creswell J; Doy, F.A.

doi:10.1093/clinchem/26.11.1607

Cited by 24 publications

(3 citation statements)

References 9 publications

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“…A standard ICP-OES (Perkin-Elmer, Optima 2000 DV®) analyzer system was used for the determination according to (Oser 1965). Progesterone was estimated in both plasma and milk by the radioimmunoassay (RIA) technique using the coated tube kits according to Haynes et al (1980) and Blight & White (1983). The kit was purchased from Institute of Isotopes Co. LTD. Budapest and was labelled with I 125.…”

Section: Methodsmentioning

confidence: 99%

Blood Biochemical Components and Progesterone Hormone on Day of Estrus in Crossbred Cattle in Egypt

Mourad¹

2018

JITV

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Deficiencies or excess minerals such as P, Cu, and Zn are associated with subnormal fertility and anoestrus conditions in cows. This study was conducted in a veterinary unit in Menufiya, Egypt. Eighteen head of crossbred cows were randomly selected at random at estrus time (estrus group) and as a control 14 head of crossbred cows were selected from newly-calving dams at about 6-12 hours after calving (control group). The aim of this study was to use the components of biochemistry and progesterone hormone on estrus day of crossbreeding cattle in Egypt. The information from this study will be used to confirm estrus time to improve mating percentage. In blood plasma, the concentrations of all biochemical components and progesterone concentrations in estrus animals are higher than control cows except globulin. The results showed that blood plasma from control animal obtained Mg, Ca / P ratio, Co, Cu, Zn, Se, and Mo concentration is higher than estrus cattle. On the other hand, blood plasma concentrations of Na, K, Ca, P, Mn, and Fe are higher in estrus cattle. The progesterone concentration of estrus cattle is lower during summer than in winter. In estrus cows, higher plasma macro-elements were found in winter than in summer except for K, Ca and Ca/P ratio. On the other side of estrus cattle, all trace elements of blood plasma are higher in winter than in summer except Zn, Mn, Se, and Fe.

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Section: Methodsmentioning

confidence: 99%

Blood Biochemical Components and Progesterone Hormone on Day of Estrus in Crossbred Cattle in Egypt

Mourad¹

2018

JITV

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“…Serum progesterone was estimated by Radioimmunoassay (RIA) using Coat -A count 1 125 progesterone kit (Diagnostic product corporation, Los Angeles, USA) according to Haynes et al (1980) and Kubasik et al (1984). The quantitative measurement of Estradiol in serum was done by RIA using coat-A count 1 125 Estradiol 17-β kit (Diagnostic product corporation, Los Angeles, USA) according to Xing et al (1983).…”

Section: Blood Samplesmentioning

confidence: 99%

Effect of Parity and Live Body Weight of She-Camel on Reproductive Performance, Calving Characteristics, and Blood Parameters during Post-Partum Period

El-Harairy

El-Metwaly²,

Khalaf³

2019

Journal of Animal and Poultry Production

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This study aimed to evaluate the effects of parity order (PO) and live body weight (LBW) on reproductive, calving and blood parameters of Maghrabi She-camels during post-partum period in Egypt. Post-partum 28 she-camels were divided according to LBW into four categories {400-430 (n=7), >430-460 (n=7), >460-500 (n=6) and >500-560 (n=8) kg}, and according to parity to 1 st -2 nd (n=8), 4 th (n=7) and 5 th -6 th (n=9). Results showed that number of estrus cases/animal, post-partum 1 st estrus interval (PPFEI) and cervical length increased by increasing LBW >500 kg (P≥0.05). Cervical closure time (P≥0.05) and uterine involution (P<0.05) increased by increasing LBW >430 kg. Uterine horn thickness, uterine length, cervical diameter and plasma progesterone and estrogen were not affected significantly by LBW. Estrus cases (n)/animal, cervical closure time and uterine involution increased in multi-parous than in primi-parous (P≥0.05), while cervical length increased at 5-6 parity compared with 1-4 parity (P≥0.05). The PPFEI, uterine horn thickness, uterine length, and progesterone and estrogen concentrations showed inconsistent changes in different parities (P≥0.05). Placental weight with or without fluids and calf weight at birth were not affected by weight or parity. Concentration of total proteins and their fraction as well as albumin/globulin ratio in blood plasma was not affected by weight. Glucose level reduced (P<0.05), ALP activity decreased (P≥0.05) by increasing weight. In conclusion parity and live body weight showed inconsistent trend of changes on reproductive performance, calving performance and blood biochemicals and hormonal concentrations during post-partum period of maghrabian she-camel under the Egyptian condition.

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“…Commercial ELISA relies on the use of progesterone-enzyme conjugates and these lack an efficient and convenient purification method (Mitchell et al 2004). Conventional assays involve extraction of progesterone from serum by organic solvent; however, the direct assay of progesterone in unextracted serum has also been described (Haynes et al 1980, Ratcliffe 1982, Lagana et al 1986).…”

Section: Introductionmentioning

confidence: 99%

Polish Journal of Veterinary Sciences

Rekešiūtė

Januškauskas

Šiukščius

et al. 2019

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Progesterone (P4) is responsible for the main reproduction processes. Concentration of P4 varies widely among different determination methods, and interpretation of these values may be difficult. The objective of the current study was to assess the agreement of three different enzyme immunoassays (ELISA) in relation to radioimmunoassay (RIA) of P4 concentration assessment of beef cow serum samples.Samples were collected randomly considering high (pregnant cows) and low (non-pregnant cows) P4 concentrations. Depending on the P4 assessment method, four groups were created as follows: Group 1 -direct samples assessed by ELISA, Group 2 -extracted samples assessed by ELISA, Group 3 -samples assessed by automated ELISA, and Group 4 -samples assessed by RIA.The mean progesterone concentration was 4.50 ng/mL, 1.24 ng/mL, 4.07 ng/mL and 4.39 ng/mL from Group 1 to Group 4, respectively. The mean difference (MD) between Group 1, Group 2 and Group 3 individually compared with Group 4 was −0.10 ± 1.24 ng/mL, 3.15 ± 3.58 ng/mL and 0.33 ± 1.42 ng/mL, and the 95% confidence interval (CI) for the differences (s) was from −0.99 to 0.78 ng/mL, from 0.59 to 5.71 ng/mL, and from −0.69 to 1.34 ng/mL, respectively. The confidence interval for the lower and upper limit of the agreement ranged from −4.12 to −1.05 ng/mL and from 0.84 to 3.91 ng/mL between Group 1 and Group 4, from −8.45 to 0.42 ng/ mL and from 5.88 to 14.75 ng/mL between Group 2 and Group 4, from −4.29 to −0.76 ng/mL, and from 1.41 to 4.94 ng/mL between Group 3 and Group 4.Our findings show that the best agreement with RIA was observed for Group 1 and Group 3, while the agreement in the extraction method was least accurate.

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Radioimmunoassay of progesterone in unextracted serum.

Cited by 24 publications

References 9 publications

Blood Biochemical Components and Progesterone Hormone on Day of Estrus in Crossbred Cattle in Egypt

Blood Biochemical Components and Progesterone Hormone on Day of Estrus in Crossbred Cattle in Egypt

Effect of Parity and Live Body Weight of She-Camel on Reproductive Performance, Calving Characteristics, and Blood Parameters during Post-Partum Period

Polish Journal of Veterinary Sciences

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