The first data obtained in the isotope hydrogeochemical studies of natural waters in the Verkh-Tula settlement are presented in the work. The composition of these waters is mainly HCO Na-Mg-Ca with TDS varying from 542 to 731 mg/dm, and silicon content 0.46 to 7.04 mg/dm. The geochemical parameters of the medium vary from reductive to oxidative with Eh -157.4 - +231, weakly alkaline pH (7,4 - 8,1) and О 0.29 - 5.52 mg/dm3. It was established that δD and δ18O of surface and ground waters differ from each other substantially and vary from -105 to -126 ‰ and from -13.2 to -16.3 ‰ - for the former, and from -136 to -138 ‰, from -18.3 to -18.8 ‰ - for the latter. According to the data obtained, for the majority of groundwaters, the time of water residence in the aquifer is not less than 5 years, and their feeding is independent of local surface waters. The isotope composition of water-dissolved carbon (δС from -14.3 to -12.5 ‰) points to the biogenic origin of carbon dioxide participating in carbonate-silicate weathering.