Background: Detail of the deep lymphatic anatomy of the foot remains the least described in medical literature. Methods: Eight feet were harvested from four unembalmed human cadavers amputating above the ankle. A small amount of 6% hydrogen peroxide was used to find the deep lymphatic vessels around the dorsal and plantar metatarsal vessels. A fine needle was inserted into vessels and injected with a barium sulphate compound. Each specimen was dissected, photographed, and radiographed to demonstrate the deep lymphatic distribution of the foot. Results: Deep collecting lymph vessels of the foot were found in the deep tissue of the dorsal and sole of the foot, they were: Dorsal metatarsal lymph vessel (DMLV); Lateral tarsal lymph vessel (LTLV); Central tarsal lymph vessel (CTLV); Medial tarsal lymph vessel (MTLV); Dorsalis pedis lymph vessel (DPLV); Anterior lateral malleolus lymph vessel (ALMLV); Plantar metatarsal lymph vessel (PMLV); Deep plantar arch lymph vessel (DPALV); Lateral plantar lymph vessel (LPLV); Medial plantar lymph vessel (MPLV); Medial calcaneus lymph vessel (MCLV); Medial malleolus perforating lymph vessel (MMPLV). Each vessel ran concentrically, then merged successively and finally drained into the anterior or posterior tibial lymph vessels. Conclusion: The deep lymphatic distribution of the foot has been presented. The results will provide an anatomical basis for clinical management, educational reference, and scientific research.