This paper presents the initial vicarious radiometric calibration results for Landsat 9 OLI using a combination of ground-based techniques and test sites located in Nevada, California, and South Dakota, USA. The field data collection methods include the traditional reflectance-based approach and the automated Radiometric Calibration Test Site (RadCaTS). The results for top-of-atmosphere spectral radiance show an average ratio (OLI/ground measurements) of 1.03, 1.01, 1.00, 1.02, 1.02, 1.01, 0.98, and 1.01 for Landsat 9 OLI bands 1–8, which is within the design specification of ±5% for spectral radiance. The results for top-of-atmosphere reflectance show an average ratio (OLI/ground measurements) of 0.99, 0.99, 1.00, 1.02, 1.01, 1.02, 1.00, and 1.00 for Landsat 9 OLI bands 1–8, which is within the design specification of ±3% for top-of-atmosphere reflectance.