Radioimmunological Quantitation of Human Group-II Pepsinogens

Matzku, S.; Zoller, M.; Rapp, W.

doi:10.1159/000198180

Cited by 11 publications

(2 citation statements)

References 9 publications

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“…With respect to serum pepsinogen C, the picture is more complicated; some authors described a significantly higher serum concentration in males than in females (10,18), while, in agreement with our findings, a more recent study found no significant difference (9).…”

Section: Discussionsupporting

confidence: 90%

Radioimmunoassay of Human Pepsinogen A and Pepsinogen C

Biemond¹,

Jansen²,

Crobach³

et al. 1989

Clinical Chemistry and Laboratory Medicine

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Summary:We describe the development of radioimmunoassays to measure both human pepsinogen A and pepsinogen C concentrations in serum. The antibodies were raised in goats by immunization with purified pepsinogen A or C. The affinity constants of the respective antibodies were 20 · l O 10 1/mol and 7 · l O 10 1/mol.Pepsinogens A and C were labeled with Na 125I by the chloramine T method. The binding between labels and antibodies was inhibited by 0.50 at 0.82 ng pepsinogen A per tube and 2.1 ng pepsinogen C per tube. The detection limits of the assay of pepsinogen A and C were 0.12 μg/l and 1.8 μg/l, respectively.Pepsinogen A and C were purified and added to a patient serum, showing a good recovery in the radioimmunoassays. Serial dilution of another patient serum, which contained a high concentration of both antigens, showed curves parallel to the Standard curves. The intra-and interassay variations of these radioimmunoassays were evaluated. The intra-assay coefficients of Variation for pepsinogen A were found to vary from 0.03 to 0.102 at concentrations in serum in the normal r nge, while the inter-assay coefficient of Variation ranged from 0.118 to 0.194 at the same concentrations in serum. For the pepsinogen C radioimmunoassay we found intra-assay coefficients of Variation between 0.126 and 0.147 at concentrations in serum in the normal r nge, while the inter-assay coefficient of Variation ranged from 0.174 to 0.325 for the same sera.In 201 blood donors we found a me n serum concentration of pepsinogen A of 59 μg/l and a mean serum concentration of pepsinogen C of 15 μg/l. There was a significant relationship between these values (r = 0.779, p < 0.001).The concentrations of both pepsinogens were increased in 10 patients with duodenal ulcer (132 and 67 μg/l, respectively). Serum pepsinogen A was decreased in 14 patient« with partial gastrectomy (34 μg/l) and in 6 with achlorhydria (9 μg/l), accompanied by raised (34 μg/l) and!inormal (20 μg/l) mean pepsinogen C concentrations in the respective groups. In 4 patients with a total gastrectomy the mean serum concentration of both pepsinogen A and C reached the zero level (5 and 4 μg/l, respectively). These results not only underline the accuracy of the measurements but also point to their clinical relevance. ntro uction. § secrete^ by f un( jj c , pyloric and proximal duodenal Pepsinogen A and C are the proenzymes of pepsin A mucosa (1). Exocrine-secreted pepsinogens function and pepsin C, respectively, which belong to the group s zymogens for the main proteolytic activity of gasof gastric acid aspartic endoproteiiiases. Pepsinogen tric juice. There is also a small endocrine excretion of A is secreted by fundic mucosa, while pepsinogen C pepsinogens without a known function, but by exploiting this phenomenon, we can gain Informatioñ on the condition of the gastric mucosa by measurePepsin* *Α; EC 3 4.23.1 ment of the serum concentration of pepsinogen A and Pepsin C; gastricsin; EC 3.4.23.3 pepsinogen C (2, 3).

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Section: Discussionsupporting

confidence: 90%

Radioimmunoassay of Human Pepsinogen A and Pepsinogen C

Biemond¹,

Jansen²,

Crobach³

et al. 1989

Clinical Chemistry and Laboratory Medicine

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“…The serum PG I and PG II levels of healthy males (females) are 117.2 (103.4) ng/mL and 20.3 (15.5) ng/mL, respectively. 10,11 Decreased serum PG I levels and PG I : II ratios are correlated with the extent of chronic gastritis and are significantly lower than in normal controls. 12 The development of assays for serum PG has therefore enabled non‐invasive evaluation of chronic gastritis and severe atrophy.…”

Section: Discussionmentioning

confidence: 99%

A case of gastric cancer with high pepsinogen II levels in both serum and ascites

Muto

Saito

Koike

2000

Digestive Endoscopy

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The first case of gastric cancer in which pepsinogen (PG) II levels were found to be extremely high not only in the serum but also in the ascites, with values of 603 ng/mL and 1910 ng/mL, respectively, is reported. Pepsinogen I and PG II are normally secreted into the gastric lumen and only 1% of the amount secreted enters the circulation. Although gastric cancer cells are found to produce PG II more often than PG I, elevated PG values in serum are extremely rare, and only one case has ever been reported. That patient had extremely high serum levels of PG I and PG II at the time of gastric cancer relapse. Pepsinogen has never been reported in the ascites, and thus nothing is known about the mechanism of entry into the ascites. In this case report, we postulate two mechanisms to explain the increased PG II in the ascites: (i) a high level of serum PG II infiltrated the ascites and caused elevation of PG II in the ascites; or (ii) disseminated cancer cells directly produced PG II and it elevated PG II levels in the ascites.

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