Radionuclide speciation: A key point in the field of nuclear toxicology studies

Bresson, Carole; Ansoborlo, É.; Vidaud, Claude

doi:10.1039/c0ja00223b

Cited by 36 publications

(41 citation statements)

References 108 publications

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“…The composition of the exposure solution influences uranium speciation and therefore its bioavailability. In simulated biological fluids and culture media at physiological pH conditions (around 7.5), uranium is typically found under different complexes of hexavalent uranyl ion (UO 2 2+ ) and CO 3 2-ions (40)(41)(42), which are the most common species found in human biological fluids. We developed a two-step protocol for the preparation of the exposure solution, consisting in the dilution of a natural uranium stock solution in a buffer, and the further dilution of this intermediate solution in the culture medium (SI Materials and Methods).…”

Section: Resultsmentioning

confidence: 99%

Evidence of isotopic fractionation of natural uranium in cultured human cells

Paredes

Avazeri

Malard

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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The study of the isotopic fractionation of endogen elements and toxic heavy metals in living organisms for biomedical applications, and for metabolic and toxicological studies, is a cutting-edge research topic. This paper shows that human neuroblastoma cells incorporated small amounts of uranium (U) after exposure to 10 μM natural U, with preferential uptake of the 235 U isotope with regard to 238 U. Efforts were made to develop and then validate a procedure for highly accurate n( 238 U)/n( 235 U) determinations in microsamples of cells. We found that intracellular U is enriched in 235 U by 0.38 ± 0.13‰ (2σ, n = 7) relative to the exposure solutions. These in vitro experiments provide clues for the identification of biological processes responsible for uranium isotopic fractionation and link them to potential U incorporation pathways into neuronal cells. Suggested incorporation processes are a kinetically controlled process, such as facilitated transmembrane diffusion, and the uptake through a highaffinity uranium transport protein involving the modification of the uranyl (UO 2 2+ ) coordination sphere. These findings open perspectives on the use of isotopic fractionation of metals in cellular models, offering a probe to track uptake/transport pathways and to help decipher associated cellular metabolic processes.isotopic fractionation | uranium | neuronal cells | analytical procedure development | microsamples

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Section: Resultsmentioning

confidence: 99%

Evidence of isotopic fractionation of natural uranium in cultured human cells

Paredes

Avazeri

Malard

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

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“…In fact ELI designates a family of indicators. Among them, one of the most pertinent for the study of the bonding in molecular systems is the ELI-D, symbol Υ D (8). ELI-D can be seen as being proportional to the charge that is needed to form an electron pair.…”

Section: Topological Analysis Of the Electron Densitymentioning

confidence: 99%

“…A detailed description of the formulation is available in references [82,[87][88][89]. In brief, Υ D (r) can be expressed as the product of the electron density with the pair-volume function (8):…”

Section: Topological Analysis Of the Electron Densitymentioning

confidence: 99%

“…The areas of study have expanded in recent years with applications in the fields of nuclear toxicology [3][4][5][6], cancer therapy (e.g., the radioisotope 225 Ac of actinium is an alpha emitter useful for targeted radiotherapy [7]), analytical chemistry [8], and in basic research in structural chemistry and reaction mechanisms [9]. All these applications have in common the need to develop pre-organised chelating agents.…”

Section: Introductionmentioning

confidence: 99%

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Electronic structure theory to decipher the chemical bonding in actinide systems

Dognon

2017

Coordination Chemistry Reviews

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International audienceThe chemical bonding in actinide compounds is usually analysed by inspecting the shape and the occupation of the orbitals or by calculating bond orders which are based on orbital overlap and occupation numbers. However, this may not give a definite answer because the choice of the partitioning method may strongly influence the result possibly leading to qualitatively different answers. In this review, we summarized the state-of-the-art of methods dedicated to the theoretical characterisation of bonding including charge, orbital, quantum chemical topology and energy decomposition analyses. This review is not exhaustive but aims to highlight some of the ways opened up by recent methodological developments. Various examples have been chosen to illustrate this progress

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“…Whereas chemical uranium speciation in water column correlated with its bioavailability is quite well studied by modelling, its speciation in aquatic organisms is poorly described (Bresson et al, 2011) despite a significant knowledge of coordination chemistry and affinity for proteins (Ansoborlo et al, 2006;Michon et al, 2010). In rat kidney uranium was mainly detected (after exposure) bound to acidic proteins (Frelon et al, 2009).…”

Section: Introductionmentioning

confidence: 97%