Radiolabelled granulocytes in inflammatory bowel disease

Becker, Wolfgang; Fischbach, Wolfgang; Weppler, M.; Mosl, Beate; Jacoby, Georg E.; Börner, W.

doi:10.1097/00006231-198810000-00004

Cited by 44 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Barium small bowel series show anatomic lesions but are not a measure of inflammation. It has been suggested that fecal excretion of indium-11 ( 11 In)-labeled granulocytes should be used for Crohn disease (5), but the accepted best measure in routine pediatric practice is technetium-99-labeled ( 99 Tc) white cell scanning. However, these investigations are invasive, have inherent risks, and cannot be repeated frequently in children.…”

mentioning

confidence: 99%

Fecal Calprotectin: Validation as a Noninvasive Measure of Bowel Inflammation in Childhood Inflammatory Bowel Disease

Bunn,

Bisset,

Main

et al. 2001

J. pediatr. gastroenterol. nutr.

View full text Add to dashboard Cite

BackgroundCalprotectin is an abundant neutrophil protein, which is extremely stable in feces. This study aimed to validate fecal calprotectin as a marker of bowel inflammation against invasive measures in children with inflammatory bowel disease (IBD), including colitis and small bowel Crohn disease.MethodsFecal calprotectin was measured using a simple enzyme‐linked immunosorbent assay in 36 spot stool samples from 22 children before colonoscopy and from 14 children before technetium‐99 ( 99 Tc) scanning. Using standard scoring systems, the severity of inflammation was assessed macroscopically and histologically at six standard sites in those who underwent colonoscopy and also at six standard sites in those who underwent 99 Tc scanning. The subscores from each site were summated to give combined severity and extent scores for macroscopic and for histologic inflammation in the group undergoing colonoscopy and total inflammation in the group undergoing 99 Tc scanning.ResultsIn the 22 children who underwent colonoscopy, median fecal calprotectin was 4.9 mg/L (0.1–272.5 mg/L) (range). Disease groups included six normal cases, nine ulcerative colitis cases, two isolated Crohn colitis cases, two indeterminate colitis cases, and three allergic colitis cases. Fecal calprotectin correlated closely with colonic macroscopic inflammation (r = 0.75, P < 0.001) and histologic inflammation (r = 0.85, P < 0.001). Of the 14 children undergoing 99 Tc scanning, 10 had Crohn disease, 3 had ulcerative colitis, and 1 had allergic colitis. Median fecal calprotectin was 9.1 mg/L (0.3–141.7 mg/L), and this correlated closely with the 99 Tc scanning score (r = 0.80, P = 0.001).ConclusionFecal calprotectin correlates closely with the best invasive measures of colonic and small bowel inflammation in childhood inflammatory bowel disease. As a sensitive objective measure of bowel inflammation that is risk‐free and noninvasive, fecal calprotectin lends itself particularly to the monitoring of and assessment of therapeutic interventions in children with inflammatory bowel disease.

show abstract

mentioning

confidence: 99%