Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach

Bansal, Prateek; Paul, Piya; Kunwar, Amit; Jayakumar, S.; Nayak, Pawan G.; Priyadarsini, K. Indira; Unnikrishnan, M.K.

doi:10.1016/j.jff.2012.06.010

Cited by 26 publications

(10 citation statements)

References 34 publications

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“…In our study, we found decreased levels of reduced glutathione in liver tissue homogenates when rats were exposed to sublethal dose of X-rays (4 Gy), when animals were dissected 24 h post irradiation. This result is in accordance with an earlier study; where mice exposed to γ-radiation displayed reduction in reduced glutathione levels in liver homogenates when dissected 24 h post irradiation( 28 ). Pretreatment of P. americana showed significant increase in the glutathione levels when compared to irradiated group ( P < 0.05).…”

Section: Discussionsupporting

confidence: 93%

Anticlastogenic, radiation antagonistic, and anti-inflammatory activities of Persea americana in albino Wistar rat model

et al. 2017

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The present study was undertaken to evaluate the anti-inflammatory activity and antigenotoxic effect of hydroalcoholic leaf extract of Persea americana (P. americana) in albino Wistar rats against whole body X-ray irradiation. Rats were orally administered with (25, 50, 100, 200, and 400 mg/kg body weight) of P. americana leaf extract for five days. On the fifth day after last administration, animals were exposed to whole body X-rays of 8 Gy. Based on Kaplan Meier’s survival analysis, 100 mg/kg body weight was selected as an optimum dose for radioprotection. The radioprotective potential was analysed by bone marrow micronucleus test and comet assay in peripheral blood lymphocytes. Biochemical estimations were performed in liver tissue homogenates. DNA damage indicators analysed through comet assay displayed significant reduction in olive tail movement (P < 0.01), percentage DNA in tail (P < 0.05) and tail length (P < 0.001) in pretreated group when compared to radiation group. P. americana leaf extract restored the levels of reduced glutathione, catalase, and reduced the levels of lipid peroxidation, protein carbonyls, and cyclooxygenase-2 levels in liver homogenates of pre-treated group. Decrease in micronucleated polychromatic erythrocytes (P < 0.05) was witnessed in P. americana pretreated group when compared to radiation control. Pretreatment also resulted in the increase of animal survival with dose reduction factor of 1.28. Our findings for the first time demonstrated that P. americana offers significant protection to rats from whole body exposure to X-rays and helps in antagonising the radiation effects, thereby combating acute radiation induced damage in living systems.

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Section: Discussionsupporting

confidence: 93%

Anticlastogenic, radiation antagonistic, and anti-inflammatory activities of Persea americana in albino Wistar rat model

et al. 2017

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“…Quercetin has proved its beneficial role as radioprotector by reducing genotoxicity in mice exposed to ionizing radiation of 4 Gy (Benkovic et al 2009 ). Quercetin-3- O -rutinoside, a flavonoid restored intestinal damage, reduced apoptosis in splenocytes and reduced genotoxicity significantly in mice exposed to whole-body γ-radiation (Bansal et al 2012 ). Flavonoids such as quercetin and rutin play a vital role in ameliorating radiation induced damage.…”

Section: Discussionmentioning

confidence: 99%

Radiation mitigating activities of Psidium guajava L. against whole-body X-ray-induced damages in albino Wistar rat model

et al. 2020

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In the present study, we investigated radiation mitigating activities of Psidium guajava L. (P. guajava) against whole-body X- ray induced damages in albino Wistar rat model. The animals were orally administered with 200 mg/kg bodyweight of hydroalcoholic leaf extract of P. guajava for five consecutive days and on the fifth day, after the last dose of extract administration, animals were exposed to 4 Gy of X-rays. Rats were sacrificed 24 h post X–ray irradiation. The radiomitigating activity of the herb extract was assessed by micronucleus assay, histopathology of the small intestine and hematological parameters. Hepatic cyclooxygenase–2 (COX-2), interleukin–6 (IL-6) and interleukin –10 (IL-10) levels were assayed to validate the anti-inflammatory property. Biochemical estimations were also performed in RBC lysates to corroborate antioxidant properties in the leaf extract. HPLC analysis of crude extract confirmed the presence of standard flavonoid quercetin. Our results indicated that radiation elevated COX-2, IL-6 and decreased IL-10 levels and also induced micronucleus formation in polychromatic erythrocytes, simultaneously impairing hematological parameters along with erythrocyte antioxidants. The animals pre-treated with P. guajava exhibited a significant decrease in the COX-2 (P ≤ 0.01), IL-6 levels (P ≤ 0.05) and also displayed significant increase in the hepatic IL-10 levels (P ≤ 0.01). Pre-treatment with plant extract improved antioxidant enzyme activities, hematological parameters and reduced the intestinal damage by recovering the architecture of the small intestine. Moreover, extract also rendered protection against radiation induced DNA damage, as evidenced by the significant (P ≤ 0.01) decrease in the percentage of radiation-induced micronucleus in polychromatic erythrocytes. Furthermore, the herb extract treatment increased radiation LD50/30 from 6.6 Gy to 9.0 Gy, offering a dose reduction factor (DRF) of 1.36. Our findings for the first time propose the beneficial use of P. guajava as a radioprotector against X-ray induced damage.

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“…It presents a major challenge to discover a natural neuroprotective compound against radiation toxicity. Natural polyphenols are reported to relieve oxidative stress and neuroinflammation in glial cells . Quercetin is one such flavonoid that has been reported to have a wide array of biological functions, including anti‐inflammatory, anticarcinogenic, and antiviral activity .…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective effect of quercetin against radiation‐induced endoplasmic reticulum stress in neurons

Chatterjee

Langhnoja

Pillai

et al. 2018

J Biochem & Molecular Tox

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The endoplasmic reticulum (ER) plays an important role in the regulation and maintenance of cellular homeostasis. However, unresolved ER stress leads to deleterious effects by inducing the accumulation of unfolded proteins in the cell. Here we have demonstrated the protective aspects of quercetin against radiation-induced ER stress and against inflammation in primary cultured dorsal root ganglion (DRG) neurons. The mature DRG neurons were pretreated with different concentrations of quercetin (5-100 μM) for 24 hours before 2 Gy gamma radiation exposure and then subjected to a cytotoxicity assay, quantitative real-time polymerase chain reaction and Western blot analysis. The results showed that quercetin decreased the expression of BiP and C/EBP-homologous protein, the ER stress marker genes along with downregulation of tumor necrosis factor-α, JNK in irradiated DRG neurons. Furthermore, quercetin pretreatment significantly increased the cytoskeletal protein Tuj1 and the neurotrophin brain-derived neurotrophic factor in the neuron. These results indicate that quercetin plays a neuroprotective role against radiation-mediated ER stress and inflammatory responses. K E Y W O R D S dorsal root ganglion (DRG) neuron, endoplasmic reticulum (ER) stress, gamma radiation, inflammation, quercetin J Biochem Mol Toxicol. 2019;33:e22242.wileyonlinelibrary.com/journal/jbt

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Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach

Cited by 26 publications

References 34 publications

Anticlastogenic, radiation antagonistic, and anti-inflammatory activities of Persea americana in albino Wistar rat model

Anticlastogenic, radiation antagonistic, and anti-inflammatory activities of Persea americana in albino Wistar rat model

Radiation mitigating activities of Psidium guajava L. against whole-body X-ray-induced damages in albino Wistar rat model

Neuroprotective effect of quercetin against radiation‐induced endoplasmic reticulum stress in neurons

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