Radioprotection of deinococcal exopolysaccharide BRD125 by regenerating hematopoietic stem cells

Abstract

There is a substantial need for the development of biomaterials for protecting hematopoietic stem cells and enhancing hematopoiesis after radiation damage. Bacterial exopolysaccharide (EPS) has been shown to be very attractive to researchers as a radioprotectant owing to its high antioxidant, anti-cancer, and limited adverse effects. In the present study, we isolated EPS from a novel strain, Deinococcus radiodurans BRD125, which produces EPS in high abundance, and investigated its applicability as a radioprotective biomaterial. We found that EPS isolated from EPS-rich D. radiodurans BRD125 (DeinoPol-BRD125) had an excellent free-radical scavenging effect and reduced irradiation-induced apoptosis. In addition, bone-marrow and spleen-cell apoptosis in irradiated mice were significantly reduced by DeinoPol-BRD125 administration. DeinoPol-BRD125 enhanced the expression of hematopoiesis-related cytokines such as GM-CSF, G-GSF, M-CSF, and SCF, thereby enhancing hematopoietic stem cells protection and regeneration. Taken together, our findings are the first to report the immunological mechanism of a novel radioprotectant, DeinoPol-BRD125, which might constitute an ideal radioprotective and radiation mitigating agent as a supplement drug during radiotherapy.

Comparative genomics of Deinococcus radiodurans: unveiling genetic discrepancies between ATCC 13939K and BAA-816 strains

“…Biomaterials from D. radiodurans , including carotenoids (deinoxanthin), exopolysaccharides (DeinoPol), and membrane vesicles, exhibit significant industrial promise. Due to their antioxidant and radioprotective properties, these derivatives can suit food, cosmetics, and pharmaceutical industries ( Farci et al, 2017 ; Jeong et al, 2020 ; Park et al, 2022 ; Han et al, 2023 ). The genetic materials of D. radiodurans have gained interest among researchers.…”

Table_1.XLSX

“…Biomaterials from D. radiodurans, including carotenoids (deinoxanthin), exopolysaccharides (DeinoPol), and membrane vesicles, exhibit significant industrial promise. Due to their antioxidant and radioprotective properties, these derivatives can suit food, cosmetics, and pharmaceutical industries (Farci et al, 2017;Jeong et al, 2020;Park et al, 2022;Han et al, 2023). The genetic materials of D. radiodurans have gained interest among researchers.…”

Table_3.XLSX

“…Biomaterials from D. radiodurans, including carotenoids (deinoxanthin), exopolysaccharides (DeinoPol), and membrane vesicles, exhibit significant industrial promise. Due to their antioxidant and radioprotective properties, these derivatives can suit food, cosmetics, and pharmaceutical industries (Farci et al, 2017;Jeong et al, 2020;Park et al, 2022;Han et al, 2023). The genetic materials of D. radiodurans have gained interest among researchers.…”