Radioimmunoassay of prostaglandins

Granström, Elisabeth

doi:10.1016/s0090-6980(78)80002-1

Cited by 64 publications

(14 citation statements)

References 141 publications

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“…Samples (0.2 ml and 0.1 ml) were run in an adapted radioimmunoassay according to Granstrom & Kindahl (1978), using an antiserum which we produced in a final dilution of 1/17500. Cross reactivities at 50% of the binding curve were:…”

Section: Radioimmunoassay Of Pgf2amentioning

confidence: 99%

Prostaglandin F2α in benign and malignant breast tumours

Vergote¹,

Laekeman²,

Keersmaekers³

et al. 1985

Br J Cancer

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Prostaglandins (PGs), especially of the E-series, have been shown to be elevated in a large number of human and experimental tumours. Special efforts have been made to investigate the features and the role of PG-synthesis in human breast cancer (Bennett et al., 1975;Powles et al., 1976; Kibbey et al., 1980;Greaves et al., 1980;Malachi et al., 1981;Campbell et al., 1983).A considerable volume of research on the mechanisms of action of PGs in a wide variety of cells and tissues, indicates that PGs are possibly involved in tumour initiation, tumour promotion, cell proliferation and differentiation, the immune response, tumour metastasis, osteolysis and hypercalcaemia (Karmali, 1980;Honn et al., 1981a;Droller, 1981;Goodwin, 1981).The present study was designed to describe the basic features of PGF2X, production in benign and malignant breast tumours. Normal glandular breast tissues were used as controls. We determined PGF2<,, because this product is more stable than Brune et al., 1978). Therefore the numbers of host-derived cells and the amount of necrosis were evaluated by means of quantitative microscopy. The epithelial cellularity was also evaluated by morphometric determination of the mean nuclear density and mean nuclear area. Materials and methodsThere were 165 specimens from 106 patients who underwent surgery for a breast lump. Each C) The Macmillan Press Ltd., 1985

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Section: Radioimmunoassay Of Pgf2amentioning

confidence: 99%

Prostaglandin F2α in benign and malignant breast tumours

Vergote¹,

Laekeman²,

Keersmaekers³

et al. 1985

Br J Cancer

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“…The radioimmunoassay of TXB2 was performed directly on thawed aliquots essentially as described by Granstrijm and Kindahl (8). The antiserum to TXBl was purchased from Seragen (Boston, MA) and used according to the manufacturers' directions except that IgG Sorb (The Enzyme Center, Cambridge, MA) was used in the final antigen-antibody complex precipitation (9).…”

Section: Methodsmentioning

confidence: 99%

Differences in Thromboxane Production between Neonatal and Adult Platelets in Response to Arachidonic Acid and Epinephrine

et al. 1984

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SummaryIn this study, we have investigated the possible role of the proaggregatory arachidonic acid (AA) metabolite thromboxane, in the impaired function of neonatal platelets. In platelet-rich plasma thromboxane production (measured by radioimmunoassay of thromboxane B2) was not different between neonates and adults when stimulated by thrombin (at 0.1 or 1.0 U/ml) or collagen (70 pg/ml) although neonatal platelets produced decreased thromboxane (TBXZ) postepinephrine stimulation. In response to 1 U/ml thrombin, adult and neonatal platelet-rich This elevated thromboxane production was not, however, inherent in neonatal platelets since when washed platelets were studied, results were reversed. Adult platelets produced more thromboxane at all doses of AA evaluated. These results suggest that the elevated response to exogenous AA observed in neonatal platelet-rich plasma results from as yet undetermined plasma factors. The reported deficiencies in platelet function in the newborn clearly do not result from deficient thromboxane production poststimulation with the physiologic aggregating agents collagen and thrombin. Moreover, our study introduces a new and possibly important difference between adult and neonatal plasma, namely, the differential response to exogenous arachidonic acid.Abbreviations AA, arachidonic acid TXB2, thromboxane Bz PRP, platelet-rich plasma

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“…Ice was regularly added to the bath of fluid of the sonification apparatus to keep the temperature below 10°C. The supernatant was separated from the tissue after cen trifugation at 10,000 g. Samples were run in an adapted radio-immunoassay according to Granstrom and Kindahl [10], using our own antisera. Radioactiv ity was counted in a Packard 460 scintillation count er.…”

Section: Radio-immunoassay O F 6 -Keto -P G F a N D Txb> And Histologmentioning

confidence: 99%

Prostacyclin/Thromboxane Ratio in Human Breast Cancer

et al. 1991

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In this prospective follow-up study the prognostic value of the tumor prostacyclin/thromboxane ratio in human breast carcinoma was investigated. The stable degradation products of prostacyclin and thromboxane (6-keto-PGF_1α and TXB₂, respectively), were measured by radio-immunoassay in homogenized primary tumours from 29 patients with primary non-metastatic breast cancer. The median follow-up was 43 months (range 24–58 months). Patients with recurrent disease or patients who died of breast cancer had a significantly higher 6-keto-PGF_1α/TXB₂ ratio than the disease-free survivors (p = 0.018 and p = 0.047, respectively). There was no significant difference in the 6-keto-PGF_1α and TXB₂ levels. These data indicate that the prostacyclin/thromboxane balance in the tumour might be a prognostic factor in breast cancer. Prostanoids may contribute to metastasis in breast cancer, but the problem is complex because the different prostaglandins have numerous actions that may produce both undesirable and desirable effects.

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Radioimmunoassay of prostaglandins

Cited by 64 publications

References 141 publications

Prostaglandin F2α in benign and malignant breast tumours

Prostaglandin F2α in benign and malignant breast tumours

Differences in Thromboxane Production between Neonatal and Adult Platelets in Response to Arachidonic Acid and Epinephrine

Prostacyclin/Thromboxane Ratio in Human Breast Cancer

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