2009
DOI: 10.1055/s-0029-1210932
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Radioiodination of Peptide Hormones and Immunoglobuline Preparations: Comparison of the Chloramine T and lodogen Method*)

Abstract: Following optimization of the reaction conditions, e.g. concentration of oxidizing agents, reaction time, volume of reaction mixture, and pH, chloramine T and the new iodination reagent, Iodogen, were compared for their effectiveness in radioiodination of insulin, glucagon, human growth hormone (hGH), and rabbit anti-mouse IgG. The radioactive peptide hormones prepared were analyzed for the presence of aggregate and breakdown products by polyacrylamide gel electrophoresis (PAGE) at pH 8.9, the rabbit anti-mous… Show more

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Cited by 16 publications
(7 citation statements)
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“…Administration occurred within 2 hours after labeling. The chloramine-T and Iodogen methods have been shown to yield comparable results for the radioiodination of whole mouse IgGs (37). In our hands, radioiodination via chloramine-T using a reductant to stop reactions consistently yielded high radioisotope incorporation rates without compromising immunoreactivity of the L19SIP antibodies.…”
Section: L19sip Antibody and Radiolabelingmentioning
confidence: 60%
“…Administration occurred within 2 hours after labeling. The chloramine-T and Iodogen methods have been shown to yield comparable results for the radioiodination of whole mouse IgGs (37). In our hands, radioiodination via chloramine-T using a reductant to stop reactions consistently yielded high radioisotope incorporation rates without compromising immunoreactivity of the L19SIP antibodies.…”
Section: L19sip Antibody and Radiolabelingmentioning
confidence: 60%
“…Antibody 27.F7 was labeled with Na- 125 I (specific activity 17.4 mCi/mg) (New England Nuclear, MA, USA) using Iodogen as previously described [ 30 ]. The resulting specific activity of 125 I-27.F7 was (100 mCi/mmol).…”
Section: Methodsmentioning
confidence: 99%
“…A commercial antibody to porcine insulin raised in guinea pig (Sigma, St. Louis, MO) was used as the primary antibody, and the secondary antibody was an anti-guinea pig IgG raised in horses. Porcine insulin was iodinated by the chloramine T method [26]. The assay buffer was 0.025 M barbital buffer (0.01% diethylbarbiturate, 0.05% sodium diethylbarbiturate) containing bovine serum albumin (3%; Sigma).…”
Section: Blood Sampling and Assaysmentioning
confidence: 99%