Radioprotective effect of green tea and grape seed extracts mixture on gamma irradiation induced immune suppression in male albino rats

El-Desouky, Wael; Hanafi, Amal; Abbas, Manal M.

doi:10.1080/09553002.2016.1254834

Cited by 16 publications

(11 citation statements)

References 41 publications

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“…Some assays were also conducted in the BJ human skin fibroblast cell line in order to confirm our observation in keratinocytes. The inflammatory process is a key consequence after gamma radiation exposure, which is characterized by the increased levels of pro-inflammatory cytokines, including IL-6, TNF-α, and IL-1β [75,76]. Our study detected the enhancement in these inflammatory cytokines in cells and mice after gamma radiation exposure.…”

Section: Discussionsupporting

confidence: 51%

Celastrol Alleviates Gamma Irradiation-Induced Damage by Modulating Diverse Inflammatory Mediators

Wang

Ahn

Alharbi

et al. 2020

IJMS

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The present study aimed to explore the possible radioprotective effects of celastrol and relevant molecular mechanisms in an in vitro cell and in vivo mouse models exposed to gamma radiation. Human keratinocytes (HaCaT) and foreskin fibroblast (BJ) cells were exposed to gamma radiation of 20 Gy, followed by treatment with celastrol for 24 h. Cell viability, reactive oxygen species (ROS), nitric oxide (NO) and glutathione (GSH) production, lipid peroxidation, DNA damage, inflammatory cytokine levels, and NF-κB pathway activation were examined. The survival rate, levels of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in blood, and p65 and phospho-p65 expression were also evaluated in mice after exposure to gamma radiation and celastrol treatment. The gamma irradiation of HaCaT cells induced decreased cell viability, but treatment with celastrol significantly blocked this cytotoxicity. Gamma irradiation also increased free radical production (e.g., ROS and NO), decreased the level of GSH, and enhanced oxidative DNA damage and lipid peroxidation in cells, which were effectively reversed by celastrol treatment. Moreover, inflammatory responses induced by gamma irradiation, as demonstrated by increased levels of IL-6, TNF-α, and IL-1β, were also blocked by celastrol. The increased activity of NF-κB DNA binding following gamma radiation was significantly attenuated after celastrol treatment. In the irradiated mice, treatment with celastrol significantly improved overall survival rate, reduced the excessive inflammatory responses, and decreased NF-κB activity. As a NF-κB pathway blocker and antioxidant, celastrol may represent a promising pharmacological agent with protective effects against gamma irradiation-induced injury.

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Section: Discussionsupporting

confidence: 51%

Celastrol Alleviates Gamma Irradiation-Induced Damage by Modulating Diverse Inflammatory Mediators

Wang

Ahn

Alharbi

et al. 2020

IJMS

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“…Furthermore, Ahmed et al demonstrated that green tea by-products (contain 9.22% moisture, 20.1% crude protein, 2.91% crude fat, 18.2% crude fiber, 4.88% crude ash, 33.2% nitrogen-free extract, and 11.6% catechins) supplemented at the ratio of 0.5%, 1.0%, or 2.0% in diet positively modulated the proliferation of immune cells in goats in a linear mode [102]. On the other hand, tea can interact with other natural products to regulate the immune response [50,103]. In one study, the mixture of green tea and grape seed extract (100: 200 mg/kg BW) relieved the immune suppression induced by γ-irradiation in male rats, showing radioprotective effect [103].…”

Section: Immuno-regulative Activity In Vivomentioning

confidence: 99%

“…On the other hand, tea can interact with other natural products to regulate the immune response [50,103]. In one study, the mixture of green tea and grape seed extract (100: 200 mg/kg BW) relieved the immune suppression induced by γ-irradiation in male rats, showing radioprotective effect [103]. In another study, the combined administration of green tea Se-TPS and Huo-ji polysaccharides (1:1, 300 mg/kg BW) exerted synergistic effects on improving the immune function in mice [50].…”

Section: Immuno-regulative Activity In Vivomentioning

confidence: 99%

Health Functions and Related Molecular Mechanisms of Tea Components: An Update Review

