Radioimmuoassay for aldosterone and normal values under various physiological conditions

Nowaczynski, W.; Sasaki, C.; Genest, Jacques

doi:10.1016/0022-4731(74)90118-6

Cited by 58 publications

(7 citation statements)

References 13 publications

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“…The PA (pmol/L) was assayed by radioimmunoassay after extraction from 2 ml of plasma with dichloromethane. 10 The PNE (nmol/L) was measured by the radioenzymatic technique described by Peuler and Johnson" with minor modifications in the composition of the incubation mixture. Rat liver catechol-o-methyl-transferase was isolated by ammonium sulfate protein fractionation.…”

Section: Methodsmentioning

confidence: 99%

Sodium balance in renal failure. A comparison of patients with normal subjects under extremes of sodium intake.

Koomans¹,

Roos²,

Mees³

et al. 1985

Hypertension

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SUMMARY To gain insight into the factors involved in the maintenance of sodium balance in patients with chronic renal failure, we studied 10 patients with a creatinine clearance of 11.5 ± 4.0 ml/min after equilibrium on 20 and 120 mEq of sodium per day. The measurements included blood pressure, plasma volume, blood volume, extracellular fluid volume, plasma renin activity, plasma aldosterone, and plasma norepinephrine. For comparison, eight normal volunteers were studied after equilibration on 20, 200, and 1128 mEq of sodium per day. The latter intake was chosen to match the high sodium intake per residual renal function in the patients. In the patients, equilibrium after raised sodium intake was accompanied by a marked increase in blood pressure and blood volume, a moderate fall in plasma renin activity and levels of aldosterone and norepinephrine, and only little expansion of the interstitial space. The 24-hour creatinine clearance rose by 21.2 ± 7.2%. Fractional sodium excretion (x 100%) was 5.3 ± 0.8% during the 120 mEq sodium diet. In the normal volunteers, increasing the sodium intake from 20 to 1128 mEq/day evoked no consistent change in blood pressure but caused a comparable rise in blood volume, considerable suppression of plasma renin activity, aldosterone, and norepinephrine, and a much larger increase in interstitial volume. Their creatinine clearance had risen by 22.4 ± 6.5%, and their fractional sodium excretion during the 1128 mEq sodium intake was 3.9 ± 0.2%. These results suggest that in normal subjects the maintenance of sodium balance over a wide range of sodium intake is particularly dependent on variations of neurohumoral influences on the kidney, while in patients with renal failure relatively large variations in arterial pressure are necessary as well. The marked difference in amount and distribution of the retained volume may be explained by a vasoconstrictive reaction to sodium loading in the patient group. (Hypertension 7: 714-721, 1985) KEY WORDS volume sodium loading renin • aldosterone hypertension • norepinephrine blood volume extracellular fluid

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Section: Methodsmentioning

confidence: 99%

Sodium balance in renal failure. A comparison of patients with normal subjects under extremes of sodium intake.

Koomans¹,

Roos²,

Mees³

et al. 1985

Hypertension

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“…PRA (fm ol/l/s) was determined radioimmunologically according to a modification of the method described by Haber et al [4] (incubation of undiluted plasma for I h at 37 C and pH 5.6, followed by deproteinization of the plasma with 4 N ammonia/acetone 1:9). PAC (pmol/1) was assayed by radioimmunoassay after extraction of 2.0 ml plasma with dichloromethane and purification on a Sephadex LH 20 column ( 1.0 x 50 cm) with the radioassay buffer as the mobile phase, accor ding to Nowaczynskiet al [5]. Correlation coefficients were calcula ted by the method of least squares.…”

Section: Methodsmentioning

confidence: 99%

Relation between Plasma Aldosterone Concentration and Renal Handling of Sodium and Potassium, in Particular in Patients with Chronic Renal Failure

Hené¹,

Koomans²,

Boer³

et al. 1984

Nephron

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In normal subjects a relation was found between the log plasma aldosterone concentration (PAC) and the ratio of renal potassium excretion (U_ĸ) and sodium delivery to the sodium-potassium exchange site (U_Na+ĸ). This relationship was independent of plasma renin activity (PRA). On these grounds, the U_ĸ/U_Na+ĸ ratio disturbance was considered to be a function of the PAC, and this relation was considered to reflect an altered sensitivity of the distal tubule to aldosterone. In several pathological conditions involving the kidney, the relation between the PAC and U_ĸ/U_Na+ĸ remained normal while the glomerular filtration rate was within normal limits. Under these conditions, however, the serum potassium concentration had some influence on this relation, in that a low potassium concentration was accompanied by an elevated (though still normal) PAC relative to the U_ĸ/U_Na+ĸ ratio. The relation was completely abolished when the effect of endogenous aldosterone was impaired by chronic spironolactone administration in patients with essential hypertension. Analysis to these relations in chronic renal disease made it possible to classify them according to the pathophysiological disturbance in question. We conclude that determination of the U_ĸ/U_Na+ĸ ratio and its relation to the PAC, PRA, and serum potassium level is very useful for the analysis of disorders of potassium metabolism.

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“…Compared with other purification methods, such as paper and thin-layer chromatography, Downloaded by [New York University] at 22:36 24 June 2016 other radioimmunological methods (3,8,11,15,16,18,25). There was no difference between males and females.…”

Section: Discussiolnmentioning

confidence: 98%

Evaluation of Radioimmunological Methods for Assay of Plasma and Urinary Aldosterone

Pakarinen

Koskinen

Adlercreutz

1976

Scandinavian Journal of Clinical and Laboratory Investigation

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Two radioimmunological methods for assay of plasma and urinary aldosterone were carefully evaluated. In the plasma method a radioimmunoassay is preceded by chromatography on a Sephadex LH-20 column. The method for urine includes a preextraction, hydrolysis of the acid-labile conjugates of aldosterone, and a radioimmunoassay. Both methods fulfill the criteria of reliability and are suitable for both routine and demanding research assays. The plasma method, using columns of double length, is also applicable to analysis of aldosterone in plasma of newborn children, and pregnant females and in cord plasma. The concentration of plasma aldosterone in healthy subjects on an ad lib salt diet was 162 +/- 93 (S.D.) pmol/1 in the supine position and 312 +/- 217 (S.D.) pmol/1 upright. The urinary excretion of aldosterone in healthy subjects was 28.3 +/- 16.7 (S.D.) nmol/24 h.

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Radioimmuoassay for aldosterone and normal values under various physiological conditions

Cited by 58 publications

References 13 publications

Sodium balance in renal failure. A comparison of patients with normal subjects under extremes of sodium intake.

Sodium balance in renal failure. A comparison of patients with normal subjects under extremes of sodium intake.

Relation between Plasma Aldosterone Concentration and Renal Handling of Sodium and Potassium, in Particular in Patients with Chronic Renal Failure

Evaluation of Radioimmunological Methods for Assay of Plasma and Urinary Aldosterone

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