Radioprotective effect of radiation-induced Lactococcus lactis cell-free extract against 60Coγ injury in mice

Abstract

Ionizing radiation (IR) is widely used in the diagnosis and treatment of various cancers. However, IR can cause damage to human health by producing reactive oxygen species. Lactococcus lactis is a type of microorganism that is beneficial to human health and has a strong antioxidant capacity. In this study, the protective effect of normal and IR-induced L. lactis IL1403 cell-free extracts (CFE and IR-CFE, respectively) against oxidative damage in vitro and the radioprotective effect of IR-CFE in vivo was evaluated using 60 Coγ-induced oxidative damage model in mice. Results showed that IR-CFE exhibited a stronger oxidative damage-protective effect than CFE for L. lactis IL1403 under H 2 O 2 in vitro. Moreover, IR-CFE also showed strong radioprotective effect on hepatocyte cells (AML-12) under radiation condition, and the effect was better than that of CFE. Animal experiment indicated that IR-CFE could reduce the IR-induced damage to the hematopoietic system by increasing the number of white blood cells and red blood cells in peripheral blood of irradiated mice. It was also observed that IR-CFE could markedly alleviate the 60 Coγ-induced oxidative stress via increasing the activities of superoxide dismutase and glutathione peroxidase, enhancing the levels of glutathione, and decreasing the contents of malondialdehyde in serum, liver, and spleen. In addition, IR-CFE also could reduce the activities of alanine transaminase and aspartate aminotransferase in serum, thereby reducing radiation damage to the liver. These results suggested that IR-CFE could be considered as potential candidates for natural radioprotective agents. This study provides a theoretical basis for improving the application of lactic acid bacteria.

Extraction, characterization, and biological activities of a polysaccharide from Poria cocos peels

“…Ionizing radiation could produce excessive free radicals, which damage immune organs and cells, thus causing the abnormal immune function of the body. Previous studies had reported that the mechanism of radiation‐induced liver injury was related to immunomodulatory effects (Kang et al, 2019; Suo et al, 2021). It was shown in Figure 6a–c that PCPP‐1A exhibited good immunomodulatory activity in vitro .…”

Radioprotective effect of Malva sylvestris L. against radiation-induced liver, kidney and intestine damages in rat: A histopathological study

“…The use of radioprotectors could improve the therapeutic gain of radiation therapy [ 8 ]. Various radioprotectors have been introduced that can be very effective and helpful in mitigating the side effects of IR [ [9] , [10] , [11] , [12] , [13] , [14] ]. Flavonoids are a group of natural polyphenolic compounds that have been identified in many plants in different species and can have radiation protection properties [ 15 , 16 ].…”