Tang

Meng

Gan

et al. 2019

IJMS

271

150

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Tea is widely consumed all over the world. Generally, tea is divided into six categories: White, green, yellow, oolong, black, and dark teas, based on the fermentation degree. Tea contains abundant phytochemicals, such as polyphenols, pigments, polysaccharides, alkaloids, free amino acids, and saponins. However, the bioavailability of tea phytochemicals is relatively low. Thus, some novel technologies like nanotechnology have been developed to improve the bioavailability of tea bioactive components and consequently enhance the bioactivity. So far, many studies have demonstrated that tea shows various health functions, such as antioxidant, anti-inflammatory, immuno-regulatory, anticancer, cardiovascular-protective, anti-diabetic, anti-obesity, and hepato-protective effects. Moreover, it is also considered that drinking tea is safe to humans, since reports about the severe adverse effects of tea consumption are rare. In order to provide a better understanding of tea and its health potential, this review summarizes and discusses recent literature on the bioactive components, bioavailability, health functions, and safety issues of tea, with special attention paid to the related molecular mechanisms of tea health functions.

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“…Grape seed extract (GSE) was prepared according to Ahn et al and El-Desouky et al [44,45]. Grape seeds (GS) were dried at 50°C for 3 days in drying oven.…”

Section: Green Tea and Grape Seed Extracts Preparationmentioning

confidence: 99%

“…The obtained extracts were mixed to prepare GTE-GSE mixture with a ratio of 1:2 (100 mg GTE: 200 mg GSE). The mixture was determined previously by LC/DAD/MS HPLC and kept frozen at -20°C until further use [43,45].…”

Section: Green Tea and Grape Seed Extracts Preparationmentioning

confidence: 99%

Antiproliferative Effect of Green Tea and Grape Seed Mixture Extracts Combined with γ-irradiation on the Growth of Myeloma Cells

El-Shershaby

El-Desouky

Ebeid

2018

Arab Journal of Nuclear Sciences and Applications

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The purpose of the present work is to study the effect of green tea and grape seed extracts mixture (GTE-GSE mix) and γ-irradiation or their combination to enhance the antiproliferative effect on the growth of myeloma cells. To investigate this effect, a myeloma cell line SP2/OR was cultured in RPMI-1640 medium; total count and viability % were daily calculated. Myeloma cells (1 X 10 6 /ml) were treated with GTE-GSE mix concentrations (100 -1000 ng/ml), γ-irradiation doses (0.5 -10 Gy) and their combination for 6 days. Evaluation of Caspase 8 and 9 activities were measured. Cell culture media and GTE-GSE mix activity were determined using DPPH method.The results of this study indicate that the most effective dose of GTE-GSE mix on myeloma cells growth inhibition and viability % was 1000 ng/ml, while the effective dose of γ-irradiation was 8 Gy where viability percentage was zero. The obtained results indicate that treatment with GTE-GSE mix plus γ-irradiation combination (700 ng/ml + 6 Gy) resulted in zero% growth inhibition at 4 th day. Treatment with combination dose induced activation of caspase 8 and caspase 9 levels more than GTE-GSE mix and γ-irradiation alone. Treatment with GTE-GSE mix and γ-irradiation individually resulted in a significant reduction in DPPH activity to 62% while in combination treatment was 73.5%. It could be concluded that the treatment with GTE-GSE mix plus γ-irradiation (700 ng/ml + 6 Gy) combination resulted in a marked increase of growth inhibition levels, apoptosis and cancer cells regression when compared with each agent alone. GTE-GSE mix plus γirradiation combination had an antiproliferative effect on the growth of myeloma cells.

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Radioprotective effect of green tea and grape seed extracts mixture on gamma irradiation induced immune suppression in male albino rats

Abstract: GTE and GSE mixture is a good radioprotector and immune modulator compound, indicating its possible use as an adjuvant during radiotherapy.

Cited by 16 publications

References 41 publications

Celastrol Alleviates Gamma Irradiation-Induced Damage by Modulating Diverse Inflammatory Mediators

Celastrol Alleviates Gamma Irradiation-Induced Damage by Modulating Diverse Inflammatory Mediators

Health Functions and Related Molecular Mechanisms of Tea Components: An Update Review

Antiproliferative Effect of Green Tea and Grape Seed Mixture Extracts Combined with γ-irradiation on the Growth of Myeloma Cells

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